Suppression of a DNA double-strand break repair gene, Ku70, increases radio- and chemosensitivity in a human lung carcinoma cell line

DNA Repair

Volumn 1, Issue 4, 2002, Pages 299-310

  Omori, Shigenari ^a   Takiguchi, Yuichi ^a   Suda, Akira ^a   Sugimoto, Takaaki ^a   Miyazawa, Hiroshi ^a   Takiguchi, Yasuo ^a   Tanabe, Nobuhiro ^a   Tatsumi, Koichiro ^a   Kimura, Hiroshi ^b   Pardington, Paige E ^c   Chen, Fanqing ^d   Chen, David J ^d   Kuriyama, Takayuki ^a  

^a CHIBA UNIVERSITY   (Japan)

^b NARA MEDICAL UNIVERSITY   (Japan)

^c LOS ALAMOS NATIONAL LABORATORY   (United States)

^d LAWRENCE BERKELEY NATIONAL LABORATORY   (United States)

Author keywords

Antisense nucleic acid; DNA double strand break repair; DNA repair; Ionizing radiation; Ku70

Indexed keywords

BLEOMYCIN; CISPLATIN; CYTOTOXIC AGENT; DOUBLE STRANDED DNA; KU ANTIGEN; MESYLIC ACID METHYL ESTER; MITOMYCIN C; NUCLEIC ACID; PACLITAXEL; ANTINEOPLASTIC AGENT; CELL NUCLEUS ANTIGEN; COMPLEMENTARY DNA; DNA; DNA BINDING PROTEIN; HELICASE; PRIMER DNA; XRCC5 PROTEIN, HUMAN;

ARTICLE; CARCINOMA CELL; CHEMOSENSITIVITY; CONTROLLED STUDY; DNA REPAIR; DNA STRAND BREAKAGE; GENE CONSTRUCT; GENE REPRESSION; HUMAN; HUMAN CELL; LUNG CARCINOMA; PRIORITY JOURNAL; PROTEIN EXPRESSION; RADIOSENSITIVITY; SQUAMOUS CELL CARCINOMA; STATISTICAL SIGNIFICANCE; WILD TYPE; CELL CULTURE; CLONOGENIC ASSAY; COMPARATIVE STUDY; DNA DAMAGE; DRUG ANTAGONISM; DRUG EFFECT; GEL MOBILITY SHIFT ASSAY; GENETIC TRANSFECTION; GENETICS; IN VITRO STUDY; LUNG TUMOR; NORTHERN BLOTTING; RADIATION EXPOSURE; X RAY;

ANTIGENS, NUCLEAR; ANTINEOPLASTIC AGENTS; BLOTTING, NORTHERN; CARCINOMA, SQUAMOUS CELL; COLONY-FORMING UNITS ASSAY; DNA DAMAGE; DNA HELICASES; DNA PRIMERS; DNA REPAIR; DNA, ANTISENSE; DNA, NEOPLASM; DNA-BINDING PROTEINS; ELECTROPHORETIC MOBILITY SHIFT ASSAY; HUMANS; LUNG NEOPLASMS; TRANSFECTION; TUMOR CELLS, CULTURED; X-RAYS;

EID: 0037193539     PISSN: 15687864     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1568-7864(02)00006-X     Document Type: Article

References (42)

1. Gottlieb T.M., Jackson S.P. The DNA-dependent protein kinase: requirement for DNA ends and association with Ku antigen. Cell. 72:1993;131-142.
2. Dvir A., Peterson S.R., Knuth M.W., Lu H., Dynan W.S. Ku autoantigen is the regulatory component of a template-associated protein kinase that phosphorylates RNA polymerase II. Proc. Natl. Acad. Sci. U.S.A. 89:1992;11920-11924.
3. Hartley K.O., Gell D., Smith G.C.M., Zhang H., Divecha N., Connelly M.A., Admon A., Lees-Miller S.P., Anderson C.W., Jackson S.P. DNA-dependent protein kinase catalytic subunit: a relative of phosphatidylinositol 3-kinase and the Ataxia telangiectasia gene product. Cell. 82:1995;849-856.
4. Taccioli G.E., Gottlieb T.M., Blunt T., Priestley A., Demengeot J., Mizuta R., Lehmann A.R., Alt F.W., Jackson S.P., Jeggo P.A. Ku80: product of the XRCC5 gene and its role in DNA repair and V(D)J recombination. Science. 265:1994;1442-1445.
5. Peterson S.R., Kurimasa A., Oshimura M., Dynan W.S., Bradbury E.M., Chen D.J. Loss of the catalytic subunit of the DNA-dependent protein kinase in DNA double-strand-break-repair mutant mammalian cells. Proc. Natl. Acad. Sci. U.S.A. 92:1995;3171-3174.
6. Jeggo P.A. Idenfication of genes involved in repair of DNA double-strand breaks in mammalian cells. Radiat. Res. 150(5 Suppl.):1998;S80-S91.
7. Featherstone C., Jackson S.P. Ku, a DNA repair protein with multiple cellular functions? Mutat. Res. 434:1999;3-15.
8. Tuteja R., Tuteja N. Ku autoantigen: a multifunctional DNA binding protein. Crit. Rev. Biochem. Mol. Biol. 35:2000;1-33.
9. Mimori T., Ohosone Y., Hama N., Suwa A., Akizuki M., Homma M., Griffith A.J., Hardin J.A. Isolation and characterization of cDNA encoding the 80 kDa subunit protein of the human autoantigen Ku (p70/p80) recognized by autoantibodies from patients with scleroderma-polymyositis overlap syndrome. Proc. Natl. Acad. Sci. U.S.A. 87:1990;1777-1781.
10. Takiguchi Y., Kurimasa A., Chen F., Pardington P.E., Kuriyama T., Okinaka R.T., Moyzis R., Chen D.J. Genomic structure and chromosomal assignment of the mouse Ku70 gene. Genomics. 35:1996;129-135.
11. Dicroce P.A., Krontiris T.G. The BCL2 major breakpoint region is a sequence- and cell-cycle-specific binding site of the Ku antigen. Proc. Natl. Acad. Sci. U.S.A. 92:1995;10137-10141.
12. Giffin W., Torrance H., Rodda D.J., Prefontaine G.G., Pope L., Hache R.J.G. Sequence-specific DNA binding by Ku autoantigen and its effects on transcription. Nature. 380:1996;265-268.
13. Hsu H.L., Gilley D., Blackburn E.H., Chen D.J. Ku is associated with the telomere in mammals. Proc. Natl. Acad. Sci. U.S.A. 96:1999;12454-12458.
14. Li G.C., Ouyang H., Li X., Nagasawa H., Little J.B., Chen D.J., Ling C.C., Fuks Z., Cordon-Cardo C. Ku70: a candidate tumor suppressor gene for murine T cell lymphoma. Mol. Cell. 2:1998;1-8.
15. Smider V., Rathmell W.K., Lieber M.R., Chu G. Restoration of X-ray resistance and V(D)J recombination in mutant cells by Ku cDNA. Science. 266:1994;288-291.
16. Boubnov N.V., Hall K.T., Wills Z., Lee S.E., He D.M., Benjamin D.M., Pulaski C.R., Band H., Reeves W., Weaver D.T. Complementation of the ionizing radiation sensitivity, DNA end binding, and V(D)J recombination defects of double-strand break repair mutants by the p86 Ku autoantigen. Proc. Natl. Acad. Sci. U.S.A. 92:1995;890-894.
17. Nussenzweig A., Chen C., Soares V.C., Sanchez M., Sokol K., Nussenzweig M.C., Li G.C. Requirement for Ku80 in growth and immunoglobulin V(D)J recombination. Nature. 382:1996;551-555.
18. Zhu C., Bogue M.A., Lim D.S., Hasty P., Roth D.B. Ku86-deficient mice exhibit severe combined immunodeficiency and defective processing of V(D)J recombination intermediates. Cell. 86:1996;379-389.
19. Gu Y., Jin S., Gao Y., Weaver D.T., Alt F.W. Ku70-deficient embryonic stem cells have increased ionizing radiosensitivity, defective DNA end-binding activity, and inability to support V(D)J recombination. Proc. Natl. Acad. Sci. U.S.A. 94:1997;8076-8081.
20. Ouyang H., Nussenzweig A., Kurimasa A., Soares V.C., Li X., Cordon-Cardo C., Li W., Cheong N., Nussenzweig M., Iliakis G., Chen D.J., Li G.C. Ku70 is required for DNA repair but not for T cell antigen receptor gene recombination in vivo. J. Exp. Med. 186:1997;921-929.
21. Gu Y., Seidl K.J., Rathbun G.A., Zhu C., Manis J.P., Stoep N., Davidson L., Cheng H.L., Sekiguchi J.M., Alt F.W. Growth retardation and leaky SCID phenotype of Ku70-deficient mice. Immunity. 7:1997;653-665.
22. Lee S.E., Pulaski C.R., He D.M., Benjamin D.M., Voss M., Um J., Hendrickson E.A. Isolation of mammalian cell mutants that are X-ray sensitive, impaired in DNA double-strand break repair and defective for V(D)J recombination. Mutat. Res. 336:1995;279-291.
23. Calsou P., Muller C., Frit P., Salles B. Ku protein complex is involved in nucleotide excision repair of DNA. Comptes Rendus de 1 Academie des Sciences: Serie Iii, Sciences de la Vie. 319:1996;179-182.
24. Zdzienicka M.Z., Tran Q., van der Schans G.P., Simons J.W. Characterization of an X-ray-hypersensitive mutant of V79 Chinese hamster cells. Mutat. Res. 194:1997;239-249.
25. Johnston P.J., Stoppard E., Bryant P.E. Induction and distribution of damage in CHO-K1 and the X-ray-sensitive hamster cell line xrs5, measured by the cytochalasin-B-cytokinesis block micronucleus assay. Mutat. Res. 385:1997;1-12.
26. Carmichael J., Hickson I.D. Keynote address: mechanisms of cellular resistance to cytotoxic drugs and X-irradiation. Int. J. Radiat. Oncol. Biol. Phys. 20:1991;197-202.
27. Reeves W.H., Sthoeger Z.M. Molecular cloning of cDNA encoding the p70 (Ku) lupus autoantigen. J. Biol. Chem. 264:1989;5047-5052.
28. Niwa H., Yamamura K., Miyazaki J. Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene. 108:1991;193-200.
29. Ludwig D.L., Chen F., Peterson S.R., Nussenzweig A., Li G.C., Chen D.J. Ku80 gene expression is Sp1-dependent and sensitive to CpG methylation within a novel cis element. Gene. 199:1997;181-194.
30. Crooke S.T. Progress in antisense technology: the end of the beginning. Methods Enzymol. 313:2000;3-45.
31. Sburlati A.R., Manrow R.E., Berger S.L. Prothymosin α antisense oligomers inhibit myeloma cell division. Proc. Natl. Acad. Sci. U.S.A. 88:1991;253-257.
32. Akita H.D., Akie K., Hiroumi H., Kinoshita I., Kawakami Y., Murakami A. Inhibition of proliferation by L-myc antisense DNA for the translational initiation site in human small cell lung cancer. Cancer Res. 55:1995;1559-1564.
33. Errami A., Smider V., Rathmell W.K., He D.M., Hendrickson E.A., Zdzienicka M.Z., Chu G. Ku86 defines the genetic defect and restores X-ray resistance and V(D)J recombination to complementation group 5 hamster cell mutants. Mol. Cell. Biol. 16:1996;1519-1526.
34. Elkind M.M., Sutton H. X-ray damage and recovery in mammalian cells in culture. Nature. 184:1959;1293-1295.
35. Lehnert S., Rybka W.B., Suissa S., Giambattisto D. Radiation response of haematopoietic cell lines of human origin. Int. J. Radiat. Biol. 49:1986;423-431.
36. Byrnes R.W., Templin J., Sem D., Lyman S., Petering D.H. Intracellular DNA strand scission and growth inhibition of Ehrlich ascites tumor cells by bleomycins. Cancer Res. 50:1990;5275-5286.
37. Zdzienicka M.Z., van Wessel N., van der Schans G.P. A fourth complementation group among ionizing radiation-sensitive Chinese hamster cell mutants defective in DNA double-strand break repair. Radiat. Res. 131:1992;309-314.
38. Verhaegh G.W.C.T., Jongmans W., Morolli B., Jaspers N.G.J., van der Schans G.P., Lohman P.H.M., Zdzienicka M.Z. A novel type of X-ray-sensitive Chinese hamster cell mutant with radioresistant DNA synthesis and hampered DNA double-strand break repair. Mutat. Res. 337:1995;119-129.
39. Caldecott K., Jeggo P. Cross-sensitivity of γ-ray-sensitive hamster mutants to cross-linking agents. Mutat. Res. 255:1991;111-121.
40. Frit P., Canitrot Y., Muller C., Foray N., Calsou P., Marangoni E., Bourhis J., Salles B. Cross-resistance to ionizing radiation in a murine leukemic cell line resistant to cis-dichlorodiammineplatinum(II); role of Ku autoantigen. Mol. Pharm. 56:1999;141-146.
41. Turchi J.J., Henkels K. Human Ku autoantigen binds cisplatin-damaged DNA but fails to stimulate human DNA-activated protein kinase. J. Biol. Chem. 271:1996;13861-13867.
42. S. Hellman, Principles of cancer management: radiation therapy, in: V.T. Devita Jr., S. Hellman, S.A. Rosenberg (Eds.), Cancer - Principles and Practice of Oncology, 6th edition, Lippincott Williams & Wilkins, Philadelphia, 2001, pp. 265-288.