Centromere/kinetochore localization of human centromere protein A (CENP-A) exogenously expressed as a fusion to green fluorescent protein

Cell Structure and Function

Volumn 25, Issue 4, 2000, Pages 253-261

  Sugimoto, K ^a   Fukuda, R ^a   Himeno, M ^a  

^a Osaka Prefecture University   (Japan)

Author keywords

Autoantigen; CENP A; CENP B; CENP C; Centromere; Kinetochore

Indexed keywords

ANTIBODY; AUTOANTIGEN; CENTROMERE PROTEIN A; CENTROMERE PROTEIN B; CENTROMERE PROTEIN C; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; NUCLEAR PROTEIN; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CELL CYCLE; CELL CYCLE G1 PHASE; CELL CYCLE S PHASE; CELL GROWTH; CELL LINE; CELL NUCLEUS; CENTROMERE; CONTROLLED STUDY; FLUORESCENCE; GROWTH INHIBITION; HETEROCHROMATIN; HUMAN; HUMAN CELL; INTERPHASE; MAMMAL CELL; METAPHASE; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; SCLERODERMA; STAINING;

ANIMALS; AUTOANTIGENS; CATTLE; CELL LINE; CELL NUCLEUS; CENTROMERE; CENTROMERE PROTEIN B; CHROMOSOMAL PROTEINS, NON-HISTONE; DNA-BINDING PROTEINS; GREEN FLUORESCENT PROTEINS; HETEROCHROMATIN; HUMANS; INTERPHASE; KINETOCHORES; LUMINESCENT PROTEINS; MICE; RECOMBINANT FUSION PROTEINS; SCYPHOZOA; SWINE; TUMOR CELLS, CULTURED;

AEQUOREA VICTORIA; ANIMALIA; MAMMALIA; SCLERODERMA; VICTORIA;

EID: 0034535720     PISSN: 03867196     EISSN: None     Source Type: Journal    
DOI: 10.1247/csf.25.253     Document Type: Article

References (33)

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2. Green fluorescent protein as a marker for gene expression
3. Characterization of the kinetochore binding domain of CENP-E reveals interactions with the kinetochore proteins CENP-F and hBUBR1
4. CENP-B: A major human centromere protein located beneath the kinetochore
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7. Microinjection of antibodies to centromere protein CENP-A arrests cells in interphase but does not prevent mitosis
8. Structure and dynamic organization of centromere/prekinetochores in the nucleus of mammalian cells
9. CENP-G: A new centromeric protein that is associated with the α-1 satellite DNA subfamily
10. Ultrastructure of the prophase kinetochore in culture cells of rat-kangaroo (Potorous tridactylis)
11. Early disruption of centromeric chromatin organization in centromere protein A (Cenpa) null mice
12. Cellular expression of human centromere protein C demonstrates a cyclic behaviour with highest abundance in the G1 phase
13. CENP-F is a protein of the nuclear matrix that assembles onto kinetochores at late G2 and is rapidly degraded after mitosis
14. A human centromere antigen (CENP-B) interacts with a short specific sequence in alphoid DNA, a human centromere satellite
15. The heterogeneity of anticentromere antibodies in immunoblotting analysis
16. Autoepitopes on autoantigen centromere protein-A (CENP-A) are restricted to the N-terminal region, which has no homology with histone H3
17. CENP-C, an autoantigen in scleroderma, is a component of the human inner kinetochore plate
18. Assembly of CENP-A into centromeric chromatin requires a cooperative array of nucelosomal DNA contact sites
19. Conservation of the centromere/kinetochore protein ZW10
20. A mutation in CSE4, an essential gene encoding a novel chromatin-associated protein in yeast, causes chromosome nondisjunction and cell cycle arrest at mitosis
21. Anti-helix-loop-helix antibodies: Discovery of autoantibodies that inhibit DNA binding activity of human centromere protein B (CENP-B)
22. An antigenic determinant on human centromere protein B (CENP-B) available for production of human-specific anticentromere antibodies in mouse
23. Human centromere protein C (CENP-C) is a DNA-binding protein which possesses a novel DNA-binding motif
24. Characterization of internal DNA-binding and C-terminal dimerization domains of human centromere/kinetochore autoantigen CENP-C in vitro: Role of DNA-binding and self-associating activities in kinetochore organization
25. Nucleotide specificity at the boundary and size requirement of the target sites recognized by human centromere protein B (CENP-B) in vitro
26. Visualization of prekinetochore locus on the centromeric region of highly extended chromatin fibers: Does kinetochore autoantigen CENP-C constitute a kinetochore organizing center?
27. Human CENP-A contains a histone H3 related histone fold domain that is required for targeting to the centromere
28. Identification of centromeric antigens in dicentric Robertsonian translocations: CENP-C and CENP-E are necessary components of functional centromeres
29. Chromatin containing CENP-A and α-satellite DNA is a major component of the inner kinetochore plate
30. Localization of anti-CENP antibodies and alphoid sequences in acentric heterochromatin in a breast cancer cell line
31. Immunolocalization of CENP-A suggests a distinct nucleosome structure at the inner kinetochore plate of active centromeres
32. HSα: Involvement of internal DNA-binding and C-terminal self-association domains in the formation of discrete dots in interphase nuclei
33. CENP-E is a putative kinetochore motor that accumulates just before mitosis