A fluorescence-based high-throughput assay for antimicrotubule drugs

Analytical Biochemistry

Volumn 315, Issue 1, 2003, Pages 49-56

  Barron, Donna M ^a,c   Chatterjee, Sabarni K ^a   Ravindra, Rudravajhala ^a   Roof, Rebecca ^a   Baloglu, Erkan ^b   Kingston, David G I ^b   Bane, Susan ^a  

^a BINGHAMTON UNIVERSITY   (United States)

^b VIRGINIA POLYTECHNIC INSTITUTE AND STATE UNIVERSITY   (United States)

^c REGENERON PHARMACEUTICALS INC   (United States)

Author keywords

Antimitotic; Colchicine; DAPI; High throughput assay; Microtubule; Polymerization; Taxol; Tubulin

Indexed keywords

BIOCHIPS; DRUG PRODUCTS; POLYMERIZATION; TURBIDITY;

ANTIMITOTIC; COLCHICINE; DAPI; HIGH-THROUGHPUT ASSAYS; MICROTUBULES; TAXOL; TUBULIN;

FLUORESCENCE;

ANTIMICROTUBULE DRUG; ANTINEOPLASTIC AGENT; TUBULIN; UNCLASSIFIED DRUG;

ABSORPTION; ACCURACY; ARTICLE; ASSAY; DRUG POTENCY; DRUG SCREENING; FLUORESCENCE SPECTROSCOPY; MICROTUBULE; MICROTUBULE ASSEMBLY; POLYMERIZATION; PRIORITY JOURNAL; RELIABILITY; REPRODUCIBILITY; TURBIDITY;

EID: 0037380829     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-2697(02)00691-7     Document Type: Article

References (24)

1. Wang L.G., Liu X.M., Kreis W., Budman D.R. The effect of antimicrotubule agents on signal transduction pathways of apoptosis: a review. Cancer Chemother. Pharmacol. 44:1999;355-361.
2. Shi Q., Chen K., Morris-Natschke S.L., Lee K.H. Recent progress in the development of tubulin inhibitors as antimitotic antitumor agents. Curr. Pharm. Des. 4:1998;219-248.
3. Jordan A., Hadfield J.A., Lawrence N.J., McGown A.T. Tubulin as a target for anticancer drugs: agents which interact with mitotic spindle. Med. Res. Rev. 18:1998;259-296.
4. Kruczynski A., Hill B.T. Vinflunine, the latest Vinca alkaloid in clinical development - A review of its preclinical anticancer properties. Crit. Rev. Oncol. Hematol. 40:2001;159-173.
5. Stachel S.J., Biswas K., Danishefsky S.J. The epothilones, eleutherobins, and related types of molecules. Curr. Pharm. Des. 7:2001;1277-1290.
6. Angerer E.V. New inhibitors of tubulin polymerisation. Exp. Opin. Ther. Patents. 9:1999;1069-1081.
7. Lin C.M., Singh S.B., Chu P.S., Dempcy R.O., Schmidt J.M., Pettit G.R., Hamel E. Interactions of tubulin with potent natural and synthetic analogs of the antimitotic agent combretastatin: a structure-activity study. Mol. Pharmacol. 34:1988;200-208.
8. Ingle J.D.J., Crouch S.R. Spectrochemical Analysis. 1988;Prentice Hall, Englewood Cliffs, NJ.
9. Kung C.E., Reed J.K. Fluorescent molecular rotors: a new class of probes for tubulin structure and assembly. Biochemistry. 28:1989;6678-6686.
10. ′, 6-Diamidino-2-phenylindole, a fluorescent probe for tubulin and microtubules J. Biol. Chem. 260:1985;2819-2825.
11. ′, 6-diamidino-2-phenylindole as a probe Eur. J. Biochem. 165:1987;613-620.
12. ′ NH/C-10 and C-2/C-10 modified paclitaxel analogs. Bioorg. Med. Chem. (2003) (in press).
13. Williams R.C. Jr., Lee J.C. Preparation of tubulin from brain. Methods Enzymol. 85:1982;376-385.
14. i by beef heart mitochondrial adenosine triphosphatase J. Biol. Chem. 252:1977;2891-2899.
15. Detrich III H.W., Williams R.C. Jr. Reversible dissociation of the. αβ dimer of tubulin from bovine brain Biochemistry. 17:1978;3900-3907.
16. Bhattacharyya B., Wolff J. Promotion of fluorescence upon binding of colchicine to tubulin. Proc. Natl. Acad. Sci. USA. 71:1974;2627-2631.
17. ′, 5,6,7-Substituted 2-phenyl-1,8-naphthyridin-4-ones: their synthesis, cytotoxicity, and inhibition of tubulin polymerization J. Med. Chem. 40:1997;2266-2275.
18. Staretz M.E., Hastie S.B. Synthesis, photochemical reactions and tubulin binding of novel photoaffinity labeling derivatives of colchicine. J. Org. Chem. 58:1993;1589-1592.
19. Vater W., Bohm K.J., Unger E. Effect of the fluorescence dye DAPI on microtubule structure in vitro: formation of novel types of tubulin assembly products. Acta Histochem. 94:1993;54-66.
20. Andreu J.M., Timasheff S.N. The measurement of cooperative protein self-assembly by turbidity and other techniques. Methods Enzymol. 130:1986;47-59.
21. Chatterjee S.K., Barron D.M., Vos S., Bane S. Baccatin III induces assembly of purified tubulin into long microtubules. Biochemistry. 40:2001;6964-6970.
22. Correia J.J., Lobert S. Physiochemical aspects of tubulin-interacting antimitotic drugs. Curr. Pharm. Des. 7:2001;1213-1228.
23. Timasheff S.N., J.M, Na G.C. Physical and spectroscopic methods for the evaluation of the interactions of antimitotic agents with tubulin. Pharmacol. Ther. 52:1991;191-210.
24. Robinson J., Engelborghs Y. Tubulin polymerization in dimethyl sulfoxide. J. Biol. Chem. 257:1982;5367-5371.