Single-or dual-mode switching of semisynthetic ribonuclease S′ with an iminodiacetic acid moiety in response to the copper(II) concentration

Chemistry - A European Journal

Volumn 5, Issue 5, 1999, Pages 1503-1511

  Hamachi, Itaru ^a   Yamada, Yasuhiro ^a   Matsugi, Tomoaki ^a   Shinkai, Seiji ^a  

^a KYUSHU UNIVERSITY   (Japan)

Author keywords

Amino acids; Copper; Enzyme catalysis; Ribonuclease; Switchable enzymes

Indexed keywords

ACETIC ACID; AMINO ACID; COPPER; RIBONUCLEASE;

ALPHA HELIX; AMINO ACID SEQUENCE; ARTICLE; CATALYSIS; CHEMICAL MODIFICATION; CHEMICAL REACTION; CIRCULAR DICHROISM; COMPLEX FORMATION; CONCENTRATION (PARAMETERS); CONFORMATION; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME STRUCTURE; HYDROLYSIS; PEPTIDE SYNTHESIS; STOICHIOMETRY; STRUCTURE ANALYSIS; SYNTHESIS;

EID: 0032941048     PISSN: 09476539     EISSN: None     Source Type: Journal    
DOI: 10.1002/(sici)1521-3765(19990503)5:5<1503::aid-chem1503>3.0.co;2-6     Document Type: Article

References (64)

1. S. J. Lippard, J. M. Berg, in Principles of Bioinorganic Chemistry, University Science Books, Mill Valley, CA, 1994.
2. J. W. Bryson, S. F. Betz, H. S. Lu, D. J. Suich, H. X. Zhou, K. T. O'Neil, W. F. DeGrado, Science 1995, 270, 935.
3. a) M. R. Ghadiri, C. Choi, J. Am. Chem. Soc. 1990, 112, 1630;
4. b) M. R. Ghadiri, A. K. Fernholz, J. Am. Chem. Soc. 1990, 112, 9633;
5. c) M. Lieberman, T. Sasaki, J. Am. Chem. Soc. 1991, 113, 1470;
6. d) M. R. Ghadiri, C. Soares, C. Choi, J. Am. Chem. Soc. 1992, 114, 825;
7. e) H. Mihara, N. Nishino, T. Fujimoto, Chem. Lett. 1992, 1809;
8. f) H. Mihara, N. Nishino, R. Hasegawa, T. Fujimoto, S. Usui, H. Ishida, K. Ohkubo, Chem. Lett. 1992, 1813;
9. g) B. Cuenoud, A. Schepartz, Science 1993, 259, 510;
10. h) W. D. Kohn, C. M. Kay, B. D. Sykes, R. S. Hodges, J. Am. Chem. Soc. 1998, 120, 1124.
11. F. Ruan, Y. Chen, P. B. Hopkins, J. Am. Chem. Soc. 1990, 112, 9403.
12. a) R. Kuroki, Y. Taniyama, C. Seko, H. Nakamura, M. Kikuchi, M. Ikehara, Proc. Natl. Acad. Sci. USA 1989, 86, 6903;
13. b) J. N. Higaki, B. L. Haymore, S. Chen, R. J. Fletterick, C. S. Craik, Biochemistry 1990, 29, 8582;
14. c) J. N. Higaki, R. J. Fletterick, C. S. Craik, Trends Biochem. Sci. 1992, 17, 100;
15. d) D. J. Matthews, Curr. Opin. Biotechnol. 1995, 6, 419;
16. e) V. A. Roberts, B. L. Iverson, S. A. Iverson, S. J. Benkovic, R. A. Lerner, E. D. Getzoff, J. A. Tainer, Proc. Natl. Acad. Sci. USA 1990, 87, 6654;
17. f) B. L. Iverson, S. A. Iverson, V. A. Roberts, E. D. Getzoff, J. A. Tainer, S. J. Benkovic, R. A. Lerner, Science 1990, 249, 659;
18. g) D. S. Gregory, A. C. R. Martin, J. C. Cheetham, A. R. Ress, Protein Eng.. 1993, 6, 29;
19. h) W. S. Wada, J. S. Koh, N. Han, D. M. Hoekstra, R. A. Lerner, J. Am. Chem. Soc. 1993, 115, 4449;
20. i) C. F. Barbas III, J. S. Rosenblum, R. A. Lerner, Proc. Natl. Acad. Sci. USA 1993, 90, 6385;
21. j) J. D. Stewart, V. A. Robberts, M. W. Crowder, E. D. Getzof, S. J. Benkovic, J. Am. Chem. Soc. 1994, 116, 415;
22. k) A. L. Pinto, H. W. Hellinga, J. H. Caradonna, Proc. Natl. Acad. Sci. USA 1997, 94, 5562;
23. l) C. D. Coldren, H. W. Hellinga, J. H. Caradonna, Proc. Natl. Acad. Sci. USA 1997, 94, 6635.
24. R. T. Raines, Chem. Rev. 1998, 98, 1045 and references therein.
25. a) B. Imperiali, R. S. Roy, J. Am. Chem. Soc. 1994, 116, 12083;
26. b) R. S. Roy, B. Imperiali, Protein Eng.. 1997, 10, 691;
27. c) I. Hamachi, T. Hiraoka, Y. Yamada, S. Shinkai, Chem. Lett. 1998, 537.
28. F. Ruan, Y. Chen, K. Itoh, T. Sasaki, P. B. Hopkins, J. Org. Chem. 1991, 56, 4347.
29. H. W. Wyckoff, D. Tsernoglou, A. W. Hanson, J. R. Knox, B. Lee, F. M. Richards, J. Biol. Chem. 1970, 245, 305.
30. a) E. E. Kim, R. Varadarajan, H. W. Wyckoff, F. M. Richards, Biochemistry 1992, 31, 12304;
31. b) E. Scoffone, F. Rocchi, F. Marchiori, L. Moroder, A. Marzotto, A. M. Tamburro, J. Am. Chem. Soc. 1967, 89, 5450;
32. c) F. M. Finn, K. Hoffman, J. Am. Chem. Soc. 1965, 87, 645.
33. a) N. E. Zhou, C. M. Kay, R. S. Hodges, J. Biol. Chem. 1992, 267, 1992;
34. b) R. Walgers, T. C. Lee, A. C. Goodwin, J. Am. Chem. Soc. 1998, 120, 5073.
35. -1. A. E. Martell, R. M. Smith, in Crystal Stability Constants, Vol. 1, Plenum, New York, 1989, p. 118.
36. II under the same conditions.
37. II cation.
38. a) U. W. Kenkare, F. M. Richard, J. Biol. Chem. 1966, 241, 3197;
39. b) B. M. Dunn, C. Dibello, K. L. Kirk, L. A. Cohen, I. M. Chaiken, J. Biol. Chem. 1974, 249, 6295;
40. c) M. Irie, K. Ohgi, M. Yoshinaga, T. Yanagida, Y. Okada, N. Teno, J. Biochem. 1986, 100, 1057.
41. It has been reported that replacement of Met13 and Asp14 with hydrophilic residues markedly lowers the enzymatic activity. a) P. R. Connelly, R. Varadarajan, J. M. Sturtevant, F. M. Richards, Biochemistry 1990, 29, 6108;
42. b) K. Hoffman, F. M. Finn, M. Limetti, J. Montibeller, G. Zanetti, J. Am. Chem. Soc. 1966, 88, 3633.
43. All of the binding constants were calculated with a nonlinear curve fitting program.
44. F. M. Richards, P. J. Vithayathil, J. Biol. Chem. 1959, 234, 1459.
45. B. M. Dunn, C. Dibello, K. L. Kirk, L. A. Cohen, I. M. Chaiken, J. Biol. Chem. 1974, 249, 6295.
46. II complexes are not clear so far.
47. II).
48. Tyr residues are located at positions 25, 73, 76, 92, 97 and 115 of the S-protein portion.
49. II cation does not show any RNase activity monitored by the hydrolysis reaction of poly-U under the present conditions.
50. All measurements of the RNase activity assay were performed with 1 μM of RNase S′.
51. II.
52. -4M. a) T. Takahashi, M. Irie, T. Ukita, J. Biochem. 1967, 61, 669;
53. II.
54. a) I. Hamachi, Y. Tajiri, S. Shinkai, J. Am. Chem. Soc. 1994, 116, 7437;
55. b) I. Hamachi, Y. Tajiri, T. Nagase, S. Shinkai, Chem. Eur. J. 1997, 3, 1025;
56. c) I. Hamachi, T. Nagase, Y. Tajiri, S. Shinkai, Chem. Commun. 1996, 281;
57. d) I. Hamachi, T. Nagase, Y. Tajiri, S. Shinkai, Bioconjugate Chem. 1997, 8, 862;
58. e) I. Hamachi, T. Matsugi, K. Wakigawa, S. Shinkai, Inorg. Chem. 1998, 37, 1592.
59. As other switchable enzyme systems, photoswitchable proteins have been reported; a) I. Willner, S. Rubin, Angew. Chem. 1996, 108, 419; Angew. Chem. Int. Ed. Engl. 1996, 35, 367;
60. As other switchable enzyme systems, photoswitchable proteins have been reported; a) I. Willner, S. Rubin, Angew. Chem. 1996, 108, 419; Angew. Chem. Int. Ed. Engl. 1996, 35, 367;
61. b) I. Hamachi, S. Tanaka, S. Shinkai, J. Am. Chem. Soc. 1993, 115, 10458;
62. c) I. Hamachi, S. Tanaka, S. Tsukiji, S. Shinkai, M. Shimizu, T. Nagamune, Chem. Commun. 1997, 1735.
63. M. S. Doscher, C. H. W. Hirs, Biochemistry 1967, 6, 304.
64. S. B. delCardayre, R. T. Raines, Biochemistry 1994, 33, 6031.