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The authors report the identification in a two-hybrid screen of a novel mTOR interactor raptor. Further extensive analysis of this interaction leads to a model in which mTOR and raptor constitutively interacts in a weak interaction that is required for mTOR signaling. Under nutrient-poor conditions, the binding is strengthened by an additional interaction, leading to mTOR inhibition.
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The authors report the functional association of the KCNQ1-KCNE1 potassium channel with the scaffolding protein Yotiao. Yotiao is required for the β-adrenergic-induced phosphorylation and activation of the channel by protein kinase A. Yotiao also docks the protein phosphatase 1 that dephosphorylates and inactivates the potassium channel.
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Marx S.O., Kurokawa J., Reiken S., Motoike H., D'Armiento J., Marks A.R., Kass R.S. Requirement of a macromolecular signaling complex for beta adrenergic receptor modulation of the KCNQ1-KCNE1 potassium channel. Science. 295:2002;496-499 The authors report the functional association of the KCNQ1-KCNE1 potassium channel with the scaffolding protein Yotiao. Yotiao is required for the β-adrenergic-induced phosphorylation and activation of the channel by protein kinase A. Yotiao also docks the protein phosphatase 1 that dephosphorylates and inactivates the potassium channel.
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The paper reports the stable and functional association of the chaperone protein Hsp70 with several histone class I deacetylase complexes. The authors propose that Hsp70 may be involved in nucleosome remodeling.
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