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note
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For the probe aperture diameters we relied on the data provided by the manufacturer, who is using scanning electron microscopy for the determination of the aperture size of each probe. Although we cannot completely rule out a widening of the aperture after the first shear-force interaction between the probe and the substrate, or as a result of UV illumination, we have not witnessed a change in the transmission rate, during the scans, that would be suggestive of an aperture broadening. This is in line with the observation that the Jasco probes are the most durable we have used.
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note
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Note that AFM imaging before and after the thermal conversion treatment show a reduction in size of the precursor features upon conversion, as expected on the basis of the tetrahydrothiophenium elimination. We can thus rule out the thermal conversion as the reason for the discrepancy between the probe size and the feature size.
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