Experimental determination of group flux control coefficients in metabolic networks

Biotechnology and Bioengineering

Volumn 58, Issue 2-3, 1998, Pages 149-153

  Simpson, Troy W ^a   Shimizu, Hiroshi ^a   Stephanopoulos, Gregory ^a  

^a Massachusetts Institute of Technology   (United States)

Author keywords

Corynebacterium glutamicum; Flux control coefficients; Metabolic Control Analysis; Metabolic engineering; Top down MCA

Indexed keywords

AMINO ACIDS; BACTERIA; BIOSYNTHESIS; CARBOXYLATION; METABOLISM; PHOSPHORUS COMPOUNDS; SALTS;

FLUX CONTROL COEFFICIENT; LYSINE; METABOLIC CONTROL ANALYSIS; METABOLIC ENGINEERING; PYRUVATE;

CELL CULTURE;

LYSINE; PHOSPHOENOLPYRUVATE; PYRUVIC ACID;

ARTICLE; BACTERIAL METABOLISM; BACTERIUM CULTURE; BIOENGINEERING; CARBOXYLATION; CORYNEBACTERIUM GLUTAMICUM; NONHUMAN;

BACTERIA (MICROORGANISMS); CORYNEBACTERIUM GLUTAMICUM; POSIBACTERIA;

EID: 0032485899     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19980420)58:2/3<149::AID-BIT6>3.0.CO;2-L     Document Type: Article

References (6)

1. Brown, G. C., Hafner, R. P., Brand, M. D. 1990. A "top-down" approach to the determination of control coefficients by metabolic control theory. Eur. J. Biochem. 188: 321-325.
2. Kacser, H., Burns, J. A. 1973. The control of flux. Symp. Soc. Exp. Biol. 27: 65-104.
3. 13C NMR spectroscopy and GC-MS. Appl. Microbiol. Biotechnol. 47: 430-440.
4. Small, J. R., Kacser, H. 1993a. Responses of metabolic systems to large changes in enzyme activities and effectors. I. The linear treatment of unbranched chains. Eur. J. Biochem. 213: 613-624.
5. Small, J. R., Kacser, H. 1993b. Responses of metabolic systems to large changes in enzyme activities and effectors. II. The linear treatment of branched pathways and metabolite concentrations. Assessment of the general non-linear case. Eur. J. Biochem. 213: 625-640.
6. Stephanopoulos, G., Simpson, T. W. 1997. Flux amplification in complex metabolic networks. Chem. Eng. Sci. 52: 2607-2627.