Isolating PCR-quality DNA from human feces with a soil DNA kit

BioTechniques

Volumn 28, Issue 4, 2000, Pages 640-646

  Clement, Brian G ^a   Kitts, Christopher L ^a  

^a CALIFORNIA POLYTECHNIC STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA FRAGMENT;

ARTICLE; DNA DETERMINATION; DNA PURIFICATION; DNA REPLICATION; POLYMERASE CHAIN REACTION; REACTION ANALYSIS;

EID: 0034038056     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/00284bm06     Document Type: Article

References (13)

1. Al-Soud, W.A. and P. Rådström. 1998. Capacity of nine thermostable DNA polymerases to mediate DNA amplification in the presence of PCR-inhibiting samples. Appl. Env. Microbiol. 64:3740-3753.
2. Boom, R., C.J.A. Sol, M.M.M. Salimans, C.L. Jansen, P.M.E. Wertheim-van Dillen and J. van der Noordaa. 1990. Rapid and simple method for purification of nucleic acids. J. Clin. Microbiol. 25:495-503.
3. Clement, B.G., L.E. Kehl, K.L. DeBord and C.L. Kitts. 1998. Terminal restriction fragment patterns (TRFPs), a rapid, PCR-based method for the comparison of complex bacterial communities. J. Microbiol. Meth. 31:135-142.
4. Farrelly, V., F.A. Rainey and E. Stackebrandt 1995. Effect of genome size and rrn gene copy number on PCR amplification of 16S rRNA genes from a mixture of bacterial species. Appl. Env. Microbiol. 61:2798-2801.
5. Fogel, G.B., C.R. Collins, J. Li and C.F. Brunk. 1999. Prokaryotic genome size and SSU rDNA copy number estimation of microbial relative abundance from a mixed population. Microb. Ecol. 38:93-113.
6. Kepner, R.L., Jr. and J.R. Pratt. 1994. Use of fluorochromes for direct enumeration of total bacteria in environmental samples: past and present. Microbiol. Rev. 58:603-615.
7. Kok, R.G., A. De Waal, F. Schut, G.W. Welling, G. Weenk and K.J. Hellingwerf. 1996. Specific detection and analysis of a probiotic Bifidobacterium strain in infant feces. Appl. Env. Microbiol. 62:3668-3672.
8. Liu, W.T., T.L. Marsh, H. Cheng and L.J. Forney. 1997. Characterization of microbial diversity by determining terminal restriction fragment length polymorphisms of genes encoding 16S rRNA. Appl. Envir. Microbiol. 63:4516-4522.
9. Polz, M.F. and C.M. Cavanaugh. 1998. Bias in template-to-product ratios in multitemplate PCR. 1998. Appl. Env. Microbiol. 64:3724-3730.
10. Tannock, G.W. 1999. A fresh look at the intestinal microflora, p. 5-14. In G.W. Tannock (Ed.), Probiotics: A Critical Review. Horizon Scientific Press, Wymondham, England, UK.
11. Wang, R-F., W.W. Cao and C.E. Cerniglia. 1996. PCR detection and quantitation of predominant anaerobic bacteria in human and animal fecal samples. Appl. Env. Microbiol. 62:1242-1247.
12. Wilson, K.H. and R.B. Blitchlngton. 1996. Human colonic biota studied by ribosomal DNA sequence analysis. Appl. Env. Microbiol. 62:2273-2278.
13. Zoetendal, E.G., A.D.L. Akkermans and W.M. De Vos. 1998. Temperature gradient gel electrophoresis analysis of 16S rRNA from human fecal samples reveal stable and host-specific communities of active bacteria. Appl. Env. Microbiol. 64:3854-3859.