Use of crypt isolation to determine loss of heterozygosity of multiple tumor suppressor genes in colorectal carcinoma

Pathology Research and Practice

Volumn 196, Issue 3, 2000, Pages 145-150

  Sugai, Tamotsu ^a   Habano, Wataru ^a   Nakamura, Shin Ichi ^a   Yoshida, Toru ^a   Uesugi, Noriyuki ^a   Sasou, Shunichi ^a   Itoh, Chuichi ^a   Katoh, Ryouhei ^b  

^a IWATE MEDICAL UNIVERSITY   (Japan)

^b UNIVERSITY OF YAMANASHI   (Japan)

Author keywords

Colorectal carcinoma; Crypt isolation technique; Loss of heterozygosity; Multiple tumor suppressor genes

Indexed keywords

ADULT; AGED; ALLELISM; ARTICLE; COLORECTAL CARCINOMA; CRYPT CELL; DNA DETERMINATION; FEMALE; GENE DELETION; GENE FREQUENCY; GENE LOCUS; GENE LOSS; HETEROZYGOSITY LOSS; HUMAN; HUMAN TISSUE; MALE; POLYMERASE CHAIN REACTION; TUMOR SUPPRESSOR GENE;

EID: 0034057134     PISSN: 03440338     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0344-0338(00)80094-1     Document Type: Article

References (26)

1. Arai T, Kino I (1989) Morphometrical and cell kinetic studies of normal human colorectal mucosa: Comparison between the proximal and the distal large intestine. Acta Pathol Jpn 39: 725-730
2. Boynton RF, Bount PL, Yin J, Brown VL, Huang Y, Tong Y, McDaniel T, Newkirk C, Resau JH, Raskind WH, Haggitt RC, Reid BJ, Meltzer SJ (1992) Loss of heterozygosity involving the APC and MCC genetic loci occurs in the majority of human esophageal cancers. Proc Natl Acad Sci USA 89; 3385-3388
3. Cawkwell L, Bell SM, Lewis FA, Dixon MF, Taylor GR, Quirke P (1993) Rapid detection of allelic loss in colorectal tumours using microsatellites and fluorescent DNA technology. Br J Cancer 67: 1262-1267
4. Cawkwell L, Lewis FA, Quirke P (1994) Frequency of allele loss of DCC, p53, RB1, WT1, NF1, NM23 and APC/MCC in colorectal cancer assayed by fluorescent multiple polymerase chain reaction. Br J Cancer 70: 813-818
5. Cohn KH, Wang FS, Desoto-Lapaix F, Solomon WB, Patterson LG, Arnold MR, Weimer J, Feldman JG, Levy AT, Leone A, Steeg PS (1991) Association of nm23 H-1 allelic deletions with distant metastases in colorectal carcinomas. Lancet 328: 722-724
6. Deng GR, Lu, Chen SM, Miao J, Lu G, Li H, Cai H, Xu Z, Zheng F, Liu P (1987) Activated c-Ha-ras oncogene with a guanine to thymine transversion at the twelfth codon in a human stomach cell line. Cancer Res 47: 3195-3196
7. Fearon ER, Vogelstein, B (1990) A genetic model for colorectal tumorgenesis. Cell 61: 759-767
8. Fujita K, Ohuchi N, Yao T, Okumura M, Fukushima Y, Kanakura Y, Kitamura Y, Fujita J (1987) Frequent overexpression, but not activation by point mutation of ras gene in primary human gastric cancers. Gastroenterology 93: 1339-1345
9. Habano W, Sugai T, Nakamura S. Yoshida T (1996) A novel method for gene analysis of colorectal carcinomas using a crypt isolation technique. Lab Invest 74: 933-840
10. Japanese Society for Cancer of the Colon and Rectum (1997) Japanese Classification of Colorectal Carcinoma. First ed, Kanehara Co., Tokyo, pp 30-63
11. Jones MH, Nakamura Y (1992) Detection of loss of heterozygosity at the human TP53 locus using a dinucleotide polymorphism. Genes Chromosom Cancer 5: 89-90
12. Leone A, McBride OW, Weston A, Wang MG, Anglard P, Cropp CS, Goepel JR, Lidereau R, Callahan R, Linehan WM, Rees RC, Harris CC, Liotta LA, Steeg PS (1991) Somatic allelic deletion of nm23 in human cancer. Cancer Res 51: 2490-2493
13. Meling GI, Lothe RA, Børresen AL, Graue C, Hauge S, Clausen OPF, Rognum TO (1993) The TP53 tumor suppressor gene in colorectal carcinomas. 1. Genetic alterations on chromosome 17. Br J Cancer 67: 88-92
14. Mies C (1994) Molecular biological analysis of paraffin-embedded tissues. Hum Pathol 23: 555-560
15. Nakamura S, Goto J, Kitayama M, Kino I (1994) Application of the crypt-isolation technique to flow-cytometric analysis of DNA content in colorectal neoplasms. Gastroenterology 106: 100-107
16. Neuman LW, Wasylyshyn ML, Jacoby R, Erroi F, Angriman I, Montag A, Brasitus T, Michelassi F, Westbrook CA (1991) Evidence for a common molecular pathogenesis in colorectal, gastric, and pancreatic cancer. Genes Chromosom Cancer 3: 468-476
17. Nowel PC (1976) The clonal evolution of tumor cell populations. Science 194: 23-28
18. Rustgi AK, Xu L, Pinney D, Sterner C, Beauchamp R, Schmidt S, Gusella JF, Ramesh V (1995) Neurofibromatosis 2 gene in human colorectal cancer. Cancer Genet Cytogenet 84: 24-26
19. Sainz J, Nechiporuk A, Kim UJ, Simon MI, Pulst SM (1993) CA-repeat polymorphism at the D22S430 locus adjacent to NF2. Hum Mol Genet 2: 2203
20. Tamura G, Kihana T, Nomura M, Terada M, Sugimura T, Hirohashi S (1991) Detection of frequent p53 mutation in primary gastric cancer by cell sorting and polymerase chain reaction-single strand conformation polymorphism analysis. Cancer Res 51: 3056-3058
21. Thiagalingam S, Lisitsyn NA, Hamaguchi M, Wigler MH, Willson JKV, Markowitz SD, Leach FS, Kinzler KW, Vogelstein B (1996) Evaluation of the FHIT gene in colorectal cancers. Cancer Res 56: 2936-2939
22. Vogelstein B, Fearon ER, Hamilton SR, Hamilton S, Kern SE, Preisinger AC, Leppert M, Nakamura Y, White R, Smits AMM, Bos JE (1988) Genetic alterations during colorectal-tumor development. N Eng J Med 319: 525-532
23. Vogelstein B, Fearon ER, Kern SE, Hamilton SR, Preisinger AC, Nakamura Y, White R (1989) Allelotype of colorectal carcinomas. Science 244: 207-211
24. Wagata T, Ishizaki K, Imamura M, Shimada Y, Ikenaga M, Tobe T (1991) Detection of 17p and amplification of the int-2 gene in esophageal carcinomas. Cancer Res 57: 2133-2117
25. Wright PA, Quirke P, Attanoos R, Williams GT (1992) Molecular pathology of gastric carcinoma: Progress and prospects. Human Pathol 23: 848-859
26. Zeng ZS, Hsu S, Zhang ZF, Cohen AM, Enker WE, Turnbull AA, Guillem JG (1994) High level of nm23 H-1 gene expression is associated with local colorectal cancer progression, not with metastasis. Br J Cancer 70: 1025-1030