Caged RNase: Photoactivation of the enzyme from perfect off-state by site-specific incorporation of 2-nitrobenzyl moiety

Bioorganic and Medicinal Chemistry Letters

Volumn 13, Issue 1, 2003, Pages 13-15

  Hiraoka, Takashi ^a   Hamachi, Itaru ^a  

^a KYUSHU UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ASPARTIC ACID; BENZYL DERIVATIVE; GLUTAMIC ACID; GLUTAMINE; LYSINE; MUTANT PROTEIN; O NITROBENZYL; RIBONUCLEASE; UNCLASSIFIED DRUG; VITRONECTIN; NITROBENZENE; NITROBENZENE DERIVATIVE; PEPTIDE FRAGMENT; RIBONUCLEASE S; RIBONUCLEASE S-PEPTIDE; S PEPTIDE;

ALPHA HELIX; AMINO ACID SEQUENCE; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME ACTIVITY; PHOTOACTIVATION; BINDING SITE; CHEMISTRY; METABOLISM; MUTATION; PHOTOLYSIS; RADIATION EXPOSURE; SITE DIRECTED MUTAGENESIS;

AMINO ACID SEQUENCE; BINDING SITES; MUTAGENESIS, SITE-DIRECTED; MUTATION; NITROBENZENES; PEPTIDE FRAGMENTS; PHOTOLYSIS; RIBONUCLEASES;

EID: 0037421039     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0960-894X(02)00825-9     Document Type: Article

References (20)

1. Walker J.W., Athey P.S., Kueper L.W., Matuzewski B., Xue J.Y. Methods Enzymol. 172:1989;288.
2. Hess G.P. Biochemistry. 32:1993;989.
3. Nerbonne J.M. Curr. Opin. Neurobiol. 6:1996;379.
4. Ayley H., Chang Y., Miller W.T., Niblack B., Pan P. Methods Enzymol. 291:1998;117.
5. (a) Jones P.B., Porter N.A. J. Am. Chem. Soc. 121:1999;2753 (b) Porter N.A., Thuring J.W., Liu H. J. Am. Chem. Soc. 121:1999;7716.
6. (a) Jones P.B., Porter N.A. J. Am. Chem. Soc. 121:1999;2753 (b) Porter N.A., Thuring J.W., Liu H. J. Am. Chem. Soc. 121:1999;7716.
7. Mendel D., Ellman J.A., Schultz P.G. J. Am. Chem. Soc. 113:1991;2758.
8. Self C.H., Thompson S. Nat. Med. 2:1996;817.
9. Zou K., Cheley S., Gives R.S., Bayley H. J. Am. Chem. Soc. 124:2002;8220.
10. Hamachi I., Hiraoka T., Yamada Y., Shinkai S. Chem. Lett. 1998;537.
11. Hamachi I., Yamada Y., Matsugi T., Shinkai S. Chem. Eur. J. 5:1999;1503.
12. Hamachi I., Yamada Y., Eboshi R., Hiraoka T., Shinkai S. Bioorg. Med. Chem. Lett. 9:1999;1215.
13. Hamachi I., Eboshi R., Watanabe J., Shinkai S. J. Am. Chem. Soc. 122:2000;4530.
14. Hamachi I., Eboshi R., Watanabe J., Hiraoka T., Shinkai S. Biopolymers. 55:2000;459.
15. 3OH: C, 62.68; H, 5.26; N, 5.22 Wieboldt R.G., Kyle R., Niu L., Ramesh D., Carpenter B.K., Hess G.P. Proc. Natl. Acad. Sci. U.S.A. 91:1994;8752.
16. 2O: C, 59.68; H, 5.70; N, 7.20 Tatsu Y., Shigeri Y., Ishida A., Kameshita I., Fujisawa H., Yumoto N. Bioorg. Med. Chem. Lett. 9:1999;1093.
17. 2O: C, 60.50; H, 5.58; N, 7.06 Ramesh D., Wieboldt R., Billington A.P., Carpenter B.K., Hess G.P. J. Org. Chem. 58:1993;4599.
18. +=1864.9, obsd 1865.1.
19. delCardayre S.B., Raines R.T. Biochemistry. 33:1994;989.
20. Raines R.T. Chem. Rev. 98:1998;1045. and references therein.