Alphavirus cDNA-based expression vectors: Effects of RNA transcription and nuclear export

Biotechnology and Bioengineering

Volumn 81, Issue 5, 2003, Pages 553-562

  Boorsma, Marco ^a,b   Saudan, Philippe ^a   Pfruender, Holger ^a   Bailey, James E ^a   Schlesinger, Sondra ^c   Renner, Wolfgang A ^a   Bachmann, Marlin F ^a  

^a CYTOS BIOTECHNOLOGY AG   (Switzerland)

^b ETH ZURICH   (Switzerland)

^c WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Nuclear export; Regulated expression; RNA replication

Indexed keywords

GENES; PROTEINS; RNA; TEMPERATURE; VACCINES; VIRUSES;

ALPHAVIRUS VECTORS;

DNA;

COMPLEMENTARY DNA; DNA; RNA; VIRUS VECTOR;

ALPHA VIRUS; ANIMAL CELL; ARTICLE; CELL ASSAY; CELL LINE; CELL PROLIFERATION; CELL STRAIN BHK; CHO CELL; CONTROLLED STUDY; CYTOPLASM; DNA VECTOR; FIBROBLAST; GENE EXPRESSION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; NONHUMAN; PROTEIN SYNTHESIS; REGULATORY MECHANISM; REPLICON; RNA ANALYSIS; RNA REPLICATION; RNA TRANSCRIPTION; RNA TRANSPORT; TEMPERATURE; THERMOREGULATION; VIRUS INFECTION;

ACTIVE TRANSPORT, CELL NUCLEUS; ALPHAVIRUS; ANIMALS; CELLS, CULTURED; CHO CELLS; CLONING, MOLECULAR; CRICETINAE; DNA, COMPLEMENTARY; DNA, VIRAL; GENE EXPRESSION REGULATION; GENETIC VECTORS; KIDNEY; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS; REPLICON; RNA, VIRAL; SINDBIS VIRUS; TRANSCRIPTION, GENETIC; TRANSFECTION;

ALPHAVIRUS;

EID: 0037420369     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.10496     Document Type: Article

References (28)

1. Agapov EV, Frolov I, Lindenbach BD, Pragai BM, Schlesinger S, Rice CM. 1998. Non-cytopathic Sindbis virus RNA vectors for heterologous gene expression. Proc Natl Acad Sci USA 95:12989-12994.
2. Berger J, Hauber J, Hauber R, Geiger R, Cullen BR. 1988. Secreted placential alkaline phosphatases: a powerful new quantitative indicator of gene expression in eukaryotic cells. Gene 66:1-10.
3. Boorsma M, Nieba L, Koller D, Bachmann MF, Bailey JE, Renner WA. 2000. A temperature-regulated replicon-based DNA expression system. Nat Biotechnol 8:429-432.
4. Braun IC, Rohrbach E, Schmitt C, Izaurralde E. 1999. TAP binds to the constitutive transport element (CTE) through a novel RNA-binding motif that is sufficient to promote CTE-dependent RNA export from the nucleus. EMBO J 18:1953-1965.
5. Bredenbeek PJ, Frolov I, Rice CM, Schlesinger S. 1993. Sindbis virus expression vectors: packaging of RNA replicons by using defective helper RNAs. J Virol 67:6439-6446.
6. Buchman AR, Berg P. 1988. Comparison of intron-dependent and intron-independent gene expression. Mol Cell Biol 8:4395-4401.
7. DiCiommo DP, Bremner R. 1998. Rapid, high-level protein production using DNA-based Semliki forest virus vectors. J Biol Chem 273: 18060-18066.
8. Dorner AJ, Wasley LC, Kaufman RJ. 1989. Increased synthesis of secreted proteins induces expression of glucose-regulated proteins in butyrate-treated Chinese hamster ovary cells. J Biol Chem 264:20602-20607.
9. Dryga SA, Dryga OA, Schlesinger S. 1997. Identification of mutations in a Sindbis virus variant able to establish persistent infection in BHK cells: the importance of a mutation in the nsP2 gene. Virology 228: 74-83.
10. Dubensky TW, Driver DA, Polo JM, Belli BA, Latham EM, Ibanez CE, Chada S, Brumm D, Banks TA, Mento SJ, Jolly DJ, Chang SMW. 1996. Sindbis virus DNA-based expression vectors: utility for in vitro and in vivo gene transfer. J Virol 70:508-519.
11. Frolov I, Agapov E, Hoffman TA, Pragai BM, Lippa M, Schlesinger S, Rice CM. 1999. Selection of RNA replicons capable of persistent noncytopathic replication in mammalian cells. J Virol 73:3854-3865.
12. Fussenegger M. 2001. The impact of mammalian gene regulation concepts on functional genomics research, metabolic engineering, and advanced gene therapies. Biotechnol Prog 17:1-51.
13. Fussenegger M, Bailey JE, Hauser H, Mueller PP. 1999. Genetic optimization of recombinant glycoprotein production by mammalian cells. Trends Biotechnol 17:35-42.
14. Herweijer H, Latendress JS, Williams P, Zhang G, Danko I, Schlesinger S, Wolff JA. 1995. A plasmid-based self-amplifying Sindbis virus vector. Hum Gene Ther 6:1161-1167.
15. Huang MTF, Gorman CM. 1990. Intervening sequences increase efficiency of RNA 3′ processing and accumulation of cytoplasmic RNA. Nucleic Acids Res 18:937-947.
16. Kaufmann H, Mazur X, Marone R, Bailey JE, Fussenegger M. 1999. Comparative analysis of two controlled proliferation strategies regarding product quality, influence on tetracycline-regulated gene expression, and productivity. Biotechnol Bioeng 72:592-602.
17. Kruh J. 1982. Effects of sodium butyrate, a new pharmaceutical agent, on cells in culture. Mol Cell Biochem 42:65-82.
18. Lundström K. 2000. Semliki forest virus vectors: versatile applications in neurobiology. Res Adv Neurochem 1:1-11.
19. Makrides SC. 1999. Components of vectors for gene transfer and expression in mammalian cells. Protein Expression Purif 17:183-202.
20. Martinez-Salas E. 1999. Internal ribosome entry site biology and its use in expression vectors. Curr Opin Biotechnol 10:458-464.
21. Mazur X, Fussenegger M, Renner WA, Bailey JE. 1998. Higher productivity of growth-arrested Chinese hamster ovary cells expressing the cyclin-dependent kinase inhibitor p27, Biotechnol Prog 14:705-713.
22. Perri S, Driver DA, Gardner JP, Sherrill S, Belli BA, Dubensky TW, Polo JM. 2000. Replicon vectors derived from Sindbis virus ar d Semliki forest virus that establish persistent replication in host cells. J Virol 74:9802-9807.
23. Polo JM, Belli BA, Driver DA, Frolov I, Sherrill S, Hariharan MJ, Townsend K, Perri S, Mento SJ, Jolly DJ, Chang SMW, Schlesinger S, Dubensky TW. 1999. Stable alphavirus packaging cell line; for Sindbis virus- and Semliki forest virus-derived vectors. Proc Natl Acad Sci USA 96:4598-4603.
24. Schlesinger S, Dubensky TW. 1999. Alphavirus vectors for gene expression and vaccines. Curr Opin Biotechnol 10:434-439.
25. Strauss JH, Strauss EG. 1994. The alphaviruses: gene expression, replication, and evolution. Microbiol Rev 58:491-562.
26. Strong TV, Hampton TA, Louro I, Bilbao G, Conry RM, Curiel DT. 1997. Incorporation of β-globin untranslated regions into a Sindbis virus vector for augmentation of heterologous mRNA expression. Gene Ther 4:624-627.
27. Xiong C, Levis P, Shen S, Schlesinger S. Rice CM, Huang HV. 1989. Sindbis virus: an efficient broad host range vector for gene expression in animal cells. Science 243:1188-1191.
28. Zufferey R, Donello JE, Trono D, Hope TJ. 1999. Woodchuck hepatitis virus posttranscriptional regulatory element enhances expression of transgenes delivered by retroviral vectors. J Virol 73:288-2892.