A temperature-regulated replicon-based DNA expression system

Nature Biotechnology

Volumn 18, Issue 4, 2000, Pages 429-432

  Boorsma, Marco ^a,b   Nieba, Lars ^a   Koller, Daniel ^a,b   Bachmann, Martin F ^a   Bailey, James E ^b   Renner, Wolfgang A ^a  

^a CYTOS BIOTECHNOLOGY AG   (Switzerland)

^b ETH ZURICH   (Switzerland)

Author keywords

Alphavirus; DNA vector; Sindbis virus; Stable expression; Temperature inducible gene expression; Toxic protein

Indexed keywords

RNA DIRECTED RNA POLYMERASE;

ALPHA VIRUS; ANIMAL CELL; ARTICLE; CELL STRAIN BHK; CHO CELL; GENE EXPRESSION; NONHUMAN; PRIORITY JOURNAL; REPLICON; TEMPERATURE DEPENDENCE;

ALPHAVIRUS; ANIMALS; CELL LINE; CHO CELLS; CRICETINAE; FEEDBACK; GENE EXPRESSION REGULATION, VIRAL; KINETICS; RECOMBINANT FUSION PROTEINS; REPLICON; RNA REPLICASE; TEMPERATURE; TRANSFECTION;

ALPHAVIRUS; ANIMALIA; SINDBIS VIRUS;

EID: 0034006644     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/74493     Document Type: Article

References (26)

1. Cregg, J.M., Vedvick, T.S. & Raschke, W.C. Recent advances in the expression of foreign genes in Pichia pastoris. Biotechnology 11, 905-910 (1993).
2. Makrides, S.C. Strategies for achieving high-level expression of genes in Escherichia coli. Microbiol. Rev. 60, 512-538 (1996).
3. Umāna, P., Jean-Mariet, J., Amstutz, H., Moudry, R. & Bailey, J.E. Engineered glycoforms of an anti-neuroblastoma lgG1 with optimized antibody-dependent cellular cytotoxic activity. Nat. Biotechnol. 17, 176-180 (1999).
4. Fussenegger, M., Schlatter, S., Datwijler, D., Mazur, X. & Bailey, J.E. Controlled proliferation by multigene metabolic engineering enhances the productivity of Chinese hamster ovary cells. Nat. Biotechnol. 16, 468-472 (1998).
5. Saez, E., No, D., West, A. & Evans, R.M. Inducible gene expression in mammalian cells and transgenic mice. Curr. Opin. Biotechnol. 8, 608-616 (1997).
6. Gossen, M. & Bujard, H. Tight control of gene expression in mammalian cells by tetracycline-response promoters. Proc. Natl. Acad. Sci USA 89, 5547-5551 (1992).
7. Mangelsdorf, D.J. et al. The nuclear receptor superfamily: the second decade. Cell 83, 835-839 (1995).
8. No, D., Yao, T & Evans, R.M. Ecdysone-inducible gene expression in mammalian cells and transgenic mice. Proc. Natl. Acad. Sci USA 93, 3346-3351 (1996).
9. Rossi, F.M.V. & Blau, H.M. Recent advances in inducible gene expression systems. Curr. Opin. Biotechnol. 9, 451-456 (1998).
10. Xiong, C. et al. Sindbis virus: an efficient, broad host range vector for gene expression in animal cells. Science 243, 1188-1191 (1989).
11. Schlesinger, S. Alphavirus vectors for the expression of heterologous genes. Trends Biotechnol. 11, 18-22 (1993).
12. Liljestrom, P. & Garoff, H. A new generation of animal cell expression vectors based on the Semliki Forest virus replicon. Bio/Technology 9, 1356-1361 (1991).
13. Liljestrbm, P. Alphavirus expression systems. Curr. Opin. Biotechnol. 5, 495-500 (1994).
14. Strauss, J.H. & Strauss, E.G. The alphaviruses: gene expression, replication and evolution. Microbiol. Rev. 58, 491-562 (1994).
15. Herwerjer et al. A plasmid-based self-amplifying Sindbis virus vector. Hum. Gene Ther. 6, 1495-1501 (1995).
16. Dubensky, T.W. et al. Sindbis virus DNA-based expression vectors: utility for in vitro and in vivo gene transfer. J. Virol. 70, 508-519 (1996).
17. Berglund, P., Tubelekas, I. & Liljeström, P. Alphaviruses as vectors for gene delivery. Trends Biotechnol. 14, 130-134 (1996).
18. Dryga, S.A., Dryga, O.A. & Schlesinger, S. Identification of mutations in a Sindbis virus variant able to establish persistent infection in BHK cells: the importance of a mutation in the nsP2 gene. Virology 228, 74-83 (1997).
19. Agapov, E.V. et al. Non-cytopathic Sindbis virus RNA vectors for heterologous gene expression. Proc. Natl. Acad. Sci. USA 95, 12989-12994 (1998).
20. Hahn, Y.S., Grakoui, A., Rice, C.M., Strauss, E.G. & Strauss, J.H. Mapping of RNA temperature-sensitive mutants of Sindbis virus: complementation group F mutants have lesions in nsP4. J. Virol. 63, 1194-1202 (1989).
21. Frolov, I. & Schlesinger, S. Translation of Sindbis virus mRNA: effects of sequences downstream of the initiating codon. J. Virol. 68, 8111-8117 (1994).
22. Sjöberg, E.M., Suomalainen, M. & Garoff, H. A significant improved Semliki Forest virus expression system based on translation enhancer segments from the viral capsid gene. Bio/Technology 12, 1127-1131 (1994).
23. Stanger, B.Z., Leder, P., Lee, T.H., Kim, E., & Seed, B. RIP: a novel protein containing a death domain that interacts with Fas/APO-1 (CD95) in yeast and causes cell death. Cell 81, 513-523 (1995).
24. Bredenbeek, P.J., Frolov, I., Rice, C.M. & Schlesinger, S. Sindbis virus expression vectors: packaging of RNA replicons by using defective helper RNAs. J. Virol. 67, 6439-6446 (1993).
25. Jordan, M., Schallhorn, A. & Wurm, F.M. Transfecting mammalian cells: optimization of critical parameters affecting calcium-phosphate precipitate formation. Nucleic Acid Res. 24, 596-601 (1996).
26. Berger, J., Hauber. J., Hauber, R., Geiger, R. & Cullen, B.R. Secreted placental alkaline phosphatases: a powerful new quantitative indicator of gene expression in eukaryotic cells. Gene 66, 1-10 (1988).