Fluorescence assay for the binding of ribonuclease A to the ribonuclease inhibitor protein

Analytical Biochemistry

Volumn 306, Issue 1, 2002, Pages 100-107

  Abel, Richele L ^a   Haigis, Marcia C ^a   Park, Chiwook ^a   Raines, Ronald T ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

Competition assay; Equilibrium dissociation constant; Fluorescence spectroscopy; Protein protein interaction; Ribonuclease A; Ribonuclease inhibitor

Indexed keywords

FLUORESCENCE; FLUORESCENCE SPECTROSCOPY; PROTEINS; RATE CONSTANTS;

COMPETITION ASSAYS; EQUILIBRIUM DISSOCIATION CONSTANT; PROTEIN-PROTEIN INTERACTIONS; RIBONUCLEASE A; RIBONUCLEASE INHIBITOR;

DISSOCIATION;

INHIBITOR PROTEIN; RIBONUCLEASE A; RIBONUCLEASE INHIBITOR PROTEIN; UNCLASSIFIED DRUG; ENZYME INHIBITOR; FLUORESCEIN; FLUORESCENT DYE; PANCREATIC RIBONUCLEASE;

ARTICLE; CANCER CELL; CONCENTRATION (PARAMETERS); CYTOTOXICITY; DISSOCIATION CONSTANT; ENZYME ACTIVITY; FLUORESCENCE; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; PROTEIN STRUCTURE; AMINO ACID SUBSTITUTION; BINDING COMPETITION; CHEMICAL STRUCTURE; CHEMISTRY; DRUG ANTAGONISM; ESCHERICHIA COLI; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; PROTEIN BINDING; PROTEIN CONFORMATION;

AMINO ACID SUBSTITUTION; BINDING, COMPETITIVE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ENZYME INHIBITORS; ESCHERICHIA COLI; FLUORESCEIN; FLUORESCENCE; FLUORESCENT DYES; KINETICS; MODELS, MOLECULAR; MOLECULAR STRUCTURE; PROTEIN BINDING; PROTEIN CONFORMATION; RIBONUCLEASE, PANCREATIC;

EID: 0036629204     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2002.5678     Document Type: Article

References (42)

1. Ribonuclease A
2. Quantitative analysis of the effect of salt concentration on enzymatic catalysis
3. The leucine-rich repeat: A versatile binding motif
4. Cytoplasmic ribonuclease inhibitor
5. Evolution of ribonuclease inhibitor by gene duplication
6. Mechanism of ribonuclease inhibition by ribonuclease inhibitor protein based on the crystal structure of its complex with ribonuclease A
7. Eosinophil-derived neurotoxin and human liver ribonuclease
8. Recombinant human pancreatic ribonuclease produced in E. coli: Importance of the amino-terminal sequence
9. Eosinophil cationic protein and eosinophil-derived neurotoxin
10. Human placental ribonuclease inhibitor abolishes both angiogenic and ribonucleolytic activities of angiogenin
11. Tight-binding inhibition of angiogenin and ribonuclease A by placental ribonuclease inhibitor
12. Protein chemical and kinetic characterization of recombinant porcine ribonuclease inhibitor expressed in Saccharomyces cerevisiae
13. Molecular evolution of the ribonuclease superfamily
14. Structure and action of mammalian ribonuclease (angiogenin) inhibitor
15. The angiogenins
16. Angiogenin, a potent mediator of angiogenesis
17. Ribonuclease A variants with potent cytotoxic activity
18. A ribonuclease A variant with low catalytic activity but potent cytotoxicity
19. Endowing human pancreatic ribonuclease with toxicity for cancer cells
20. Cancer chemotherapy - Ribonucleases to the rescue
21. Role of the N terminus in RNase A homologues: Differences in catalytic activity, ribonuclease inhibitor interaction and cytotoxicity
22. Tryptophan fluorescence as a probe of placental ribonuclease inhibitor binding of angiogenin
23. High-level soluble production and characterization of porcine ribonuclease inhibitor
24. Thiol-disulfide exchange of ribonuclease inhibitor bound to ribonuclease A
25. No role for pepstatin-A-sensitive acidic proteinase in reovirus infections of L or MDCK cells
26. A highly active immobilized ribonuclease
27. Engineering ribonuclease A: Production, purification, and characterization of wild-type enzyme and mutants at Gln11
28. Ribonuclease A: Revealing structure-function relationships with semi-synthesis
29. Sulfur shuffle: Modulating enzymatic activity by thiol-disulfide interchange
30. The correlation of ribonuclease activity with specific aspects of tertiary structure
31. The ribonucleolytic activity of angiogenin
32. Replacing a surface loop endows ribonuclease A with angiogenic activity
33. lac repressor binding to non-operator DNA: Detailed studies and a comparison of equilibrium and rate competition methods
34. Site-specific mutagenesis reveals differences in the structural bases for tight binding of RNase inhibitor to angiogenin and RNase A
35. Superadditive and subadditive effects of "hot spot" mutations within the interfaces of placental ribonuclease inhibitor with angiogenin and ribonuclease A
36. Analysis of the interactions of human ribonuclease inhibitor with angiogenin and ribonuclease A by mutagenesis: Importance of inhibitor residues inside versus outside the C-terminal "hot spot"
37. Kinetic mechanism of a partial folding reaction. 1. Properties of the reaction and effects of denaturants
38. A specific transition state for S-peptide combining with folded S-protein and then refolding