Analysis of mutational effects of a polyhydroxybutyrate (PHB) polymerase on bacterial PHB accumulation using an in vivo assay system

FEMS Microbiology Letters

Volumn 198, Issue 1, 2001, Pages 65-71

  Taguchi, Seiichi ^a   Maehara, Akira ^a   Takase, Kazuma ^a   Nakahara, Maki ^a   Nakamura, Hirofumi ^a   Doi, Yoshiharu ^a  

^a RIKEN   (Japan)

Author keywords

Fitness landscape; In vivo assay system; Polyhydroxybutyrate polymerase; Polymerase chain reaction random mutagenesis; Ralstonia eutropha; hydrolase fold

Indexed keywords

HYDROLASE; POLY(3 HYDROXYBUTYRIC ACID); POLY(3 HYDROXYBUTYRIC ACID) POLYMERASE; POLYESTER; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ARTICLE; ENZYME ACTIVITY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; IN VIVO STUDY; MUTAGENESIS; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN LOCALIZATION; STRAIN DIFFERENCE; WAUTERSIA EUTROPHA;

ACYLTRANSFERASES; AMINO ACID SUBSTITUTION; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CUPRIAVIDUS NECATOR; DNA MUTATIONAL ANALYSIS; ESCHERICHIA COLI; HYDROXYBUTYRATES; MUTAGENESIS; POLYESTERS; POLYMERASE CHAIN REACTION; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY;

EID: 0035917520     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(01)00125-2     Document Type: Article

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