Expression of recombinant human cytochrome P450 1A2 in Escherichia coli bacterial mutagenicity tester strain

Archives of Pharmacal Research

Volumn 21, Issue 3, 1998, Pages 305-309

  Chun, Young Jin ^a  

^a Chung Ang University   (South Korea)

Author keywords

2 Aminoanthracene; Escherichia coli WP2 uvrA; High level expression; Human cytochrome P450 1A2; MelQ

Indexed keywords

CYTOCHROME P450 1A2; MUTAGENIC AGENT; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; BIOSYNTHESIS; DRUG EFFECT; ESCHERICHIA COLI; GENETICS; HUMAN; IMMUNOLOGY; KINETICS; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; MUTAGEN TESTING; MUTAGENESIS; PLASMID; RAT;

AMINO ACID SEQUENCE; ANIMALS; CYTOCHROME P-450 CYP1A2; ESCHERICHIA COLI; HUMANS; KINETICS; MOLECULAR SEQUENCE DATA; MUTAGENESIS; MUTAGENICITY TESTS; MUTAGENS; PLASMIDS; RATS; RECOMBINANT PROTEINS;

EID: 0032082790     PISSN: 02536269     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02975292     Document Type: Article

References (22)

1. Barnes, H. J., Arlotto, M. P. and Waterman, M. R., Expression and enzymatic activity of recombinant cytochrome P450 17α-hydroxylase in Escherichia coli. Proc. Natl. Acad. Sci. USA, 88, 5597-5601 (1991).
2. Butler, M. A., Iwasaki, M., Guengerich, F. P. and Kadlubar, F. F., Human cytochrome P-450PA (P-450lA 2), the phenacetin O-deethylase, is primarily responsible for the hepatic 3-demethylation of caffeine and N-oxidation of carcinogenic arylamines. Proc. Natl. Acad. Sci. USA, 86, 7696-7700 (1989).
3. Butler, M. A., Lang, N. P., Young, J. F., Caporaso, N. E., Vineis, P., Hayes, R. B., Teitel, C. H., Massengill, J. P., Lawsen, M. F. and Kadlubar, F. F., Determination of CYP1A2 and NAT2 phenotypes in human populations by analysis of caffeine urinary metabolites. Pharmacogenetics, 2, 116-127 (1992).
4. Chun, Y. J., Shimada, T. and Guengerich, F. P., Construction of a human cytochrome P450 1A1: rat NADPH-cytochrome P450 reductase fusion protein cDNA and expression in Escherichia coli, purification, and catalytic properties of the enzyme in bacterial cells and after purification. Arch. Biochem. Biophys., 330, 48-58 (1996).
5. Dong, M. S., Bell, L. C., Guo, Z., Phillips, D. R., Blair, I. A. and Guengerich, F. P., Identification of retained N-formylmethionine in bacterial recombinant mammalian cytochrome P450 proteins with the N-terminal sequence MALLLAVFL...: roles of residues 3-5 in retention and membrane topology. Biochemistry, 35, 10031-10040 (1996).
6. Gillam, E. M. J., Baba, T., Kim, B. R., Ohmori, S. and Guengerich, F. P., Expression of modified human cytochrome P450 3A4 in Escherichia coli and purification and reconstitution of the enzyme. Arch. Biochem. Biophys., 305, 123-131 (1993).
7. + reversion in Escherichia coli. Mutat. Res., 38, 3-32 (1976).
8. Guengerich, F. P., Analysis and characterization of enzymes, In Hayes, A. W. (Ed.). Principles and Methods of Toxicology. Raven Press Ltd., New York, pp. 1259-1313, 1994.
9. Guengerich, F. P., Martin, M. V., Guo, Z. and Chun, Y.-J., Purification of recombinant human cytochrome P450 enzymes expressed in bacteria. Methods Enzymol., 272, 35-44 (1996).
10. Jenkins, C. M. and Waterman, M. R., Flavodoxin and NADPH-flavodoxin reductase from Escherichia coli support bovine cytochrome P450c17 hydroxylase activities. J. Biol. Chem., 269, 27401-27408 (1994).
11. Josephy, P. D., DeBruin, L. S., Lord, H. L., Oak, J. N., Evans, D. H., Guo, Z., Dong, M. S. and Guengerich, F. P., Bioactivation of aromatic amines by recombinant human cytochrome P4501A2 expressed in Ames tester strain bacteria:a substitute for activation by mammalian tissue preparations. Cancer Res., 55, 799-802 (1995).
12. Kudlubar, F. F., Butler, M. A., Kaderlik, K. R., Chou, H. C., and Lang, N. P., Polymorphisms for aromatic amine metabolism in humans: relevance for human carcinogenesis. Environ. Health Perspect, 98, 69-74 (1992).
13. Laemmli, U. K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685 (1970).
14. Maron, D. M. and Ames, B. N., Revised methods for the Salmonella mutagenicity test. Mutat. Res., 113, 173-215 (1983).
15. Omura, T. and Sato, R., The carbon monoxide-binding pigment of liver microsomes. I. Evidence for its hemoprotein nature. J. Biol. Chem., 239, 2370-2378 (1964).
16. Parikh, A. and Guengerich, F. P., Expression, purification, and characterization of a catalytically active human cytochrome P450 1A2: rat NADPH-cytochrome P450 reductase fusion protein. Protein Expr. Purif., 9, 346-354 (1997).
17. Parikh, A., Gillam, E. M. J. and Guengerich, F. P., Drug metabolism by Escherichia coli expressing human cytochromes P450. Nature Biotechnol., 15, 784-788 (1997).
18. Sandhu, P., Guo, Z., Baba, T., Martin, M. V., Tukey, R. H. and Guengerich, F. P., Expression of modified human cytochrome P450 1A2 in Escherichia coli: stabilization, purification, spectral characterization, and catalytic activities of the enzyme. Arch. Biochem. Biophys., 309, 168-177 (1994).
19. Sasardic, D., Boodis, A. R., Edwards, R. J. and Davies, D. S., A form of cytochrome P450 in man, orthologous to form d in the rat, catalyses the O-deethylation of phenacetin and is inducible by cigarette smoking. Br. J. Clin. Pharmacol., 26, 363-372 (1988).
20. Shet, M. S., Fisher, C. W., Holmans, P. L. and Estabrook, R. W., Human cytochrome P450 3A4: enzymatic properties of a purified recombinant fusion protein containing NADPH-P450 reductase. Proc. Natl. Acad. Sci. USA, 90, 11748-11752 (1993).
21. Thier, R., Pemble, S. E., Kramer, H., Taylor, J. B., Guengerich, F. P. and Ketterer, B., Human glutathione S-transferase T1-1 enhances mutagenicity of 1,2-dibromoethane, dibromomethane and 1,2,3,4-diepoxybutane in Salmonella typhimurium. Carcinogenesis, 17, 163-166 (1996).
22. Thier, R., Taylor, J. B., Pemble S. E., Humphreys W. G., Persmark M., Ketterer B. and Guengerich F. P., Expression of mammalian glutathione S-transferase 5-5 in Salmonella typhimurium TA1535 leads to base-pair mutations upon exposure to dihalomethanes. Proc. Natl. Acad. Sci. USA, 90, 8576-8580 (1993).