Functional gene screening in embryonic stem cells implicates Wnt antagonism in neural differentiation

Nature Biotechnology

Volumn 20, Issue 12, 2002, Pages 1240-1245

  Aubert, Jerôme ^a   Dunstan, Hannah ^a   Chambers, Ian ^a   Smith, Austin ^a  

^a UNIVERSITY OF EDINBURGH   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

MEMBRANE PROTEIN; ONCOPROTEIN; PROTEIN; SFRP2 PROTEIN, MOUSE; WNT PROTEIN; WNT1 PROTEIN; WNT1 PROTEIN, MOUSE; ZEBRAFISH PROTEIN; COMPLEMENTARY DNA; GENE PRODUCT; HIGH MOBILITY GROUP PROTEIN; LITHIUM CHLORIDE; MESSENGER RNA; PROTEIN SFRP2; RETINOIC ACID; SECRETED FRIZZLED RELATED PROTEIN 2; UNCLASSIFIED DRUG;

ANIMAL; ARTICLE; CELL CULTURE; CELL DIFFERENTIATION; CYTOLOGY; EMBRYO; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; GENETIC SCREENING; GENETICS; METABOLISM; METHODOLOGY; MOUSE; NERVE CELL; PHYSIOLOGY; PLASMID; STEM CELL; ANIMAL CELL; CELL LINEAGE; CELL SPECIFICITY; CONTROLLED STUDY; DNA LIBRARY; DNA SEQUENCE; EMBRYO CELL; EPISOME; EXPRESSED SEQUENCE TAG; IN SITU HYBRIDIZATION; IN VITRO STUDY; MOLECULAR CLONING; NERVE CELL DIFFERENTIATION; NERVOUS SYSTEM DEVELOPMENT; NEUROECTODERM; NONHUMAN; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

ANIMALS; CELL DIFFERENTIATION; CELLS, CULTURED; EMBRYO; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION, DEVELOPMENTAL; GENETIC SCREENING; MEMBRANE PROTEINS; MICE; NEURONS; PLASMIDS; PROTEINS; PROTO-ONCOGENE PROTEINS; STEM CELLS; WNT PROTEINS; WNT1 PROTEIN; ZEBRAFISH PROTEINS;

CELLS; CLONING; DNA; LITHIUM COMPOUNDS; NEUROLOGY;

NEURAL DIFFERENTIATION;

BIOTECHNOLOGY;

EID: 0036903270     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/nbt763     Document Type: Article

References (50)

1. Smith, A. in Stem Cell Biology (eds. Marshak, D. R., Gardner, R. L & Gottlieb, D.) 205-230 (Cold Spring Harbor Laboratory Press, New York, 2001).
2. Doetschman, T. C., Eistetter, H., Katz, M., Schmidt, W. & Kemler, R. The in vitro development of blastocyst-derived embryonic stem cell lines: Formation of visceral yolk sac, blood islands and myocardium. J. Embryol. Exp. Morphol. 87, 27-45 (1985).
3. Keller, G. M. In vitro differentiation of embryonic stem cells. Curr. Opin. Cell Biol. 7, 862-869 (1995).
4. Smith, A. G. Embryo-derived stem cells: Of mice and men. Ann. Rev. Cell Dev. Biol. 17, 435-462 (2001).
5. Fraichard, A. et al. In vitro differentiation of embryonic stem cells into glial cells and functional neurons. J. Cell Sci. 108, 3181-3188 (1995).
6. Bain, G., Kitchens, D., Yao, M., Huettner, J. E. & Gottlieb, D. I. Embryonic stem cells express neuronal properties in vitro. Dev. Biol. 168, 342-357 (1995).
7. Strubing, C. et al. Differentiation of pluripotent embryonic stem cells into the neuronal lineage in vitro gives rise to mature inhibitory and excitatory neurons. Mech. Dev. 53, 275-287 (1995).
8. Li, M., Pevny, L., Lovell-Badge, R. & Smith, A. Generation of purified neural precursors from embryonic stem cells by lineage selection. Curr. Biol. 8, 971-974 (1998).
9. Pevny, L. H. & Lovell-Badge, R. Sox genes find their feet. Curr. Opin. Genet. Dev. 7, 338-344 (1997).
10. Pevny, L. H., Sockanathan, S., Placzek, M. & Lovell-Badge, R. A role for Sox-1 in neural determination. Development 125, 1967-1978 (1998).
11. Niwa, H., Miyazaki, J. & Smith, A. G. Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells. Nat. Genet. 24, 372-376 (2000).
12. Diatchenko, L. et al. Suppression subtractive hybridization: A method for generating differentially regulated or tissue-specific cDNA probes and libraries. Proc. Natl. Acad. Sci. USA 93, 6025-6030 (1996).
13. Li, M. in Embryonic Stem Cells: Methods and Protocols Vol. 185 (ed. Turksen, K.) 205-215 (Humana Press, Totowa, NJ, 2001).
14. Oufad-Abdelghani, M. et al. Meis2, a novel mouse Pbx-related homeobox gene induced by retinoic acid during differentiation of P19 embryonal carcinoma cells. Dev. Dyn. 210, 173-183 (1997).
15. Leimeister, C., Bach, A. & Gessler, M. Developmental expression patterns of mouse sFRP genes encoding members of the secreted frizzled-related protein family. Mech. Dev. 75, 29-42 (1998).
16. Nagai, T. et al. The expression of the mouse Zic1, Zic2, and Zic3 gene suggests an essential role for Zic genes in body pattern formation. Dev. Biol. 182, 299-313 (1997).
17. Uwanogho, D. et al. Embryonic expression of the chicken Sox2, Sox3 and Sox11 genes suggests an interactive role in neuronal development. Mech. Dev. 49, 23-36 (1995).
18. Hargrave, M. et al. Expression of the Sox11 gene in mouse embryos suggests roles in neuronal maturation and epithelio-mesenchymal induction. Dev. Dyn. 210, 79-86 (1997).
19. Cheung, M., Abu-Elmagd, M., Clevers, H. & Scotting, P. J. Roles of Sox4 in central nervous system development. Mol. Brain Res. 79, 180-191 (2000).
20. Furushima, K., Murata, T., Matsuo, I. & Aizawa, S. A new murine zinc finger gene. Opr. Mech. Dev. 98, 161-164 (2000).
21. Caubit, X. et al. Vertebrate orthologues of the Drosophila region-specific patterning gene teashirt. Mech. Dev. 91, 445-448 (2000).
22. Cox, G. A., Mahaffey, C. L., Nystuen, A., Letts, V. A. & Frankel, W. N. The mouse fidgetin gene defines a new role for AAA family proteins in mammalian development. Nat. Genet. 26, 198-202 (2000).
23. Kiss, H. et al. A novel gene containing LIM domains (LIMD1) is located within the common eliminated region 1 (C3CER1) in 3p21.3. Hum. Genet. 105, 552-559 (1999).
24. Zhang, W. J. & Wu, J. Y. Sip-1, a novel RS domain-containing protein essential for pre-m RNA splicing. Mol. Cell. Biol. 18, 676-684 (1998).
25. Studler, J. M., Glowinski, J. & Levi-Strauss, M. An abundant mRNA of the embryonic brain persists at a high level in cerebellum, hippocampus and olfactory bulb during adulthood. Eur. J. Neurosci. 5, 614-623 (1993).
26. Rattner, A. et al. A family of secreted proteins contains homology to the cysteinerich ligand-binding domain of frizzled receptors. Proc. Natl. Acad. Sci. USA 94, 2859-2863 (1997).
27. Gassmann, M., Donoho, G. & Berg, P. Maintenance of an extrachromosomal plasmid vector in mouse embryonic stem cells. Proc. Natl. Acad. Sci. USA 92, 1292-1296 (1995).
28. Camenisch, G. et al. A polyoma-based episomal vector efficiently expresses exogenous genes in mouse embryonic stem cells. Nucleic Acids Res. 24, 3707-3713 (1996).
29. Niwa, H., Burdon, T., Chambers, I. & Smith, A. G. Self-renewal of pluripotent embryonic stem cells is mediated via activation of STAT3. Genes Dev. 12, 2048-2060 (1998).
30. Niwa, H., Masui, S., Chambers, I., Smith, A. G. & Miyazaki, J. Phenotypic complementation establishes requirements for specific POU domain and generic transactivation function of Oct-3/4 in embryonic stem cells. Mol. Cell. Biol. 22, 1526-1536 (2002).
31. Sommer, L., Ma, Q. & Anderson, D. J. Neurogenins, a novel family of atonal-related bHLH transcription factors, are putative mammalian neuronal determination genes that reveal progenitor cell heterogeneity in the developing CNS and PNS. Mol. Cell. Neurosci. 8, 221-241 (1996).
32. Walther, C. & Gruss, P. Pax6, a murine paired box gene, is expressed in the developing CNS. Development 113, 1435-1449 (1991).
33. Jostes, B., Walther, C. & Gruss, P. The murine paired box gene, Pax7, is expressed specifically during the development of the nervous and muscular system. Mech. Dev. 33, 27-37 (1990).
34. Conti, L. et al. Shc signaling in differentiating neural progenitor cells. Nat. Neurosci. 4, 579-586 (2001).
35. Lee, C. S., Buttitta, L. A., May, N. R., Kispert, A. & Fan, C. M. SHH-N upregulates Sfrp2 to mediate its competitive interaction with WNT1 and WNT4 in the somitic mesoderm. Development 127, 109-118 (2000).
36. Lescher, B., Haenig, B. & Kispert, A. sFRP-2 is a target of the Wnt-4 signaling pathway in the developing metanephric kidney. Dev. Dyn. 213, 440-451 (1998).
37. Ladher, R. K. et al. Cloning and expression of the Wnt antagonists Sfrp-2 and Frzb during chick development. Dev. Biol. 218, 183-198 (2000).
38. Melkonyan, H. S. et al. SARPs: A family of secreted apoptosis-related proteins. Proc. Natl. Acad. Sci. USA 94, 13636-13641 (1997).
39. Finch, P. W. et al. Purification and molecular cloning of a secreted, Frizzled-related antagonist of Wnt action. Proc. Natl. Acad. Sci. USA 94, 6770-6775 (1997).
40. Salic, A. N., Kroll, K. L., Evans, L. M. & Kirschner, M. W. Sizzled: A secreted Xwnt8 antagonist expressed in the ventral marginal zone of Xenopus embryos. Development 124, 4739-4748 (1997).
41. Chang, J. T. et al. Cloning and characterization of a secreted frizzled-related protein that is expressed by the retinal pigment epithelium. Hum. Mol. Genet. 8, 575-583 (1999).
42. Yamaguchi, T. P. Heads or tails: Wnts and anterior-posterior patterning. Curr. Biol. 11, R713-R724 (2001).
43. Easwaran, V., Pishvaian, M., Salimuddin & Byers, S. Cross- regulation of β-catenin-LEF/TCF and retinoid signaling pathways. Curr. Biol. 9, 1415-1418 (1999).
44. Yoshikawa, Y., Fujimori, T., McMahon, A. P. & Takada, S. Evidence that absence of Wnt-3a signaling promotes neuralization instead of paraxial mesoderm development in the mouse. Dev. Biol. 183, 234-242 (1997).
45. Galceran, J., Farinas, I., Depew, M. J., Clevers, H. & Grosschedl, R. Wnt3a(-/-)-like phenotype and limb deficiency in Lef1(-/-)Tcf1(-/-) mice. Genes Dev. 13, 709-717 (1999).
46. Yamaguchi, T. P., Takada, S., Yoshikawa, Y., Wu, N. & McMahon, A. P. T (Brachyury) is a direct target of Wnt3a during paraxial mesoderm specification. Genes Dev. 13, 3185-3190 (1999).
47. Roth, W. et al. Secreted Frizzled-related proteins inhibit motility and promote growth of human malignant glioma cells. Oncogene 19, 4210-4220 (2000).
48. Ellies, D. L., Church, V., Francis-West, P. & Lumsden, A. The WNT antagonist cSFRP2 modulates programmed cell death in the developing hindbrain. Development 127, 5285-5295 (2000).
49. Hooper, M. L., Hardy, K., Handyside, A., Hunter, S. & Monk, M. HPRT-deficient (Lesch-Nyhan) mouse embryos derived from germline colonization by cultured cells. Nature 326, 292-295 (1987).
50. Smith, A. G. Culture and differentiation of embryonic stem cells. J. Tiss. Cult. Methods 13, 89-94 (1991).