Analysis of the α/β T-cell receptor repertoire by competitive and quantitative family-specific PCR with exogenous standards and high resolution fluorescence based CDR3 size imaging

Journal of Immunological Methods

Volumn 210, Issue 2, 1997, Pages 235-249

  Manfras, Burkhard J ^a   Rudert, William A ^b   Trucco, Massimo ^b   Boehm, Bernhard O ^b  

^a UNIVERSITY OF ULM   (Germany)

^b UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

Author keywords

CDR3 region; Competitive PCR; Quantitative PCR; T cell receptor

Indexed keywords

T LYMPHOCYTE RECEPTOR ALPHA CHAIN; T LYMPHOCYTE RECEPTOR BETA CHAIN;

ARTICLE; CELL SELECTION; CONTROLLED STUDY; HUMAN; HUMAN CELL; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; T LYMPHOCYTE RECEPTOR GENE; T LYMPHOCYTE SUBPOPULATION;

DNA PRIMERS; FLUORESCENCE; HUMANS; POLYMERASE CHAIN REACTION; RECEPTORS, ANTIGEN, T-CELL, ALPHA-BETA; REPRODUCIBILITY OF RESULTS;

EID: 0031590676     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(97)00197-X     Document Type: Article

References (35)

1. Arden B., Clark S.P., Kabelitz D., Mak T.W. Human T-cell receptor variable gene segment families. Immunogenetics. 42:1995;455.
2. Choi Y., Kotzin B., Herron L., Callahan L., Marrack P., Kappler J. Interaction of Staphylococcus aureus toxin 'Superantigens' with human T-cells. Proc. Natl. Acad. Sci. U.S.A. 86:1989;8941.
3. Chomcynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162:1987;156.
4. Chothia C., Boswell D.R., Lesk A.M. The outline structure of the T-cell alpha/beta receptor. EMBO J. 17:1988;3745.
5. Currier J.R., Yassai M., Robinson M.A., Gorski J. Molecular defects in TCRBV genes preclude thymic selection and limit the expressed TCR repertoire. J. Immunol. 157:1996;170.
6. Davies M.M., Bjorkman P.J. T-cell antigen receptor genes and T-cell recognition. Nature. 334:1988;395.
7. Dressel D., Brütt C.H., Manfras B., Zollner T.M., Wunderlich A., Böhm B.O., Boehnke W.-H. Alopecia areata but not androgenetic alopecia is characterized by a restricted and oligoclonal T-cell receptor-repertoire among infiltrating lymphocytes. J. Cutaneous Pathol. 24:1997;164.
8. Even J., Lim A., Puisieux I., Ferradini L., Dietrich P.Y., Toubert A., Hercend T., Triebel F., Pannetier C., Kourilsky P. T-cell repertoires in healthy and diseased human tissues analysed by T-cell receptor beta-chain CDR3 size determination: evidence for oligoclonal expansions in tumours and inflammatory diseases. Res. Immunol. 146:1995;65.
9. Fandrey J., Bunn H.F. In vivo and in vitro regulation of erythropoietin mRNA: measurement by competitive polymerase chain reaction. Blood. 81:1993;327.
10. Genevee C., Diu A., Nierat J., Caignard A., Dietrich P.Y., Ferradini L., Roman R.S., Triebel F., Hercend T. An experimentally validated panel of subfamily-specific oligonucleotide primers (V alpha 1-w29/V beta 1-w24) for the study of human T-cell receptor variable V gene segment usage by polymerase chain reaction. Eur. J. Immunol. 22:1992;1261.
11. Gorski J., Yassai M., Zhu X., Kissella B., Keever C., Flomenberg N. Circulating T-cell repertoire complexity in normal individuals and bone marrow recipients analyzed by CDR3 size spectratyping: correlation with immune status. J. Immunol. 152:1994;5109.
12. Hall B.L., Finn O.J. PCR-based analysis of the T-cell receptor V beta multigene family: experimental parameters affecting its validity. BioTechniques. 13:1992;248.
13. Li B., Sehajpal P., Khanna H., Vlassara H., Cerami A., Stenzel K.H., Suthanthiran M. Differential regulation of transforming growth factor beta and interleukin-2 genes in human T-cells: demonstration of novel competitor DNA constructs in quantitative polymerase chain reaction. J. Exp. Med. 174:1991;1259.
14. Loh E.Y., Elliott J.F., Cwirla S., Lanier L., Davies M.M. Polymerase chain reaction with single-sided specificity: analysis of T-cell receptor delta chain. Science. 243:1989;217.
15. Marguerie C., Lunardi C., So A. PCR-based analysis of the TCR repertoire in human autoimmune diseases. Immunol. Today. 13:1992;336.
16. Maslanka K., Piatek T., Gorski J., Yassai M., Gorski J. Molecular analysis of T-cell repertoires spectratypes generated by multiplex polymerase chain reaction and evaluated by radioactivity or fluorescence. Hum. Immunol. 44:1995;28.
17. McCulloch R.K., Chong C.S., Hurley D.M. A evaluation of competitor type and size for use in the determination of mRNA by competitive PCR. PCR Meth. Appl. 4:1995;219.
18. Meurer S., Acuto O., Hussey R., Hodgdon J., Fitzgerald K., Schlossman S., Reinherz E. Evidence for the T3-associated 90KD heterodimer as the T-cell antigen receptor. Nature. 303:1983;808.
19. Moss P.A., Rosenberg W.M., Bell J.I. The human T-cell receptor in health and disease. Annu. Rev. Immunol. 10:1992;71.
20. Naumov Y.N., Naumova E.N., Gorski J. CD4+ and CD8+ circulating alpha/beta T-cell repertoires are equally complex and are characterized by different levels of steady-state TCR expression. Hum. Immunol. 48:1996;52.
21. Oksenberg J.R., Stuart S., Begovich A.B., Bell R.B., Erlich H.A., Steinman L., Bernard C.C. Limited heterogeneity of rearranged T-cell receptor V alpha transcripts in brains of multiple sclerosis patients. Nature. 345:1990;344.
22. Pannetier C., Even J., Kourilsky P. T-cell repertoire diversity and clonal expansions in normal and clinical samples. Immunol. Today. 16:1995;176.
23. Quiros-Roldan E., Sottini A., Bettinardi A., Albertini A., Imberti L., Primi D. Different TCRBV genes generate biased patterns of V-D-J diversity in human T-cells. Immunogenetics. 41:1995;91.
24. Siebert P.D., Larrick J.W. PCR mimics: competitive DNA fragments for use as internal standards in quantitative PCR. BioTechniques. 14:1993;244.
25. Spinella, 1994.
26. Uematsu Y. A novel and rapid cloning method for the T-cell receptor variable region sequences. Immunogenetics. 34:1991;174.
27. Uhrberg M., Wernet P. Genetic influence on the shaping of the human T-cell receptor repertoire: quantitative assessment by competitive polymerase chain reaction. Scand. J. Immunol. 44:1996;173.
28. Wang A.M., Doyle M.V., Mark D.F. Quantitation of mRNA by the polymerase chain reaction. Proc. Natl. Acad. Sci. U.S.A. 86:1989;9717.
29. Wei S., Charmley P., Robinson M.A., Concannon P. The extend of the human germline T-cell receptor V beta gene segment repertoire. Immunogenetics. 40:1994;27.
30. Weidmann E., Whiteside T.L., Giorda R., Herberman R.B., Trucco M. The T-cell receptor V beta gene usage in tumor-infiltrating lymphocytes and blood of patients with hepatocellular carcinoma. Cancer Res. 52:1992;5913.
31. Weiss U., Manfras B.J., Terjung D., Eiermann T., Wölpl A., Löliger C., Kühnl P., Boehm B.O. In vitro stimulation with glutamic acid decarboxylase [GAD65] leads to an oligoclonal response of peripheral T-cells in an IDDM patient. Scand. J. Immunol. 42:1995;673.
32. Williams A.F., Strominger J.L., Bell J., Mak T.W., Kappler J., Marrack P., Arden B. Nomenclature for T-cell receptor (TCR) gene segments of the immune system: WHO-IUIS Nomenclature Sub-Committee on TCR Designation. Bull. World Health Org. 71:1993;113.
33. Wilson R.K., Lai E., Concannon P., Barth R.K., Hood L.E. Structure, organization and polymorphism of murine and human T-cell receptor alpha and beta chain gene families. Immunol. Rev. 101:1988;149.
34. Wucherpfennig K., Ota K., Endo N., Seidman J.G., Rosenzweig A., Weiner H.L., Hafler D.A. Shared human T-cell receptor V beta usage to immunodominant regions of myelin basic protein. Science. 284:1990;1016.
35. Zinkernagel R., Doherty P. Restriction of in vitro T-cell mediated cytotoxicity in lymphocytic chorimeningitis within a syngeneic or semi allogenic system. Nature. 248:1974;701.