Selective inhibition, separation, and purification of serine proteases: A strategy based on a photoremovable inhibitor [11]

Journal of the American Chemical Society

Volumn 121, Issue 33, 1999, Pages 7716-7717

  Porter, Ned A ^a   Thuring, Jan Willem ^b   Li, Hui ^b  

^a VANDERBILT UNIVERSITY   (United States)

^b NONE

Author keywords

[No Author keywords available]

Indexed keywords

BLOOD CLOTTING FACTOR 10A; BLOOD CLOTTING FACTOR 10A INHIBITOR; CINNAMIC ACID DERIVATIVE; SERINE PROTEINASE; THROMBIN; THROMBIN INHIBITOR;

BLOOD CLOTTING; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME PURIFICATION; ENZYME SUBSTRATE; LETTER; LIGHT; PHOTOACTIVATION;

EID: 0033603895     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja990802y     Document Type: Letter

References (22)

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2. Porter, N. A.; Bruhnke, J. D. Photochem. Photobiol. 1990, 51, 37-43.
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7. (a) Porter, N. A.; Bruhnke, J. D.; Koenigs, P. In Bioorganic Photochemistry; Morrison, H., Ed.; John Wiley & Sons: New York, 1993; Vol. II, p 197-240.
8. (b) For an excellent review of the use of light to control biological processes, see: Willner, I.; Rubin, S. Angew. Chem., Int. Ed. Engl. 1996, 38, 367-385.
9. Arroyo, J. G.; Jones, P. B.; Porter, N. A.; Hatchel, D. L. Thrombos. Haemostasis 1997, 78, 791-793.
10. The photochemistry involves a trans-cis photoisomerization followed by lactonization that occurs on the ms time scale, see refs 3a and 4.
11. Koenigs, P. M.; Faust, B. C.; Porter, N. A. J. Am. Chem. Soc. 1993, 115, 9371-9379.
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13. Details of the library synthesis and assay are described in Supporting Information.
14. 3 was Asp, Gly, Ile, Lys, Phe, Phg, Tyr, and Val.
15. 3 is identified by the single letter symbol.
16. The enzyme recoveries reported here are lower limits since a considerable amount of enzyme was consumed in assays of fractions.
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