Estimate of the total number of CD8+ clonal expansions in healthy adults using a new DNA heteroduplex-tracking assay for CDR3 repertoire analysis

Journal of Immunological Methods

Volumn 274, Issue 1-2, 2003, Pages 159-175

  Bernardin, Flavien ^a,b   Doukhan, Laurence ^a,b   Longone Miller, Alcira ^a,b   Champagne, Patrick ^c   Sekaly, Rafick ^c   Delwart, Eric ^a,b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b Blood Centers of the Pacific   (United States)

^c UNIVERSITÉ DE MONTRÉAL   (Canada)

Author keywords

CDR3; DNA heteroduplex; Repertoire; T cell receptor

Indexed keywords

CD4 ANTIGEN; CD8 ANTIGEN; HETERODUPLEX; T LYMPHOCYTE RECEPTOR BETA CHAIN;

ACCURACY; ADULT; AMPLICON; ARTICLE; BLOOD CELL; CELL SELECTION; CLONAL VARIATION; COMPLEMENTARITY DETERMINING REGION; CONTROLLED STUDY; DNA SYNTHESIS; EFFECTOR CELL; FLOW CYTOMETRY; GENE SEQUENCE; HETERODUPLEX ANALYSIS; HUMAN; HUMAN CELL; IMMUNOASSAY; LYMPHOCYTE CLONE; MEMORY CELL; NORMAL HUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RNA EXTRACTION; RNA TRANSCRIPTION; T CELL RECEPTOR HETERODUPLEX TRACKING ASSAY;

ADULT; BASE SEQUENCE; CD8-POSITIVE T-LYMPHOCYTES; CLONE CELLS; COMPLEMENTARITY DETERMINING REGIONS; DNA PRIMERS; DNA PROBES; ELECTROPHORESIS; GENES, T-CELL RECEPTOR BETA; HETERODUPLEX ANALYSIS; HUMANS; LYMPHOCYTE ACTIVATION; LYMPHOCYTE COUNT; MIDDLE AGED; POLYMERASE CHAIN REACTION; RECEPTORS, ANTIGEN, T-CELL, ALPHA-BETA; REPRODUCIBILITY OF RESULTS; RNA, MESSENGER; SEQUENCE ANALYSIS, DNA; VARIATION (GENETICS);

EID: 0037373647     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(02)00514-8     Document Type: Article

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