Smad2 nucleocytoplasmic shuttling by nucleoporins CAN/Nup214 and Nup153 feeds TGFβ signaling complexes in the cytoplasm and nucleus

Molecular Cell

Volumn 10, Issue 2, 2002, Pages 271-282

  Xu, Lan ^a   Kang, Yibin ^a   Çöl, Seda ^a   Massagué, Joan ^a  

^a MEMORIAL SLOAN KETTERING CANCER CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOPLASM PROTEIN; NUCLEOPORIN; PROTEIN CAN; PROTEIN DERIVATIVE; PROTEIN FAST 1; PROTEIN NUP153; PROTEIN NUP214; SMAD ANCHOR FOR RECEPTOR ACTIVATION; SMAD PROTEIN; TRANSFORMING GROWTH FACTOR BETA RECEPTOR; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BINDING AFFINITY; CELL LINE; CELL NUCLEUS; CELL STRAIN 3T3; CELL STRAIN COS1; CONTROLLED STUDY; CYTOPLASM; HELA CELL; HUMAN; HUMAN CELL; HYDROPHOBICITY; MOLECULAR DYNAMICS; MOUSE; NONHUMAN; NUCLEAR IMPORT; NUCLEAR PORE COMPLEX; NUCLEOCYTOPLASMIC TRANSPORT; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN PHOSPHORYLATION; PROTEIN PROTEIN INTERACTION; SIGNAL TRANSDUCTION;

ANIMALIA;

EID: 0036670359     PISSN: 10972765     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1097-2765(02)00586-5     Document Type: Article

References (51)

1. Structural basis for the interaction between FxFG nucleoporin repeats and importin-β in nuclear trafficking
2. Gradient of increasing affinity of importin beta for nucleoporins along the pathway of nuclear import
3. Identification of an NTF2-related factor that binds Ran-GTP and regulates nuclear protein export
4. Nuclei from rat liver: Isolation method that combines purity with high yield
5. A transcriptional partner of MAD proteins in TGF-b signalling
6. Determinants of specificity in TGF-β signal transduction
7. E2F4/5 and p107 as Smad cofactors linking the TGFβ receptor to c-myc repression
8. Using viral species specificity to define a critical protein/RNA interaction surface
9. Identification and characterization of a nuclear pore complex protein
10. Transforming growth factor beta-inducible independent binding of SMAD to the Smad7 promoter
11. Nuclear localization signal-independent and importin/karyopherin-independent nuclear import of β-catenin
12. A conserved biogenesis pathway for nucleoporins: Proteolytic processing of a 186-kilodalton precursor generates Nup98 and the novel nucleoporin, Nup96
13. CRM1 is an export receptor for leucine-rich nuclear export signals
14. The human homologue of yeast CRM1 is in a dynamic subcomplex with CAN/Nup214 and a novel nuclear pore component Nup88
15. Structural basis for the recognition of a nucleoporin FG repeat by the NTF2-like domain of the TAP/p15 mRNA nuclear export factor
16. Transport between the cell nucleus and the cytoplasm
17. Intramolecular masking of the nuclear location signal and dimerization domain in the precursor for the p50 NF-κB subunit
18. The TGF β receptor activation process: An inhibitor- to substrate-binding switch
19. Distinct oligomeric state of SMAD proteins in the transforming growth factor-beta pathway
20. The human Tap protein is a nuclear mRNA export factor that contains novel RNA-binding and nucleocytoplasmic transport sequences
21. The Mex67p-mediated nuclear mRNA export pathway is conserved from yeast to human
22. Nucleocytoplasmic shuttling factors including Ran and CRM1 mediate nuclear export of NFAT In vitro
23. The human CAN protein, a putative oncogene product associated with myeloid leukemogenesis, is a nuclear pore complex protein that faces the cytoplasm
24. Leptomycin B inhibition of signal-mediated nuclear export by direct binding to CRM1
25. Transforming growth factor-beta induces nuclear import of Smad3 in an importin-beta1 and Ran-dependent manner
26. Dominant-negative mutants of importin-beta block multiple pathways of import and export through the nuclear pore complex
27. Ubiquitin-dependent degradation of TGF-beta-activated Smad2
28. The HIV-1 rev trans-activator acts through a structured target sequence to activate nuclear export of unspliced viral mRNA
29. TGFb signal transduction
30. Transcriptional control by the TGF-β/Smad signaling system
31. Nucleocytoplasmic transport: The soluble phase
32. A novel ubiquitin-like modification modulates the partitioning of the Ran-GTPase-activating protein RanGAP1 between the cytosol and the nuclear pore complex
33. Mammalian karyopherin alpha 1 beta and alpha 2 beta heterodimers: Alpha 1 or alpha 2 subunit binds nuclear localization signal and beta subunit interacts with peptide repeat-containing nucleoporins
34. Import and export of the nuclear protein import receptor transportin by a mechanism independent of GTP hydrolysis
35. Nup153 is an M9-containing nucleoporin with a novel Ran-binding domain
36. Transforming growth factor beta-independent shuttling of Smad4 between the cytoplasm and nucleus
37. Different Smad2 partners bind a common hydrophobic pocket in Smad2 via a defined proline-rich motif
38. Protein import into nuclei: Association and dissociation reactions involving transport substrate, transport factors, and nucleoporins
39. A nuclear pore complex protein that contains zinc finger motifs, binds DNA, and faces the nucleoplasm
40. SARA, a FYVE domain protein that recruits Smad2 to the TGFβ receptor
41. The nucleoporin nup153 plays a critical role in multiple types of nuclear export
42. G2 arrest and impaired nucleocytoplasmic transport in mouse embryos lacking the proto-oncogene CAN/Nup214
43. The translocation (6;9), associated with a specific subtype of acute myeloid leukemia, results in the fusion of two genes, dek and can, and the expression of a chimeric, leukemia-specific dek-can mRNA
44. The nucleoporin Nup153 is required for nuclear pore basket formation, nuclear pore complex anchoring and import of a subset of nuclear proteins
45. Regulation of intracellular dynamics of Smad4 by its leucine-rich nuclear export signal
46. Regulation of Smad activity
47. Structural basis of Smad2 recognition by the Smad anchor for receptor activation
48. Crystal structure of a phosphorylated Smad2. Recognition of phosphoserine by the MH2 domain and insights on Smad function in TGF-β signaling
49. Importin beta mediates nuclear translocation of Smad 3
50. The nuclear import function of Smad2 is masked by SARA and unmasked by TGFb-dependent phosphorylation
51. Intramolecular masking of nuclear import signal on NF-AT4 by casein kinase I and MEKK1