Substrate promiscuity of an aminoglycoside antibiotic resistance enzyme via target mimicry

EMBO Journal

Volumn 21, Issue 10, 2002, Pages 2323-2331

  Fong, Desiree H ^a   Berghuis, Albert M ^a  

^a MCGILL UNIVERSITY   (Canada)

Author keywords

Antibiotic resistance; Crystal structure; Functional mimicry; Kinase; Multidrug binding

Indexed keywords

ADENOSINE DIPHOSPHATE; AMIKACIN; AMINOGLYCOSIDE ANTIBIOTIC AGENT; AMINOGLYCOSIDE KINASE III A; BACTERIAL ENZYME; BUTIROSIN; FRAMYCETIN; GENTAMICIN; ISEPAMICIN; KANAMYCIN A; KANAMYCIN B; LIVIDOMYCIN; PAROMOMYCIN; RIBOSTAMYCIN; RNA 16S; UNCLASSIFIED DRUG;

ANTIBIOTIC RESISTANCE; ARTICLE; CHEMICAL MODIFICATION; DRUG DESIGN; DRUG MISUSE; ENTEROCOCCUS; ENZYME STRUCTURE; ENZYME SUBSTRATE; GRAM POSITIVE BACTERIUM; PRIORITY JOURNAL; PROTEIN TARGETING; STAPHYLOCOCCUS;

CRYSTALLOGRAPHY, X-RAY; GRAM-POSITIVE BACTERIA; KANAMYCIN KINASE; MODELS, MOLECULAR; PROTEIN CONFORMATION; PROTEIN STRUCTURE, TERTIARY; RNA, RIBOSOMAL, 16S; SUBSTRATE SPECIFICITY;

BACTERIA (MICROORGANISMS); ENTEROCOCCUS; POSIBACTERIA; STAPHYLOCOCCUS;

EID: 0037093442     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/21.10.2323     Document Type: Article

References (44)

1. The structure of elongation factor G in complex with GDP: Conformational flexibility and nucleotide exchange
2. Crystallography and NMR system: A new software suite for macromolecular structure determination
3. Structural analyses of nucleotide binding to an aminoglycoside phosphotransferase
4. Functional insights from the structure of the 30S ribosomal subunit and its interactions with antibiotics
5. Changing patterns of infectious disease
6. Biologically important conformations of aminoglycoside antibiotics bound to an aminoglycoside 3′-phosphotransferase as determined by transferred nuclear Overhauser effect spectroscopy
7. Arrangement of substrates at the active site of an aminoglycoside antibiotic 3′-phosphotransferase as determined by NMR
8. Aminoglycoside antibiotics bound to aminoglycoside-detoxifying enzymes and RNA adopt similar conformations
9. The crystal structure of elongation factor G complexed with GDP at 2.7 Å resolution
10. Selection of an RNA molecule that mimics a major autoantigenic epitope of human insulin receptor
11. Structure of the A site of Escherichia coli 16S ribosomal RNA complexed with an aminoglycoside antibiotic
12. Binding of neomycin-class aminoglycoside antibiotics to the A-site of 16 S rRNA
13. Structure of an enzyme required for aminoglycoside antibiotic resistance reveals homology to eukaryotic protein kinases
14. Improved methods for building protein models in electron density maps and the location of errors in these models
15. RNA surfaces as functional mimetics of proteins
16. Aminoglycosides: Perspectives on mechanisms of action and resistance and strategies to counter resistance
17. The crystal structure of kanamycin
18. Imprinting through molecular mimicry: Protein synthesis
19. Broad spectrum aminoglycoside phosphotransferase type III from Enterococcus: Overexpression, purification and substrate specificity
20. Recognition of aminoglycoside antibiotics by enterococcal-staphylococcal aminoglycoside 3′-phosphotransferase type IIIa: Role of substrate amino groups
21. Aminoglycoside binding displaces a divalent metal ion in a tRNA-neomycin B complex
22. Aminoglycosides: Activity and resistance
23. Interaction of antibiotics with functional sites in 16S ribosomal RNA
24. Aminoglycoside phosphotransferase (3′)-IIIa [APH(3′)-IIIa]-bound conformation of the aminoglycoside lividomycin A characterized by NMR
25. Behind enemy lines
26. Phe, EF-Tu and a GTP analogue
27. Macromolecular mimicry
28. Crystal structures of Paracoccus denitrificans aromatic amino acid aminotransferase: A substrate recognition site constructed by rearrangement of hydrogen bond network
29. The active site of Paracoccus denitrificans aromatic amino acid aminotransferase has contrary properties: Flexibility and rigidity
30. Processing of X-ray diffraction data collected in oscillation mode
31. Conformational switch in the decoding region of 16S rRNA during aminoacyl-tRNA selection on the ribosome
32. Structural investigation of the antibiotic and ATP-binding sites in kanamycin nucleotidyltransferase
33. Recognition of structurally diverse substrates by type II 3-hydroxyacyl-CoA dehydrogenase (HADH II)/amyloid-β binding alcohol dehydrogenase (ABAD)
34. Genesis of methicillin-resistant Staphylococcus aureus (MRSA), how treatment of MRSA infections has selected for vancomycin-resistant Enterococcus faecium and the importance of antibiotic management and infection control
35. Structural mechanisms of QacR induction and multidrug recognition
36. Molecular genetics of aminoglycoside resistance genes and familial relationships of the aminoglycoside-modifying enzymes
37. The COOH terminus of aminoglycoside phosphotransferase (3′)-IIIa is critical for antibiotic recognition and resistance
38. Molecular mechanisms that confer antibacterial drug resistance
39. Crystal structure of a GCN5-related N-acetyltransferase: Serratia marcescens aminoglycoside 3-N-acetyltransferase
40. Aminoglycoside phosphotransferases: Proteins structure and mechanism
41. Aminoglycoside antibiotics. Structures, functions and resistance
42. Crystal structure of an aminoglycoside 6′-N-acetyltransferase: Defining the GCN5-related N-acetyltransferase superfamily fold
43. Structural origins of gentamicin antibiotic action
44. Structural basis of multidrug recognition by BmrR, a transcription activator of a multidrug transporter