Distribution and dynamics of gap junction channels revealed in living cells

Cell Communication and Adhesion

Volumn 8, Issue 4-6, 2001, Pages 237-242

  Lopez, Patricia ^a   Balicki, Danuta ^a,c   Buehler, Lukas K ^a   Falk, Matthias M ^a   Chen, Shu Chih ^b  

^a SCRIPPS RESEARCH INSTITUTE   (United States)

^b NORTHWEST HOSPITAL   (United States)

^c CENTRE HOSPITALIER DE L UNIVERSITÉ DE MONTRÉAL   (Canada)

Author keywords

Cyan fluorescent protein; Gap junctions; Green fluorescent protein; Life cell imaging; Protein dynamics; Yellow fluorescent protein

Indexed keywords

CELL MEMBRANE PROTEIN; FLUORESCENT DYE; GAP JUNCTION PROTEIN; GREEN FLUORESCENT PROTEIN; ISOPROTEIN; LIPID; PROTEIN SUBUNIT;

ARTICLE; AUTOFLUORESCENCE; CELL COMPOSITION; CELL FUNCTION; CELL JUNCTION; CELL MEMBRANE; CELL STRUCTURE; CHANNEL GATING; CLUSTER ANALYSIS; CONTROLLED STUDY; CYTOSKELETON; DIFFUSION; ENDOCYTOSIS; EXOCYTOSIS; GAP JUNCTION; HUMAN; HUMAN CELL; IMAGE ANALYSIS; MOLECULAR DYNAMICS; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN TRANSPORT; REGULATORY MECHANISM; SIGNAL TRANSDUCTION; STRUCTURE ANALYSIS;

EID: 0035754495     PISSN: 10615385     EISSN: None     Source Type: Journal    
DOI: 10.3109/15419060109080730     Document Type: Article

References (16)

1. Bretscher, M. S. 1996. Getting membrane flow and the cytoskeleton to cooperate in moving cells. Cell 15:601-606.
2. Brink, P. R., et al. 1997. Evidence for heteromeric gap junction channels formed from rat connexin43 and human connexin37. Am. J. Physiol. 273:C1386-1396.
3. Falk, M. M. 2000a. Connexin-specific distribution within gap junctions revealed in living cells. J. Cell Sci. 113:4109-4120.
4. Falk, M. M. 2000b. Biosynthesis and structural composition of gap junction intercellular membrane channels. Eur. J. Cell Biol. 79:564-574.
5. Falk, M. M., et al. 1997. Cell-free synthesis of connexins into functional gap junction membrane channels. EMBO J. 10:2703-2716.
6. Falk, M. M., and Lauf, U. 2000. High resolution, fluorescence deconvolution microscopy and tagging with the autofluorescent tracers CFP, GFP, and YFP to study the structure and function of gap junctions in living cells. Microsc. Res. Tech. 52:251-262.
7. Friend, D. S., and Gilula, N. B. 1972. Variations in tight and gap junctions in mammalian tissues. J. Cell Biol. 53:758-776.
8. Fujimoto, K., et al. 1997. Dynamics of connexins, E-cadherin and β-catenin on cell membranes during gap junction formation. J. Cell Sci. 110:311-322.
9. He, D. S., et al. 1999. Formation of heteromeric gap junction channels by connexins 40 and 43 in vascular smooth muscle cells. Proc. Natl. Acad Sci. USA 96:6495-6500.
10. Jiang, J. X., and Goodenough, D. A. 1996. Heteromeric connexons in lens gap junction channels. Proc. Natl. Acad. Sci. USA 93: 1287-1291.
11. Lauf, U., et al. 2001. Expression of fluorescently tagged connexins: a novel approach to rescue function of oligomeric DsRed-tagged proteins. FEBS Lett. 498:11-15.
12. Risek, B., et al. 1994. Developmental regulation and structural organization of connexins in epidermal gap junctions. Developmental Biol. 164:183-196.
13. Sheetz, M. P. 1993. Glycoprotein motility and dynamic domains in fluid plasma membranes. Annu. Rev. Biophys. Biomol. Struct. 22:417-431.
14. Shen, P., et al. 2001. Assembly, structure and degradation of gap junctions in living cells. GFP in Motion II. Trends Cell Biol. 11:183. (CD-ROM Supplement)
15. 2-connexin (connexin-26) can form heteromeric hemichannels. J. Biol. Chem. 270:6768-6772.
16. Steinman, R. M., et al. 1983. Endocytosis and the recycling of plasma membrane. J. Cell Biol. 96:1-27.