Transport and metabolism of opioid peptides across BeWo cells, an in vitro model of the placental barrier

International Journal of Pharmaceutics

Volumn 233, Issue 1-2, 2002, Pages 85-98

  Ampasavate, Chadarat ^a   Chandorkar, Gurudatt A ^a   Vande Velde, David G ^a   Stobaugh, John F ^a   Audus, Kenneth L ^a  

^a UNIVERSITY OF KANSAS   (United States)

Author keywords

BeWo; Metabolism; Opioid peptides; Permeability; Placenta

Indexed keywords

AMINOPEPTIDASE; ANALGESIC AGENT; DEXTRAN DERIVATIVE; OPIATE PEPTIDE; SUCROSE; SYNTHETIC PEPTIDE;

ARTICLE; BLOOD BRAIN BARRIER; BLOOD PLACENTA BARRIER; CANCER CELL CULTURE; CONTROLLED STUDY; DRUG DEGRADATION; DRUG METABOLISM; DRUG PENETRATION; DRUG TRANSPORT; HUMAN; HUMAN CELL; IN VITRO STUDY; MOLECULAR SIZE; MOLECULAR WEIGHT; PRIORITY JOURNAL;

ANALGESICS, OPIOID; ANIMALS; BIOLOGICAL TRANSPORT; CHORIOCARCINOMA; DRUG STABILITY; ENKEPHALIN, D-PENICILLAMINE (2,5)-; FEMALE; HUMANS; MOLECULAR WEIGHT; OPIOID PEPTIDES; PLACENTA; RATS; TUMOR CELLS, CULTURED; UTERINE NEOPLASMS;

EID: 0037148686     PISSN: 03785173     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-5173(01)00929-2     Document Type: Article

References (36)

1. Quantitative approaches to delineate paracellular diffusion in cultured epithelial cell monolayers
2. Factors regulating peptide passage across the placental barrier
3. pH-meteric log P10. Determination of liposomal membrane-water partition coefficients of ionizable drugs
4. Chromatographic indexes on immobilized artificial membranes for local anesthetics: Relationships with activity data on closed sodium channels
5. Retention mechanism for reversed-phase ion-pair liquid chromatography
6. Molecular charge effects on the protein permeability of the guinea pig placenta
7. Peptide transport and metabolism across the placenta
8. Superfused microcarrier cultures of choriocarcinoma cells: A dynamic model for studying transport of glucose and amino acids
9. Cerebral delta opioid receptors mediate analgesia but not the intestinal motility effects of intracerebroventricularly administered opioids
10. Enkephalin analog prodrugs: Assessment of in vitro conversion, enzyme cleavage characterization and blood-brain barrier permeability
11. Identification of two related pentapeptides from the brain with potent opiate agonist activity
12. Gestational and smoking effects on peptidase activity in the placenta
13. Fatty acid transport regulatory proteins in the developing rat placenta and in trophoblast cell cultures
14. Amino acid metabolism in the ovine fetus
15. Permeability properties of monolayers of the human trophoblast cell line BeWo
16. Endogenous opioid peptides: Multiple agonists and receptors
17. Human placental transport of oxytocin
18. Dipeptide transport in brush-border membrane vesicles (BBMV) prepared from human full-term placentae
19. Perinatal pharmacology: Placental transfer, fetal localization, and neonatal disposition of drugs
20. Placental transport
21. Conformationally constrained cyclic enkephalin analogs with pronounced delta opioid receptor agonist selectivity
22. Metabolism of dynorphin A 1-13 in human blood and plasma
23. Immobilized artificial membrane (IAM)-HPLC for partition studies of neutral and ionized acids and bases in comparison with the liposomal partition system
24. The establishment of a cell line of human hormone-synthesizing trophoblast cells in vitro
25. IAM chromatography: An in vitro screen for predicting drug membrane permeability
26. Functional expression of the plasma membrane serotonin transporter but not the vesicular monoamine transporter in human placental trophoblasts and choriocarcinoma cells
27. Development of delta opioid peptides as nonaddicting analgesics
28. Differential stereochemical requirements of mu vs. delta opioid receptors for ligand binding and signal transduction: Development of a class of potent and highly delta-selective peptide antagonists
29. Human placental dipeptidyl aminopeptidase III: Hydrolysis of enkephalins and its stimulation by cobaltous ion
30. Uptake of dehydroepiandrosterone-3-sulfate by isolated trophoblasts from human term placenta, JEG-3, BeWo, Jar, BHK cells, and BHK cells transfected with human sterylsulfatase-cDNA
31. Carrier-mediated transport of monocarboxylic acids in BeWo cell monolayers as a model of the human trophoblast
32. Carrier-mediated transport of valproic acid in BeWo cells, a human trophoblast cell line
33. Functional expression of P-glycoprotein in primary cultures of human cytotrophoblasts and BeWo cells
34. Rapid-gradient HPLC method for measuring drug interactions with immobilized artificial membrane: Comparison with other lipophilicity measures
35. Passage of a delta-opioid receptor selective enkephalin, [D-penicillamine2,5] enkephalin, across the blood-brain and the blood-cerebrospinal fluid barriers
36. Diffusion permeability of cyanocobalamin in human placenta