Application of the crypt isolation technique to the assessment of genetic alterations of colorectal carcinomas

Pathology International

Volumn 52, Issue 10, 2002, Pages 628-635

  Takahashi, Hiroshi ^a   Sugai, Tamotsu ^a   Habano, Wataru ^a   Nakamura, Shin Ichi ^a  

^a IWATE MEDICAL UNIVERSITY   (Japan)

Author keywords

APC; Colorectal cancer; Crypt isolation technique; DNA multiploidy; Loss of heterozygosity; p53

Indexed keywords

APC PROTEIN; PROTEIN P53;

ACCURACY; ALLELE; ANEUPLOIDY; ARTICLE; CELL POPULATION; CHROMOSOME ANALYSIS; CLINICAL ARTICLE; COLORECTAL CARCINOMA; CONTROLLED STUDY; DIPLOIDY; FEMALE; FLUORESCENCE; GENE MUTATION; GENETIC ANALYSIS; GENETIC ASSOCIATION; HUMAN; HUMAN TISSUE; INCIDENCE; ISOLATION PROCEDURE; MALE; METHODOLOGY; MICROSATELLITE INSTABILITY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; TUMOR CELL;

ADENOCARCINOMA, MUCINOUS; ADULT; AGED; AGED, 80 AND OVER; COLORECTAL NEOPLASMS; DNA MUTATIONAL ANALYSIS; DNA, NEOPLASM; FEMALE; FLOW CYTOMETRY; GENES, TUMOR SUPPRESSOR; HUMANS; INTESTINAL MUCOSA; LOSS OF HETEROZYGOSITY; MALE; MICROSATELLITE REPEATS; MIDDLE AGED; PLOIDIES; POLYMERASE CHAIN REACTION; SPECIMEN HANDLING;

EID: 0036436027     PISSN: 13205463     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1440-1827.2002.01395.x     Document Type: Article

References (32)

1. Fearon ER, Vogelstein B. A genetic model for colorectal tumorigenesis. Cell 1990; 61: 759-767.
2. Vogelstein B, Fearon ER, Hamilton SR et al. Genetic alterations during colorectal-tumor development. N. Eng. J. Med. 1988; 319: 525-532.
3. Thibodeau SN, Bren G, Schaid D. Microsatellite instability in cancer of the proximal colon. Science 1993; 260: 816-819.
4. Vogelstein B, Fearon ER, Kern SE et al. Allelotype of colorectal carcinomas. Science 1989; 244: 207-211.
5. Cawkwell L, Lewis FA, Quirke P. Frequency of allele loss of DCC, p53, RB1, WT1, NF1, NM23, and APC/MCC in colorectal cancer assayed by fluorescent multiple polymerase chain reaction. Br. J. Cancer 1994; 70: 813-818.
6. Habano W, Sugai T, Nakamura S, Yoshida T. A novel method for gene analysis of colorectal carcinomas using a crypt isolation technique. Lab. Invest. 1996; 74: 933-940.
7. Sugai T, Habano W, Nakamura S, Uesugi N, Sasou S, Itoh C. A unique method for mutation analysis of tumor suppressor genes in colorectal carcinomas using a crypt isolation technique. Arch. Pathol. Lab. Med. 2000; 124: 382-386.
8. Sugai T, Habano W, Nakamura S et al. Use of crypt isolation to determine loss of heterozygosity of multiple tumor suppressor genes in colorectal carcinoma. Pathol. Res. Pract. 2000; 196: 145-150.
9. Nakamura S, Goto J, Kitayama M, Kino I. Application of the crypt-isolation technique to flow-cytometric analysis of DNA content in colorectal neoplasms. Gastroenterology 2000; 106: 100-110.
10. Sugai T, Habano W, Nakamura S et al. Allelic losses of 17p, 5q and 18q loci in diploid and aneuploid populations of multiploid colorectal carcinomas. Hum. Pathol. 2000; 31: 925-930.
11. Sugai T, Habano W, Nakamura S et al. Genetic alterations in DNA diploid, aneuploid and multiploid colorectal carcinomas identified by the crypt isolation technique. Int. J. Cancer 2000; 88: 614-619.
12. Suzuki S, Mizuno M, Tomoda J, Ohmori M, Tsuji T. Flow cytometric analysis of the DNA content in colorectal adenomas with focal cancer. Gastroenterology 1995; 109: 1098-1104.
13. Hiddemann W, Von Bassewitz DB, Kleinemeier HJ et al. DNA stemline heterogeneity in colorectal cancer. Cancer 1985; 58: 258-263.
14. Giaretti W, Sciallero S, Bruno S, Geido E, Aste H, Di Vinci A. DNA flow cytometry of endoscopically examined colorectal adenomas and adenocarcinomas. Cytometry 1988; 9: 238-244.
15. Japanese Society for Cancer of the Colon and Rectum. Japanese Classification of Colorectal Carcinoma. Kanehara, Tokyo, 1997; 30-63.
16. Turnbull RB, Kyle K, Watson FR, Spratt J. Cancer of the colon; the influence of the no-touch isolation technique on survival rates. Ann. Surg. 1967; 166: 420-427.
17. Sugai T, Nakamura S, Habano W et al. Analysis of subclonal expansion of colorectal carcinomas using flow cytometry. Virchows Arch. 1999; 434: 437-441.
18. Halling KC, Harper J, Moskaluk CA et al. Origin of microsatellite instability in gastric cancer. Am. J. Pathol. 1999; 155: 205-211.
19. Grius NA, Abelin ECA, Bardoel AJF, Devilee P, Frants RR, Cornelisse CJ. PCR-based microsatellite polymorphism in the detection of loss of heterozygosity in fresh and archival tumor tissue. Br. J. Cancer 1993; 68: 308-313.
20. Cavenee WK, Dryja TP, Phillips RA et al. Expression of recessive alleles by chromosomal mechanism in retinoblastoma. Science 1983; 305: 779-784.
21. Connolly KC, Gabra H, Millwater CJ et al. Identification of a region of frequent loss of heterozygosity at 11q,24 in colorectal cancer. Cancer Res. 1999; 59: 2806-2809.
22. Nowell PC. Mechanism of tumor progression. Cancer Res. 1986; 46: 2203-2207.
23. Dietmaier W, Wallinger S, Bocker T, Kullmann F, Fishel R, Ruchoff J. Diagnostic microsatellite instability: Definition and correlation with mismatch repair protein expression. Cancer Res. 1997; 57: 4749-4756.
24. Ruchoff J, Bocker T, Schlegel J, Stumm G, Hofstaedter F. Microsatellite instability: New aspects in the carcinogenesis of colorectal carcinoma. Virchows Arch. 1995; 429: 215-222.
25. Kinzler KW, Vogelstein B. Lessons from hereditary colorectal cancer. Cell 1996; 87: 159-170.
26. Habano W, Sugai T, Nakamura S. Mismatch repair deficiency leads to a unique mode of colorectal tumorigenesis characterized by intratumoral heterogeneity. Oncogene 1998; 16: 1259-1265.
27. Jass JR, Do K-A, Simms LA et al. Morphology of sporadic colorectal cancer with DNA replication errors. Gut 1998; 42: 673-679.
28. Fujiwara T, Stolker JM, Watanabe T et al. Accumulated clonal genetic alterations in familial and sporadic colorectal carcinomas with widespread instability in microsatellite sequences. Am. J. Pathol. 1998; 153: 1063-1078.
29. Loda M. Polymerase chain reaction-based methods for the detection of mutations in oncogenes and tumor suppressor genes. Hum. Pathol. 1994; 25: 564-571.
30. Rowan AJ, Laulum H, Ilyas M et al. APC mutations in sporadic colorectal tumors: A mutational 'hotspot' and interdependence of the 'two hits'. Proc. Natl Acad. Sci. USA 2000; 97: 3352-3357.
31. Sugai T, Habano W, Uesugi N et al. Frequent allelic imbalance at the ATM locus in DNA multiploid colorectal carcinomas. Oncogene 2001; 20: 6095-6101.
32. Sugai T, Habano W, Nakamura S et al. Analysis of Ki-ras gene mutations associated with DNA diploid, aneuploid and multiploid colorectal carcinomas using a crypt isolation technique. Cytometry 2001; 46: 345-350.