In vitro properties of RpoS (σs) mutants of Escherichia coli with postulated N-terminal subregion 1.1 or C-terminal region 4 deleted

Journal of Bacteriology

Volumn 185, Issue 8, 2003, Pages 2673-2679

  Gowrishankar, J ^a   Yamamoto, Kaneyoshi ^b,c   Subbarayan, P R ^b,d   Ishihama, Akira ^b,e  

^a CENTRE FOR DNA FINGERPRINTING AND DIAGNOSTICS   (India)

^b NATIONAL INSTITUTE OF GENETICS   (Japan)

^c KINKI UNIVERSITY   (Japan)

^d UNIVERSITY OF MIAMI MILLER SCHOOL OF MEDICINE   (United States)

^e NIPPON INSTITUTE FOR BIOLOGICAL SCIENCE   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

SIGMA FACTOR;

AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIUM MUTANT; CARBOXY TERMINAL SEQUENCE; DNA SUPERCOILING; ESCHERICHIA COLI; GEL MOBILITY SHIFT ASSAY; GENE DELETION; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN DOMAIN; TRANSCRIPTION INITIATION;

BACTERIAL PROTEINS; ESCHERICHIA COLI; GENE DELETION; PROMOTER REGIONS (GENETICS); PROTEIN SUBUNITS; SIGMA FACTOR; TEMPLATES, GENETIC; TRANSCRIPTION, GENETIC;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0037389328     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.185.8.2673-2679.2003     Document Type: Article

References (47)

1. Atlung, T., H. V. Nielsen, and F. G. Hansen. 2002. Characterisation of the allelic variation in the rpoS gene in thirteen K12 and six other non-pathogenic Escherichia coli strains. Mol. Genet. Genom. 266:873-881.
2. s. Mol. Microbiol. 39:1153-1165.
3. Bhandari, P., and J. Gowrishankar. 1997. An Escherichia coli host strain useful for efficient overproduction of cloned gene products with NaCl as the inducer. J. Bacteriol. 179:4403-4406.
4. 70 affects promoter DNA binding by Escherichia coli RNA polymerase holoenzyme. EMBO J. 18:709-716.
5. 70. J. Bacteriol. 182:221-224.
6. Burton, Z., R. R. Burgess, J. Lin, D. Moore, S. Holder, and C. A. Gross. 1981. The nucleotide sequence of the cloned rpoD gene for the RNA polymerase sigma subunit from E. coli K12. Nucleic Acids Res. 9:2889-2903.
7. Camarero, J. A., A. Shekhtman, E. A. Campbell, M. Chlenov, T. M. Gruber, D. A. Bryant, S. A. Darst, D. Cowburn, and T. W. Muir. 2002. Autoregulation of a bacterial σ factor explored by using segmental isotopic labeling and NMR. Proc. Natl. Acad. Sci. USA 99:8536-8541.
8. Campbell, E. A., O. Muzzin, M. Chlenov, J. L. Sun, C. A. Olson, O. Weinman, M. L. Trester-Zedlitz, and S. A. Darst. 2002. Structure of the bacterial RNA polymerase promoter specificity sigma subunit. Mol. Cell 9:527-539.
9. deHaseth, P. L., and J. D. Helmann. 1995. Open complex formation by Escherichia coli RNA polymerase: the mechanism of polymerase-induced strand separation of double helical DNA. Mol. Microbiol. 16:817-824.
10. Dombroski, A. J., W. A. Walter, and C. A. Gross. 1993. Amino-terminal amino acids modulate σ-factor DNA-binding activity. Genes Dev. 7:2446-2455.
11. 70 exhibit specificity of binding to promoter DNA. Cell 70:501-512.
12. Donahue, R. A., Jr., and R. L. Bebee. 1999. BL21-SI competent cells for protein expression in E coli. Focus 21:49-51.
13. 70 and disruption of its overall structure. Eur. J. Biochem. 244:613-618.
14. 70 abrogates productive transcription and restores its expected mobility on a denaturing gel. J. Mol. Biol. 242:9-22.
15. Gross, C. A., C. Chan, A. Dombroski, T. Gruber, M. Sharp, J. Tupy, and B. Young. 1998. The functional and regulatory roles of sigma factors in transcription. Cold Spring Harb. Symp. Quant. Biol. 63:141-155.
16. 70 to core RNA polymerase is a multistep process. Mol. Cell 8:21-31.
17. Hengge-Aronis, R. 2000. The general stress response in Escherichia coli, p. 161-178. In G. Storz and R. Hengge-Aronis (ed.), Bacterial stress responses. American Society for Microbiology, Washington, D.C.
18. Ishihama, A. 2000. Functional modulation of Escherichia coli RNA polymerase. Annu. Rev. Microbiol. 54:499-518.
19. Jishage, M., and A. Ishihama. 1997. Variation in RNA polymerase sigma subunit composition within different stocks of Escherichia coli W3110. J. Bacteriol. 179:959-963.
20. Juang, Y.-L., and J. D. Helmann. 1994. A promoter melting region in the primary σ factor of Bacillus subtilis. Identification of functionally important aromatic amino acids. J. Mol. Biol. 235:1470-1488.
21. 38 for overlapping promoters and ability to support CRP activation. Nucleic Acids Res. 23:819-826.
22. 70 subunit of Escherichia coli RNA polymerase in transcription activation. J. Mol. Biol. 235:405-413.
23. Kumar, A., R. A. Malloch, N. Fujita, D. A. Smillie, A. Ishihama, and R. S. Hayward. 1993. The minus 35-recognition region of Escherichia coli sigma 70 is inessential for initiation of transcription at an "extended minus 10" promoter. J. Mol. Biol. 232:406-418.
24. 70. J. Bacteriol. 177:5193-5196.
25. F holoenzyme involved in transcription of flagellar and chemotaxis genes. J. Bacteriol. 179:4264-4269.
26. 70 holoenzymes. Effect of DNA supercoiling. J. Biol. Chem. 271:1998-2004.
27. 70 family: sequence conservation and evolutionary relationships. J. Bacteriol. 174:3843-3849.
28. Malhotra, A., E. Severinova, and S. A. Darst. 1996. Crystal structure of a sigma 70 subunit fragment from E. coli RNA polymerase. Cell 87:127-136.
29. Melancon, P., R. R. Burgess, and M. T. Record, Jr. 1983. Direct evidence for the preferential binding of Escherichia coli RNA polymerase holoenzyme to the ends of deoxyribonucleic acid restriction fragments. Biochemistry 22: 5169-5176.
30. Murakami, K. S., S. Masuda, E. A. Campbell, O. Muzzin, and S. A. Darst. 2002. Structural basis of transcription initiation: an RNA polymerase holoenzyme-DNA complex. Science 296:1285-1290.
31. Murakami, K. S., S. Masuda, and S. A. Darst. 2002. Structural basis of transcription initiation: RNA polymerase holoenzyme at 4 Å resolution. Science 296:1280-1284.
32. 70 that are involved in interaction with RNA polymerase core enzyme. Genes Cells 2:725-734.
33. Ohnuma, M., N. Fujita, A. Ishihama, K. Tanaka, and H. Takahashi. 2000. A carboxy-terminal 16-amino-acid region of σ of Escherichia coli is important for transcription under high-salt conditions and sigma activities in vivo. J. Bacteriol. 182:4628-4631.
34. s) protein in Escherichia coli is active in vivo and exhibits normal environmental regulation even in the absence of rpoS transcriptional and translational control signals. J. Bacteriol. 184:3167-3175.
35. s-dependent promoter of the osmotically regulated proU operon of Salmonella typhimurium. J. Bacteriol. 179:7169-7173.
36. 70-directed transcription in vitro from osmotically regulated P1 and P2 promoters of proU in Escherichia coli. J. Bacteriol. 178:4176-4181.
37. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
38. Sharp, M. M., C. L. Chan, C. Z. Lu, M. T. Marr, S. Nechaev, E. W. Merritt, K. Severinov, J. W. Roberts, and C. A. Gross. 1999. The interface of σ with core RNA polymerase is extensive, conserved, and functionally specialized. Genes Dev. 13:3015-3026.
39. Shorenstein, R. G., and R. Losick. 1973. Comparative size and properties of the sigma subunits of ribonucleic acid polymerase from Bacillus subtilis and Escherichia coli. J. Biol. Chem. 248:6170-6173.
40. Studier, F. W., and B. A. Moffatt. 1986. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J. Mol. Biol. 189:113-130.
41. 38 (the rpoS gene product). Nucleic Acids Res. 23:827-834.
42. 38, is a second principal σ factor of RNA polymerase in stationaryphase Escherichia coli. Proc. Natl. Acad. Sci. USA 90:3511-3515.
43. Ueguchi, C., and T. Mizuno. 1993. The Escherichia coli nucleoid protein H-NS functions directly as a transcriptional repressor. EMBO J. 12:1039-1046.
44. Vassylyev, D. G., S.-I. Sekine, O. Laptenko, J. Lee, M. N. Vassylyeva, S. Borukhov, and S. Yokoyama. 2002. Crystal structure of a bacterial RNA polymerase holoenzyme at 2.6 Å resolution. Nature 417:712-719.
45. Visick, J. E., and S. Clarke. 1997. RpoS- and OxyR-independent induction of HPI catalase at stationary phase in Escherichia coli and identification of rpoS mutations in common laboratory strains. J. Bacteriol. 179:4158-4163.
46. 70 subunit of Escherichia coli RNA polymerase modulates the formation of stable polymerase/promoter complexes. J. Mol. Biol. 309:561-572.
47. 70 is required for efficient isomerization and initiation of transcription by Escherichia coli RNA polymerase. J. Mol. Biol. 267:60-74.