Enhanced synthesis of poly(3-hydroxybutyrate) in recombinant Escherichia coli by means of error-prone PCR mutagenesis, saturation mutagenesis, and in vitro recombination of the type II polyhydroxyalkanoate synthase gene

Journal of Biochemistry

Volumn 133, Issue 1, 2003, Pages 139-145

  Takase, Kazuma ^a   Taguchi, Seiichi ^a,b   Doi, Yoshiharu ^a,c  

^a RIKEN   (Japan)

^b MEIJI UNIVERSITY   (Japan)

^c TOKYO INSTITUTE OF TECHNOLOGY   (Japan)

Author keywords

In vitro enzyme evolution; P(3HB) synthesis; Pseudomonas sp. 61 3 PHA synthase; Recombination; Saturation mutagenesis

Indexed keywords

GENE PRODUCT; POLY(3 HYDROXYBUTYRIC ACID); POLYHYDROXYALKANOATE SYNTHASE II; POLYHYDROXYALKANOIC ACID; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ARTICLE; ESCHERICHIA COLI; GENE MUTATION; GENETIC RECOMBINATION; MOLECULAR EVOLUTION; NONHUMAN; POINT MUTATION; POLYMERASE CHAIN REACTION; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS;

ACYLTRANSFERASES; AMINO ACID SEQUENCE; CELL LINE; DIRECTED MOLECULAR EVOLUTION; DNA, RECOMBINANT; ESCHERICHIA COLI; HYDROXYBUTYRATES; MOLECULAR SEQUENCE DATA; MUTAGENESIS; PLASMIDS; POINT MUTATION; POLYESTERS; POLYMERASE CHAIN REACTION; RECOMBINATION, GENETIC; SEQUENCE ALIGNMENT;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA; PSEUDOMONAS; PSEUDOMONAS SP.; PSEUDOMONAS SP. 61-3;

EID: 0037284491     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/jb/mvg015     Document Type: Article

References (36)

1. Sudesh, K., Abe, H., and Doi, Y. (2000) Synthesis, structure and properties of polyhydroxyalkanoates: biological polyesters. Prog. Polym. Sci. 25, 1503-1555
2. Taguchi, S., Maehara, A., Takase, K., Nakahara, M., Nakamura, H., and Doi, Y. (2001) In vivo assay system of the polymerase as a key enzyme for PHA biosynthesis. RIKEN Rev. 42, 3-6
3. Taguchi, S., Nakamura, H., Hiraishi, T., Yamato, I., and Doi, Y. (2002) In vitro evolution of a polyhydroxybutyrate synthase by intragenic suppression-type mutagenesis. J. Biochem. 131, 801-806
4. Taguchi, S., Matsusaki, H., Matsumoto, K., Takase, K., Taguchi, K., and Doi, Y. (2002) Biosynthsis of biodegradable polyesters from renewable carbon sources by recombinant bacteria. Polymer Intern. 51, 899-906
5. Kichise, T., Taguchi, S., and Doi, Y. (2002) Enhanced accumulation and changed monomer composition in polyhydroxyalkanoate (PHA) copolyester by in vitro evolution of Aeromonas caviae PHA synthase. Appl. Environ. Microbiol. 68, 2411-2419
6. Taguchi, S., Maehara, A., Takase, K., Nakahara, M., Nakamura, H., and Doi, Y. (2001) Analysis of mutational effects of polyhydroxybutylate (PHB) polymerase on bacterial PHB accumulation using an in vivo assay system. FEMS Microbiol. Lett. 198, 65-71
7. Matsusaki, H., Manji, K., Taguchi, K., Kato, M., Fukui, T., and Doi, Y. (1998) Cloning and molecular analysis of the poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate-co-3-hydroxyalkanoate) biosynthesis genes in Pseudomonas sp. strain 61-3. J. Bacteriol. 180, 6459-6467
8. Matsumoto, K., Matsusaki, H., Taguchi, K., Seki, M., and Doi, Y. (2002) Isolation and characterization of polyhydroxyalkanoates inclusions and their associated proteins in Pseudomonas sp. 61-3. Biomacromolecules 3, 787-792
9. Yanisch-Perron, C., Vieira, C., and Messing, J. (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33, 103-119
10. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd ed. (Laboratory, C.S.H., ed.) Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
11. Matsusaki, H., Abe, H., Taguchi, K., Fukui, T., and Doi, Y. (2000) Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyalkanoates) by recombinant bacteria expressing the PHA synthesis gene phaC1 from Pseudomonas sp. 61-3. Appl. Microbial. Biotechnol. 53, 401-409
12. Taguchi, S., Kuwasako, K., Suenaga, A., Okada, M., and Momose, H. (2000) Functional mapping against Escherichia coli for the broad-spectrum antimicrobial peptide, thanatin, based on an in vivo monitoring assay system. J. Biochem. 128, 745-754
13. Spiekermann, P., Rehm, B.H.A., Kalscheuer, R., Baumeister, D., and Steinbüchel, A. (1999) A sensitive, viable-colony staining method using Nile red for direct screening of bacteria that accumulate polyhydroxyalkanoic acids and other lipid storage compounds. Arch. Microbiol. 171, 73-80
14. Karr, D.B., Waters, J.K., and Emerich, D.W. (1983) Analysis of poly-β-hydroxybutyrate in Rhizobium japonicum bacteroids by ion-exclusion high-pressure liquid chromatography and UV detection. Appl. Environ. Microbiol. 46, 1339-1344
15. +-translocating vacuolar ATPase from Enterococcus hirae. J. Biol. Chem. 272, 24885-24890
16. Kunchner, O., and Arnold, F.H. (1997) Directed evolution of enzyme catalysts. Trends Biotechnol. 15, 523-530
17. Amara, A.A., Steinbüchel, A., and Rehm, B.H.A. (2002) In vivo evolution of the Aeromonas punctata polyhydroxyalkanoate (PHA) synthase: isolated and characterization of modified PHA synthases with enhanced activity. Appl. Microbiol. Biotechnol. 59, 477-482
18. Tange, T., Taguchi, S., and Momose, H. (1994) Improvement of a useful enzyme (subtilisin BPN') by an experimental evolution system. Appl. Microbiol. Biotechnol. 41, 239-244
19. Kano, H., Taguchi, S., and Momose, H. (1997) Cold adaptation of a mesophilic serine protease, subtilisin, by in vitro random mutagenesis. Appl. Microbiol. Biotechnol. 47, 46-51
20. Taguchi, S., Ozaki, A., and Momose, H. (1998) Engineering of a cold-adapted protease by sequential random mutagenesis and a screening system. Appl. Environ. Microbiol. 64, 492-495
21. Taguchi, S., Ozaki, A., Nonaka, T., Mitsui, Y., and Momose, H. (1999) A cold-adapted protease engineered by experimental evolution system. J. Biochem. 126, 689-693
22. Taguchi, S., Komada, S., and Momose, H. (2000) The complete amino acid substitutions at position 131 that are positively involved in cold adaptation of subtilisin BPN'. Appl. Environ. Microbiol. 66, 1410-1415
23. Miyazaki, K. and Arnold, F.H. (1999) Exploring nonnatural evolutionary pathways by saturation mutagenesis: rapid improvement of protein function. J. Mol. Evol. 49, 716-720
24. Jia, Y., Yuan, W., Wodzinska, J., Park, C., Sinskey, A.J., and Stubbe, J. (2001) Mechanistic studies on class I polyhydroxybutyrate (PHB) synthase from Ralstonia eutropha: class I and III synthases share a similar catalytic mechanism. Biochemistry 40, 1011-1019
25. Steinbüchel, A. and Hein, S. (2001) Biochemical and molecular basis of microbial synthesis of polyhydroxyalkanoates in microorganisms. Adv. Biochem. Eng. Biotechnol. 71, 81-123
26. Peoples, O.P. and Sinskey, A.J. (1989) Poly-β-hydroxybutyrate biosynthesis in Alcaligenes eutrophus H16. Identification and characterization of the PHB polymerase gene (phbC). J. Biol. Chem. 264, 15298-15303
27. Schubert, P., Steinbüchel, A., and Schlegel, H.G. (1988) Cloning of the Alcaligenes eutrophus poly-β-hydroxybutyrate synthetic pathway and synthesis of PHB in Escherichia coli. J. Bacteriol. 170, 5837-5847
28. Slater, S.C., Voige, W.H., and Dennis, D.E. (1988) Cloning and expression in Escherichia coli of the Alcaligenes eutrophus H16 poly-β-hydroxybutyrate biosynthetic pathway. J. Bacteriol. 170, 4431-4436
29. Fukui, T. and Doi, Y. (1997) Cloning and analysis of the poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) biosynthesis genes of Aeromonas caviae. J. Bacteriol. 179, 4821-4830
30. Choi, J.-I., Lee, S.Y., and Han, K. (1998) Cloning of the Alcaligenes latus polyhydroxyalkanoate biosynthesis genes and use of these genes for enhanced production of poly(3-hydroxybutyrate) in Escherichia coli. Appl. Environ. Microbiol. 64, 4897-4903
31. Timm, A. and Steinbüchel, A. (1992) Cloning and molecular analysis of the poly(3-hydroxyalkanoic acid) gene locus of Pseudomonas aeruginosa PAO1. Eur. J. Biochem. 209, 15-30
32. Huisman, G.W., Wonink, E.W., Meima, R., Kazemier, B., Terpstra, P., and Witholt, B. (1991) Metabolism of poly(3-hydroxyalkanoates) (PHAs) by Pseudomonas oleovorans. J. Biol. Chem. 266, 2191-2198
33. Liebergesell, M. and Steinbüchel, A. (1992) Cloning and nucleotide sequences of genes relevant for biosynthesis of poly(3-hydroxybutyric acid) in Chromatium vinosum strain D. Eur. J. Biochem. 209, 135-150
34. Liebergesell, M. and Steinbüchel, A. (1993) Cloning and molecular analysis of the poly(3-hydroxybutyric acid) biosynthetic genes of Thiocystis violacea. Appl. Microbiol. Biotechnol. 38, 493-501
35. Kaneko, T., Sato, S., Kotani, H., Tanaka, A., Asamizu, E., Nakamura, Y., Miyajima, N., Hirosawa, M., Sugiura, M., Sasamoto, S., Kimura, T., Hosouchi, T., Matsuno, A., Muraki, A., Nakazaki, N., Naruo, K., Okumura, S., Shimpo, S., Takeuchi, C., Wada, T., Watanabe, A., Yamada, M., Yasuda, M., and Tabata, S. (1996) Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions. DNA Res. 3, 109-136
36. Hein, S., Tran, H., and Steinbüchel, A. (1998) Synechocystis sp. PCC6803 possesses a two-component polyhydroxyalkanoic acid synthase similar to that of anoxygenic purple sulfur bacteria. Arch. Microbiol. 170, 162-170