Functional expression of P-glycoprotein in primary cultures of human cytotrophoblasts and BeWo cells

Reproductive Toxicology

Volumn 14, Issue 3, 2000, Pages 217-224

  Utoguchi, Naoki ^a   Chandorkar, Gurudatt A ^a   Avery, Michael ^a   Audus, Kenneth L ^a  

^a UNIVERSITY OF KANSAS   (United States)

Author keywords

BeWo; Cell culture; Multidrug resistance; Placenta; Trophoblast

Indexed keywords

ABC TRANSPORTER; CALCEIN; CALCEIN AM; CYCLOSPORIN; CYCLOSPORIN A; DIPYRIDAMOLE; FLUORESCEIN; FLUORESCEIN DERIVATIVE; FLUORESCENT DYE; GLYCOPROTEIN P; MULTIDRUG RESISTANCE PROTEIN; MULTIDRUG RESISTANCE-ASSOCIATED PROTEIN; QUINIDINE; VERAPAMIL; VINBLASTINE;

ADULT; ARTICLE; CELL CULTURE; CHEMISTRY; CHORIOCARCINOMA; CONTROLLED STUDY; CULTURE MEDIUM; CYTOTROPHOBLAST; DRUG DISTRIBUTION; DRUG EFFECT; FEMALE; GENETICS; HUMAN; HUMAN CELL; IMMUNOBLOTTING; METABOLISM; MULTIDRUG RESISTANCE; PLACENTA; PREGNANCY; PROTEIN EXPRESSION; TROPHOBLAST; WESTERN BLOTTING;

ADULT; ATP-BINDING CASSETTE TRANSPORTERS; BLOTTING, WESTERN; CHORIOCARCINOMA; CULTURE MEDIA, CONDITIONED; CYCLOSPORINE; DIPYRIDAMOLE; DRUG RESISTANCE, MULTIPLE; FEMALE; FLUORESCEINS; FLUORESCENT DYES; HUMANS; MULTIDRUG RESISTANCE-ASSOCIATED PROTEINS; P-GLYCOPROTEIN; PREGNANCY; QUINIDINE; TROPHOBLASTS; TUMOR CELLS, CULTURED; VERAPAMIL; VINBLASTINE;

MAMMALIA;

EID: 0034046461     PISSN: 08906238     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0890-6238(00)00071-X     Document Type: Article

References (33)

1. Stulc J. Extracellular transport pathways in the haemochorial placenta. Placenta. 10:1989;113-119.
2. Ganapathy V., Prasad P., Ganapathy M., Leibach F. Drugs of abuse and placental transport. Adv Drug Del Rev. 38:1999;99-110.
3. Knipp G., Audus K.L., Soares M.J. Nutrient transport across the placenta. Adv Drug Del Rev. 38:1999;17-40.
4. Higgins C.F. ABC transporters from microorganisms to man . Annu Rev Cell Biol. 8:1992;67-113.
5. Chin K.V., Pastan I., Gottesman M.M. Function and regulation of the human multidrug resistance gene. Adv Cancer Res. 60:1993;157-180.
6. Sugawara I., Kataoka I., Morishita Y., et al. Tissue distribution of P-glycoprotein encoded by a multidrug-resistant gene as revealed by a monoclonal antibody, MRK 16. Cancer Res. 48:1988;1926-1929.
7. Thiebaut F., Tsuruo T., Hamada H., Gottesman M.M., Pastan I., Willingham M.C. Cellular localization of the multidrug-resistance gene product P-glycoprotein in normal human tissues. Proc Natl Acad Sci USA. 84:1987;7735-7738.
8. Thiebaut F., Tsuruo T., Hamada H., Gottesman M.M., Pastan I., Willingham M.C. Immunohistochemical localization in normal tissues of different epitopes in the multidrug transport protein P170 evidence for localization in brain capillaries and cross reactivity of one antibody with a muscle protein . J Histochem Cytochem. 37:1989;159-164.
9. Gottesman M.M., Pastan I., Ambudkar S.V. P-glycoprotein and multidrug resistance. Curr Opin Genet Develop. 6:1996;610-617.
10. Schinkel AH, Wagenaar E, Mol CAAM, van Deemter L. P-glycoprotein in the blood-brain barrier of mice influences the brain penetration and pharmacological activity of many drugs. J Clin Invest 1996;97:2517-24.
11. Kool M., de Haas M., Scheffer G.L., et al. Analysis of expression of cMOAT (MRP2), MRP3, MRP4, and MRP5, homologues of the multidrug resistance-associated protein gene (MRP1), in human cancer cell lines. Cancer Res. 57:1997;3537-3547.
12. Kool M., van der Linden P.A., de Haas M., Baas F., Borst P. Expression of human MRP6, a homologue of the multidrug resistance protein gene MRP1, in tissues and cancer cells. Cancer Res. 59:1999;175-182.
13. Nakamura Y., Ikeda S., Furukawa T., Sumizawa T., Tani A., Akiyama S., Nagata Y. Function of P-glycoprotein expressed in placenta and mole. Biochem Biophys Res Commun. 235:1997;849-853.
14. Lankas G.R., Wise L.D., Cartwright M.E., Pippert T., Umbenhauer D.R. Placental P-glycoprotein deficiency enhances susceptibility to chemically induced birth defects in mice. Reprod Toxicol. 12:1998;457-463.
15. Liu F., Soares M.J., Audus K.L. Permeability properties of monolayers of the human trophoblast cell line BeWo. Am J Physiol. 273:1997;C1596-C1604.
16. Chandorkar GA, Audus KL. Transport of opioid peptides across BeWo cell monolayers, a model of the placental barrier. Pharm Sci 1998;1:S-386.
17. Kenagy J., Liu F., Soares M.J., Audus K.L. Gestational and smoking effects on peptidase activity in the placenta. Peptides. 19:1998;1659-1666.
18. Utoguchi N, Magnusson M, Audus KL. Carrier-mediated transport of monocarboxylic acids in BeWo cell monolayers as a model of the human trophoblast. J Pharm Sci 1999;88:1288-92.
19. Utoguchi N, Audus KL. Carrier-mediated transport of valproic acid in BeWo cells, a human trophoblast cell line. Int J Pharm 2000;195:115-124.
20. Patillo R.A., Gey G.O. The establishment of a cell line of human hormone-synthesizing trophoblast cells in vitro. Cancer Res. 28:1968;1231-1237.
21. Huai-Yun H., Secrest D.T., Mark K.S., Carney D., Brandquist C., Elmquist W.F., Miller D.W. Expression of multidrug resistance-associated protein (MRP) in brain microvessel endothelial cells. Biochem Biophys Res Commun. 243:1998;816-820.
22. Seelig A. A general pattern for substrate recognition by P-glycoprotein. Eur J Biochem. 251:1998;252-261.
23. Dancis J. Placental physiology. In: Kretchmer N, Quilligan EJ, Johnson JD, editors. Prenatal and perinatal biology and medicine. Chur, Switzerland: Harwood Academic Publishers, 1987. p.1-33.
24. Mylona P., Glazier J., Greenwood S., Sides M., Sibley C. Expression of the cystic fibrosis (CF) and multidrug resistance (MDR1) genes during development and differentiation in the human placenta. Mol Hum Reprod. 2:1996;693-698.
25. Flens M.J., Zaman G.J., van der Valk P., et al. Tissue distribution of the multidrug resistance protein. Am J Pathol. 148:1996;1237-1247.
26. Legrand O., Simonin G., Perrot J., Zittoun R., Marie J. Pgp and MRP activities using calcein-AM are prognostic factors in adult acute myeloid leukemia patients. Blood. 91:1998;4480-4488.
27. Feller N., Broxterman H.J., Wahrer D.C.R., Pinedo H.M. ATP-dependent efflux of calcein by the multidrug resistance protein (MRP) no inhibition by intracellular glutathione depletion . FEBS Lett. 368:1995;385-388.
28. Rose J.M., Peckham S.L., Scism J.L., Audus K.L. Evaluation of the role of P-glycoprotein in ivermectin uptake by primary cultures of bovine brain microvessel endothelial cells. Neurochem Res. 23:1998;203-209.
29. Kokubu N., Cohen D., Watanabe T. Functional modulation of ATPase of P-glycoprotein by C219, a monoclonal antibody against P-glycoprotein. Biochem Biophys Res Commun. 230:1997;398-401.
30. Schinkel A.H., Smit J.J., van-Tellingen O., et al. Disruption of the mouse mdr1a P-glycoprotein gene leads to a deficiency in the blood-brain barrier and to increased sensitivity to drugs. Cell. 77:1994;491-502.
31. Allikmets R., Schriml L.M., Hutchinson A., Romano S.V., Dean M. A human placenta-specific ATP-binding cassette gene (ABCP) on chromosome 4q22 that is involved in multidrug resistance. Cancer Res. 58:1998;5337-5339.
32. Sugawara I., Akiyama S., Scheper R., Itoyama S. Lung resistance protein (LRP) expression in human normal tissues in comparison with that of MDR1 and MRP. Cancer Lett. 112:1997;23-31.
33. Shapiro A.B., Fox K., Lam P., Ling V. Stimulation of P-glycoprotein-mediated drug transport by prazosin and progesterone. Eur J Biochem. 259:1999;841-850.