Genetic tags for labelling live cells: Gap junctions and beyond

Trends in Cell Biology

Volumn 12, Issue 9, 2002, Pages 399-404

  Falk, Matthias M ^a  

^a SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARSENIC DERIVATIVE; CYSTEINE; GREEN FLUORESCENT PROTEIN; ISOPROTEIN; RED FLUORESCENT PROTEIN; TRACER;

ARTICLE; AUTOFLUORESCENCE; BLEACHING; CYTOLOGY; GAP JUNCTION; HUMAN; MEMBRANE CHANNEL; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN MOTIF; PROTEIN SYNTHESIS; PROTEIN VARIANT; SOMATIC CELL GENETICS;

EID: 0036709318     PISSN: 09628924     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0962-8924(02)02343-7     Document Type: Review

References (38)

1. van Roessel P., Brand A.H. Imaging into the future: visualizing gene expression and protein interactions with fluorescent proteins. Nat. Cell. Biol. 4:2002;E15-E20.
2. Lippincott-Schwartz J., et al. Studying protein dynamics in living cells. Nat. Rev. Mol. Cell Biol. 2:2001;444-456.
3. Wouters F.S., et al. Imaging biochemistry inside cells. Trends Cell Biol. 11:2001;203-211.
4. Inouye S., Tsuji F.I. Aequorea green fluorescent protein. Expression of the gene and fluorescence characteristics of the recombinant protein. FEBS Lett. 341:1994;277-280.
5. Prasher D.C., et al. Primary structure of the Aequorea victoria green-fluorescent protein. Gene. 111:1992;229-233.
6. Ormo M., et al. Crystal structure of the Aequorea victoria green fluorescent protein. Science. 273:1996;1392-1395.
7. Yang F., et al. The molecular structure of green fluorescent protein. Nat. Biotechnol. 14:1996;1246-1251.
8. Heim R., Tsien R.Y. Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer. Curr. Biol. 6:1996;178-182.
9. Ellenberg J., et al. Dual-colour imaging with GFP variants. Trends Cell Biol. 9:1999;52-56.
10. Truong K., Ikura M. The use of FRET imaging microscopy to detect protein-protein interactions and protein conformational changes in vivo. Curr. Opin. Struct. Biol. 11:2001;573-578.
11. Hahn K., Toutchkine A. Live-cell fluorescent biosensors for activated signaling proteins. Curr. Opin. Cell Biol. 14:2002;167-172.
12. Jordan K., et al. Trafficking, assembly, and function of a connexin43-green fluorescent protein chimera in live mammalian cells. Mol. Biol. Cell. 10:1999;2033-2050.
13. Jordan K., et al. The origin of annular junctions: a mechanism of gap junction internalization. J. Cell Sci. 114:2001;763-773.
14. Falk M.M. Biosynthesis and structural composition of gap junction intercellular membrane channels. Eur. J. Cell Biol. 79:2000;564-574.
15. Falk M.M. Connexin-specific distribution within gap junctions revealed in living cells. J. Cell Sci. 113:2000;4109-4120.
16. Bukauskas F.F., et al. Clustering of connexin 43-enhanced green fluorescent protein gap junction channels and functional coupling in living cells. Proc. Nat. Acad. Sci. U. S. A. 97:2000;2556-2561.
17. Falk M.M., Lauf U. High resolution, fluorescence deconvolution microscopy and tagging with the autofluorescent tracers CFP, GFP, and YFP to study the structural composition of gap junctions in living cells. Microsc. Res. Tech. 52:2001;251-262.
18. Lopez P., et al. Distribution and dynamics of gap junction channels revealed in living cells. Cell Adhes. Commun. 8:2001;237-242.
19. Shen P., et al. Assembly, structure and degradation of gap junctions in living cells. Trends Cell Biol. 11:2001;183. (CD-ROM Supplement 'GFP in Motion 2').
20. Lauf, U. et al. Dynamic trafficking and delivery of connexons to the plasma membrane and accretion to gap junctions in living cells. Proc. Natl. Acad. Sci. U. S. A. (in press).
21. Gaietta G., et al. Multicolor and electron microscopic imaging of connexin trafficking. Science. 296:2002;503-507.
22. Weissleder R. A clearer vision for in vivo imaging. Nat. Biotechnol. 19:2001;316-317.
23. Matz M.V., et al. Fluorescent proteins from nonbioluminescent Anthozoa species. Nat. Biotechnol. 17:1999;969-973.
24. Wall M.A., et al. The structural basis for red fluorescence in the tetrameric GFP homolog DsRed. Nat. Struct. Biol. 7:2000;1133-1138.
25. Yarbrough D., et al. Refined crystal structure of DsRed, a red fluorescent protein from coral, at 2.0-Å resolution. Proc. Natl. Acad. Sci. U. S. A. 98:2001;462-467.
26. Baird G.S., et al. Biochemistry, mutagenesis, and oligomerization of DsRed, a red fluorescent protein from coral. Proc. Natl. Acad. Sci. U. S. A. 97:2000;11984-11989.
27. Jakobs S., et al. EFGP and DsRed expressing cultures of Escherichia coli imaged by confocal, two-photon and fluorescence lifetime microscopy. FEBS Lett. 479:2000;131-135.
28. Bevis B.J., Glick B.S. Rapidly maturing variants of the Discosoma red fluorescent protein (DsRed). Nat. Biotechnol. 20:2002;83-87.
29. Clontech Laboratories. Living Colors User Manual Vol II: Red Fluorescent Protein. 2002;Becton Dickinson.
30. Lauf U., et al. Expression of fluorescently tagged connexins: a novel approach to rescue function of oligomeric DsRed-tagged proteins. FEBS Lett. 498:2001;11-15.
31. Campbell R.E., et al. A monomeric red fluorescent protein. Proc. Natl. Acad. Sci. U. S. A. 99:2002;7877-7882.
32. Gurskaya N.G., et al. GFP-like chromoproteins as a source of far-red fluorescent proteins. FEBS Lett. 507:2001;16-20.
33. Li X., et al. Deletions of the Aequorea victoria green fluorescent protein define the minimal domain required for fluorescence. J. Biol. Chem. 272:1997;28545-28549.
34. Adams S.R., et al. New biarsenical ligands and tetracysteine motifs for protein labelling in vitro and in vivo: synthesis and biological applications. J. Am. Chem. Soc. 124:2002;6063-6076.
35. Griffin B.A., et al. Specific covalent labelling of recombinant protein molecules inside live cells. Science. 281:1998;269-272.
36. Griffin B.A., et al. Fluorescent labelling of recombinant proteins in living cells with FlAsH. Methods Enzymol. 327:2000;565-578.
37. Farinas J., Verkman A.S. Receptor-mediated targeting of fluorescent probes in living cells. J. Biol. Chem. 274:1999;7603-7606.
38. Niswender K.D., et al. Quantitative imaging of green fluorescent protein in cultured cells: comparison of microscopic techniques, use in fusion proteins and detection limits. J. Microsc. 180:1995;109-116.