Role of Rpn11 metalloprotease in deubiquitination and degradation by the 26S proteasome

Science

Volumn 298, Issue 5593, 2002, Pages 611-615

  Verma, Rati ^a   Aravind, L ^a   Oania, Robert ^a   McDonald, W Hayes ^a   Yates III, John R ^a   Koonin, Eugene V ^a   Deshaies, Raymond J ^a  

^a CALIFORNIA INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DEGRADATION; SUBSTRATES; YEAST;

MUTATION;

ENZYMES;

ADENOSINE TRIPHOSPHATASE; ADENOSINE TRIPHOSPHATE; DIMETHYL SULFOXIDE; DNA; EPOXOMICIN; METALLOPROTEINASE; PROTEASOME; UBIQUITIN;

BIOCHEMISTRY; PROTEIN;

ARTICLE; DNA REPAIR; ENZYME SUBSTRATE; EUKARYOTE; MASS SPECTROMETRY; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN DEGRADATION; YEAST;

ADENOSINE TRIPHOSPHATE; AMINO ACID MOTIFS; AMINO ACID SEQUENCE; BINDING SITES; CARBON-NITROGEN LYASES; CYSTEINE ENDOPEPTIDASES; DNA-BINDING PROTEINS; ENDOPEPTIDASES; FUNGAL PROTEINS; METALLOENDOPEPTIDASES; MOLECULAR SEQUENCE DATA; MULTIENZYME COMPLEXES; MUTATION; OLIGOPEPTIDES; PEPTIDE HYDROLASES; PROTEASOME ENDOPEPTIDASE COMPLEX; SACCHAROMYCES CEREVISIAE PROTEINS; TRANSCRIPTION FACTORS; UBIQUITINS; YEASTS; ZINC;

EUKARYOTA;

EID: 0037131243     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1075898     Document Type: Article

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34. This work was supported by Howard Hughes Medical Institute (R.V. and R.J.D.) and by a grant to University of Washington (W.H.M. and J.R.Y., UOW/RR11823-05-01). We are indebted to C. Crews for epoxomicin, K. Takeda and T. Toda for antiserum to pad1/Rpn11, T. Rinaldi for the MPR and mpr1-1 strains, M. Hochstrasser for ubp6Δ, C. H. Chung for Ubp6 antiserum, G. Alexandru and K. Nasmyth for the GAL-CLB2-HA3 strain, and the Deshaies lab members for critical comments.