Improved assessment of T-cell receptor (TCR) VB repertoire in clinical specimens: Combination of TCR-CDR3 spectratyping with flow cytometry-based TCR VB frequency analysis

Clinical and Diagnostic Laboratory Immunology

Volumn 9, Issue 2, 2002, Pages 257-266

  Pilch, H ^a   Hohn H ^a   Freitag, K ^a   Neukirch, C ^a   Necker, A ^a   Haddad, P ^a   Tanner, B ^a   Knapstein, P G ^a   Maeurer, M J ^a  

^a UNIVERSITY MEDICAL CENTER MAINZ   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIGEN; CD4 ANTIGEN; CD8 ANTIGEN; MAJOR HISTOCOMPATIBILITY ANTIGEN CLASS 1; T LYMPHOCYTE RECEPTOR; TETRAMER;

ANALYTIC METHOD; ARTICLE; CALIBRATION; CANCER IMMUNOLOGY; CASE REPORT; CELL SPECIFICITY; CELLULAR IMMUNITY; COMPLEMENTARITY DETERMINING REGION; CONTROLLED STUDY; DILUTION; FEMALE; FLOW CYTOMETRY; HUMAN; HUMAN TISSUE; IMMUNOASSAY; LYMPHOCYTE COUNT; PRIORITY JOURNAL; PROTEIN DOMAIN; QUALITATIVE ANALYSIS; QUANTITATIVE ANALYSIS; SPECTROMETRY; T LYMPHOCYTE; T LYMPHOCYTE SUBPOPULATION; UTERINE CERVIX CANCER; VACCINATION;

CD4-POSITIVE T-LYMPHOCYTES; CD8-POSITIVE T-LYMPHOCYTES; COMPLEMENTARITY DETERMINING REGIONS; FLOW CYTOMETRY; HUMANS; NEOPLASMS; RECEPTORS, ANTIGEN, T-CELL, ALPHA-BETA;

EID: 0036127889     PISSN: 1071412X     EISSN: None     Source Type: Journal    
DOI: 10.1128/CDLI.9.2.257-266.2002     Document Type: Article

References (37)

1. Phenotypic analysis of antigen-specific T lymphocytes
2. Analogues of CTL epitopes with improved MHC class-I binding capacity elicit anti-melanoma CTL recognizing the wild-type epitope
3. New methods for assessing T-cell responses
4. The composition of a primary T cell response is largely determined by the timing of recruitment of individual T cell clones
5. Antigen-selected T-cell receptor diversity and self-nonself homology
6. T-cell receptor repertoire in matched MART-1 peptide-stimulated peripheral blood lymphocytes and tumor-infiltrating lymphocytes
7. T-cell antigen receptor genes and T-cell recognition
8. An experimentally validated panel of subfamily-specific oligonucleotide primers (V alpha 1-w29/V beta 1-w24) for the study of human T cell receptor variable V gene segment usage by polymerase chain reaction
9. Perturbation of CD4+ and CD8+ T-cell repertoires during progression to AIDS and regulation of the CD4+ repertoire during antiviral therapy
10. Human papillomavirus type 33 E7 peptides presented by HLA-DR*0402 to tumor-infiltrating T cells in cervical cancer
11. Monoclonal TCR mRNA transcripts are preferentially detected in the TCR variable alpha chain in CD8(+) T-lymphocytes: Implications for immunomonitoring
12. Clonal expansion of Melan A-specific cytotoxic T lymphocytes in a melanoma patient responding to continued immunization with melanoma-associated peptides
13. Regression of human metastatic renal cell carcinoma after vaccination with tumor cell-dendritic cell hybrids
14. Examination of CD8+ T cell function in humans using MHC class I tetramers: Similar cytotoxicity but variable proliferation and cytokine production among different clonal CD8+ T cells specific to a single viral epitope
15. Peptides derived from self-protcins as partial agonists and antagonists of human CD8+ T-cell clones reactive to melanoma/melanocytc epitope MART1(27-35)
16. Direct evidence to support the immunosurveillance concept in a human regressive melanoma
17. T cell receptor usage of virus-specific CD8 cells and recognition of viral mutations during acute and persistent hepatitis B virus infection
18. Single-cell PCR analysis of TCR repertoires selected by antigen in vivo: A high magnitude CD8 response is comprised of very few clones
19. Cancer vaccine design: A novel bacterial adjuvant for peptide-specific ctl induction
20. Counting antigen-specific CD8 T cells: A reevaluation of bystander activation during viral infection
21. Vaccination of melanoma patients with petideor tumor lysate-pulsed dendritic cells
22. Design and evaluation of antigen-specific vaccination strategies against cancer
23. Longitudinal phenotypic analysis of human immunodeficiency virus type 1-specific cytotoxic T lymphocytes: Correlation with disease progression
24. T-cell repertoire diversity and clonal expansions in normal and clinical samples
25. Oligoclonality of tumor-infiltrating lymphocytes from human melanomas
26. A new era for cancer immunotherapy based on the genes that encode cancer antigens
27. Immunologic and therapeutic evaluation of synthetic peptide vaccine for the treatment of patients with metastatic melanoma
28. Control of viremia in simian immunodeficiency virus infection by CD8+ lymphocytes
29. Clonal expansion of T lymphocytes in human melanoma metastases after treatment with a hapten-modified autologous tumor vaccine
30. Analysis of TCR usage in human tumors: A new tool for assessing tumor-specific immune responses
31. Identification of human melanoma peptides recognized by class I restricted tumor infiltrating T lymphocytes
32. Tetramer-guided analysis of TCR beta-chain usage reveals a large repertoire of melan-A-specific CD8+ T cells in melanoma patients
33. Enhanced generation of specific tumor-reactive CTL in vitro by selected Melan-A/MART-1 immunodominant peptide analogues
34. Optimal activation of tumor-reactive T cells by selected antigenic peptide analogues
35. Junctional biases in the naive TCR repertoire control the CTL response to an immunodominant determinant of HSV-1
36. Oligoclonal expansions of CD8(+) T cells in chronic HIV infection are antigen specific
37. Identification of an MHC class I-restricted autoantigen in type 1 diabetes by screening an organ-specific cDNA library