Cloning and expression of a streptavidin-lipase fusion gene in Escherchia coli and characterization of the immobilized fusion protein

Enzyme and Microbial Technology

Volumn 22, Issue 4, 1998, Pages 246-254

  Lee, P ^a   Swaisgood, H E ^a  

^a NORTH CAROLINA STATE UNIVERSITY   (United States)

Author keywords

Cloning; Escherichia coli; Fusion protein; Streptavidin lipase fusion gene; Transesterification

Indexed keywords

ADSORPTION; ANTIBODIES; CATALYST ACTIVITY; COLIFORM BACTERIA; DNA SEQUENCES; ENZYME IMMOBILIZATION; ESTERIFICATION; FATTY ACIDS; GENES; PARAFFINS; PURIFICATION;

BIOSELECTIVE ADSORPTION; OLEIC ACID; TRANSESTERIFICATION; TRICAPRYLIN;

CLONING;

ANTIBODY; HEXANE; HYBRID PROTEIN; IMMOBILIZED ENZYME; OLEIC ACID; RECOMBINANT DNA; SHORT CHAIN FATTY ACID; STREPTAVIDIN; TRIACYLGLYCEROL LIPASE; TRIOCTANOIN;

ADSORPTION; ARTICLE; CONTROLLED STUDY; DNA SEQUENCE; ENZYME ACTIVITY; ENZYME SPECIFICITY; ESCHERICHIA COLI; ESTERIFICATION; EXPRESSION VECTOR; GENE EXPRESSION; GENE FUSION; HYDROLYSIS; IMMUNOBLOTTING; MOLECULAR CLONING; MOLECULAR SIZE; NONHUMAN; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROMOTER REGION; PROTEIN PURIFICATION; PSEUDOMONAS FLUORESCENS;

ESCHERICHIA COLI; PSEUDOMONAS FLUORESCENS;

EID: 0032031269     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0141-0229(97)00174-9     Document Type: Article

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