Purification and characterization of the fusion protein trypsin-streptavidin expressed in Escherichia coli

Journal of Protein Chemistry

Volumn 21, Issue 6, 2002, Pages 413-418

  Zhao, F ^a   Clare, D A ^a   Catignani, G L ^a   Swaisgood, Harold E ^a  

^a NORTH CAROLINA STATE UNIVERSITY   (United States)

Author keywords

Affinity column; Fusion protein; Protein expression; Protein purification; Streptavidin; Trypsin

Indexed keywords

ARGININE METHYL ESTER; BENZAMIDINE; HYBRID PROTEIN; ISOPROPYL THIOGALACTOSIDE; MONOMER; PROTEIN P39; PROTEIN P40; STREPTAVIDIN; TETRAMER; TRYPSIN; UNCLASSIFIED DRUG;

ARTICLE; BINDING AFFINITY; CELL FREE SYSTEM; CONTROLLED STUDY; ENZYME ACTIVITY; ESCHERICHIA COLI; GEL PERMEATION CHROMATOGRAPHY; MOLECULAR BIOLOGY; MOLECULAR SIZE; MOLECULAR WEIGHT; NONHUMAN; PROTEIN ANALYSIS; PROTEIN BLOOD LEVEL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; WESTERN BLOTTING; AFFINITY CHROMATOGRAPHY; CHEMISTRY; GENETICS; ISOLATION AND PURIFICATION; POLYACRYLAMIDE GEL ELECTROPHORESIS;

BLOTTING, WESTERN; CHROMATOGRAPHY, AFFINITY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; RECOMBINANT FUSION PROTEINS; STREPTAVIDIN; TRYPSIN;

EID: 0036704171     PISSN: 02778033     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1021134617137     Document Type: Article

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