Microbial community composition and function in wastewater treatment plants

Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology

Volumn 81, Issue 1-4, 2002, Pages 665-680

  Wagner, Michael ^a   Loy, Alexander ^a   Nogueira, Regina ^a   Purkhold, Ulrike ^a   Lee, Natuschka ^a   Daims, Holger ^a  

^a TECHNICAL UNIVERSITY OF MUNICH   (Germany)

Author keywords

FISH; Microautoradiography; Microbial ecology; Nitrogen removal; Phosphorous removal; Wastewater treatment

Indexed keywords

NITROGEN; PHOSPHORUS; RNA 16S;

COMMUNITY COMPOSITION; ECOSYSTEM FUNCTION; MICROBIAL COMMUNITY; MICROBIAL ECOLOGY; WASTEWATER;

ACCURACY; ACTINOBACTERIA; ACTIVATED SLUDGE; ARTICLE; BACTERIUM CULTURE; BACTERIUM IDENTIFICATION; BACTEROIDES; BIOFILM; CONTROLLED STUDY; ENVIRONMENTAL FACTOR; FLUORESCENCE IN SITU HYBRIDIZATION; FOAMING; GRAM NEGATIVE BACTERIUM; METHODOLOGY; MICROBIAL DIVERSITY; MICROFLORA; NITROBACTER; NITROSOMONAS; NONHUMAN; PRIORITY JOURNAL; UNINDEXED SEQUENCE; WASTE WATER MANAGEMENT;

BACTERIA; BIOFILMS; DNA, RIBOSOMAL; ECOSYSTEM; IN SITU HYBRIDIZATION, FLUORESCENCE; PHYLOGENY; RADIOGRAPHY; RNA, RIBOSOMAL, 16S; SEWAGE; WASTE DISPOSAL, FLUID; WATER MICROBIOLOGY;

BACTERIA (MICROORGANISMS); BACTEROIDES; NEGIBACTERIA; NITROBACTER; NITROSOMONAS;

EID: 0036418192     PISSN: 00036072     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1020586312170     Document Type: Article

References (93)

1. Amann R, Snaidr J, Wagner M, Ludwig W & Schleifer KH (1996) In situ visualization of high genetic diversity in a natural microbial community. J. Bacteriol. 178: 3496-3500.
2. Andreasen K & Nielsen PH (1997) Application of microautoradiography for the study of substrate uptake by filamentous microorganisms in activated sludge. Appl. Environ. Microbiol. 63: 3662-3668.
3. Attwood MA & Harder W (1972) A rapid and specific enrichment procedure for Hyphomicrobium spp. Antonie van Leeuwenhoek 38: 369-378.
4. Bayly RC, Duncan A, May JW, Pilkington NH, Raper WGC & Vasiliadis GE (1989). In: Pilkington NH & Bayly RC (Eds) AWWA 13th Federal Convention, (pp 162-166). Canberra.
5. Blackall LL, Stratton H, Bradford D, Dot TD, Sjorup C, Seviour EM & Seviour RJ (1996) Candidatus 'Microthrix parvicella', a filamentous bacterium from activated sludge sewage treatment plants. Int. J. Syst. Bacteriol. 46: 344-346.
6. Bond PL, Hugenholtz P, Keller J & Blackall LL (1995) Bacterial community structures of phosphate-removing and non-phosphate-removing activated sludges from sequencing batch reactors. Appl. Environ. Microbiol. 61: 1910-1916.
7. Bond PL, Erhart R, Wagner M, Keller J & Blackall LL (1999) Identification of some major groups of bacteria in efficient and nonefficient biological phosphorus removal activated sludge systems. Appl. Environ. Microbiol. 65: 4077-4084.
8. Bouchez T, Patureau D, Dabert P, Juretschko S, Doré J, Delgenès P, Moletta R & Wagner M (2000) Ecological study of a bioaugmentation failure. Environ. Microbiol. 2: 179-190.
9. Bradford D, Hugenholtz P, Seviour EM, Cunningham MA, Stratton H, Seviour RJ & Blackall LL (1996) 16S rRNA analysis of isolates obtained from gram-negative, filamentous bacteria micromanipulated from activated sludge. Syst. Appl. Microbiol. 19: 334-343.
10. Burrell PC, Keller J & Blackall LL (1998) Microbiology of a nitrite-oxidizing bioreactor. Appl. Environ. Microbiol. 64: 1878-1883.
11. Cech JS & Hartman P (1993) Competition between polyphosphate and polysaccharide accumulating bacteria in enhanced biological phosphate removal systems. Water Res. 27: 1219-1225.
12. Christensson M, Blackall LL & Welander T (1998) Metabolic transformations and characterisation of the sludge community in an enhanced biological phosphorus removal system. Appl. Microbiol. Biotechnol. 49: 226-234.
13. Cloete TE & Steyn PL (1987). In: Ramadori R (Ed) Biological Phosphate Removal from Wastewaters (pp 335-338). Pergamon Press, Oxford, UK.
14. Comeau Y, Hall KJ, Hancock REW & Oldham WK (1986) Biochemical model for enhanced biological phosphorus removal. Water Res. 20: 1511-1521.
15. Crocetti GR, Hugenholtz P, Bond PL, Schuler A, Keller J, Jenkins D & Blackall LL (2000) Identification of polyphosphate-accumulating organisms and design of 16S rRNA-directed probes for their detection and quantitation. Appl. Environ. Microbiol. 66: 1175-1182.
16. Crocetti GR, Banfield JF, Keller J, Bond PL & Blackall LL (2001) The identification of glycogen accumulating organisms from a poorly-operating enhanced biological phosphorus removal laboratory-scale SBR. 3rd IWA International Specialised Conference on Microorganisms in Activated Sludge and Biofilm Processes (poster 172) Rome, Italy.
17. Dabert P, Sialve B, Delgenes JP, Moletta R & Godon JJ (2001) Characterisation of the microbial 16S rDNA diversity of an aerobic phosphorus-removal ecosystem and monitoring of its transition to nitrate respiration. Appl. Microbiol. Biotech. 55: 500-509.
18. Daims H, Nielsen PH, Nielsen JL, Juretschko S & Wagner M (2000) Novel Nitrospira-like bacteria as dominant nitrite-oxidizers in biofilms from wastewater treatment plants: diversity and in situ physiology. Wat. Sci. Tech. 41: 85-90.
19. Daims H, Purkhold U, Bjerrum L, Arnold E, Wilderer PA & Wagner M (2001a) Nitrification in sequencing biofilm batch reactors: lessons from molecular approaches. Wat. Sci. Tech. 43: 9-18.
20. Daims H, Nielsen JL, Nielsen PH, Schleifer KH & Wagner M (2001b) In situ characterization of Nitrospira-like nitrite-oxidizing bacteria active in wastewater treatment plants. Appl. Environ. Microbiol. 67: 5273-5284.
21. de los Reyes MF, de los Reyes FL, 3rd, Hernandez M & Raskin L (1998) Quantification of Gordona amarae strains in foaming activated sludge and anaerobic digester systems with oligonucleotide hybridization probes. Appl. Environ. Microbiol. 64: 2503-2512.
22. Eikelboom DH (1975) Filamentous organisms observed in activated sludge. Water Res. 9: 365-388.
23. Erhart R, Bradford D, Seviour RJ, Amann R & Blackall LL (1997) Development and use of fluorescent in situ hybridization probes for the detection and identification of 'Microthrix parvicella' in activated sludge. Syst. Appl. Microbiol. 20: 310-318.
24. Fuhs GW & Chen M (1975) Microbiological basis of phosphate removal in the activated sludge process for the treatment of wastewater. Microbiol. Ecol. 2: 119-138.
25. Gieseke A, Purkhold U, Wagner M, Amann R & Schramm A (2001) Community structure and activity dynamics of nitrifying bacteria in a phosphate-removing biofilm. Appl. Environ. Microbiol. 67: 1351-1362.
26. Giovannoni SJ, Mullins TD & Field KG (1995). In: Joint J (Ed) Molecular Ecology of Aquatic Microbes. Springer-Verlag, Berlin, Heidelberg.
27. Guschin DY, Mobarry BK, Proudnikov D, Stahl DA, Rittmann BE & Mirzabekov AD (1997) Oligonucleotide microchips as genosensors for determinative and environmental studies in microbiology. Appl. Environ. Microbiol. 63: 2397-2402.
28. Henze M, Harremoës P, la Cour Jansen J & Arvin E (1997) Wastewater Treatment. Springer-Verlag, Berlin.
29. Hesselmann RPX, Werlen C, Hahn D, van der Meer JR & Zehnder AJB (1999) Enrichment, phylogenetic analysis and detection of a bacterium that performs enhanced biological phosphate removal in activated sludge. Syst. Appl. Microbiol. 22: 454-465.
30. Hiorns WD, Hastings RC, Head IM, McCarthy AJ, Saunders JR, Pickup RW & Hall GH (1995) Amplification of 16S ribosomal RNA genes of autotrophic ammonia-oxidizing bacteria demonstrates the ubiquity of nitrosospiras in the environment. Microbiology 141: 2793-2800.
31. Hiraishi A, Masamune K & Kitamura H (1989) Characterization of the bacterial population structure in an anaerobic-aerobic activated sludge system on the basis of respiratory quinone profiles. Appl. Environ. Microbiol. 55: 897-901.
32. Howarth R, Unz RF, Seviour EM, Seviour RJ, Blackall LL, Pickup RW, Jones JG, Yaguchi J & Head IM (1999) Phylogenetic relationships of filamentous sulfur bacteria (Thiothrix spp. and Eikelboom type 021N bacteria) isolated from wastewater-treatment plants and description of Thiothrix eikelboomii sp. nov., Thiothrix unzii sp. nov., Thiothrix fructosivorans sp. nov. and Thiothrix defluvii sp. nov. Int. J. Syst. Bacteriol. 49: 1817-1827.
33. Hugenholtz P, Goebel BM & Pace NR (1998) Impact of culture-independent studies on the emerging phylogenetic view of bacterial diversity. J. Bacteriol. 180: 4765-4774.
34. Jenkins D & Tandoi V (1991) The applied microbiology of enhanced biological phosphate removal - accomplishments and needs. Water Res. 25: 1471-1478.
35. Jenkins D, Richard MG & Daigger GT (1993) Manual on the Causes and Control of Activated Sludge Bulking and Foaming. Lewis Publishers, Boca Raton.
36. Juretschko S, Timmermann G, Schmid M, Schleifer KH, Pommerening-Roser A, Koops HP & Wagner M (1998) Combined molecular and conventional analyses of nitrifying bacterium diversity in activated sludge: Nitrosococcus mobilis and Nitrospira-like bacteria as dominant populations. Appl. Environ. Microbiol. 64: 3042-3051.
37. Juretschko S (2000) Mikrobielle Populationsstruktur und -dynamik in einer nitrifizierenden/ denitrifizierenden Belebtschlammanlage. Ph.D. Thesis, Technische Universität München.
38. Juretschko S, Loy A, Lehner A & Wagner M (2002) The microbial community composition of a nitrifying-denitrifying activated sludge from an industrial sewage treatment plant analyzed by the full-cycle rRNA approach. Syst. Appl. Microbiol. 25: 84-99.
39. Kämpfer P, Erhart R, Beimfohr C, Böhringer J, Wagner M & Amann R (1996) Characterization of bacterial communities from activated sludge: culture-dependent numerical identification versus in situ identification using group- and genus-specific rRNA-targeted oligonucleotide probes. Microb. Ecol. 32: 101-121.
40. Kanagawa T, Kamagata Y, Aruga S, Kohno T, Horn M & Wagner M (2000) Phylogenetic analysis of and oligonucleotide probe development for Eikelboom type 021N filamentous bacteria isolated from bulking activated sludge. Appl. Environ. Microbiol. 66: 5043-5052.
41. Kawaharasaki M, Kanagawa T, Tanaka H & Nakamura K (1998) Development and application of 16S rRNA-targeted oligonucleotide probe for detection of the phosphate-accumulating bacterium Microlunatus phosphovorus in an enhanced biological phosphorus removal process. Water Sci. Tech. 37: 481-484.
42. Knowles R (1982) Denitrification. Microbiol. Rev. 46: 43-70.
43. Lee N, Nielsen PH, Andreasen KH, Juretschko S, Nielsen JL, Schleifer K-H & Wagner M (1999) Combination of fluorescent in situ hybridization and microautoradiography - a new tool for structure-function analyses in microbial ecology. Appl. Environ. Microbiol. 65: 1289-1297.
44. Lee N, Jansen J, Aspegren H, Dircks K, Henze M, Schleifer K-H & Wagner M (2001) Population dynamics and in situ physiology of phosphorus-accumulating bacteria in wastewater treatment plants with enhanced biological phoshorus removal operated with and without nitrogen removal. 3rd IWA International Specialised Conference on Microorganisms in Activated Sludge and Biofilm Processes (pp 114-122). Rome, Italy.
45. Liu WT, Marsh TL, Cheng H & Forney LJ (1997) Characterization of microbial diversity by determining terminal restriction fragment length polymorphisms of genes encoding 16S rRNA. Appl. Environ. Microbiol. 63: 4516-4522.
46. Liu WT, Linning KD, Nakamura K, Mino T, Matsuo T & Forney LJ (2000) Microbial community changes in biological phosphate-removal systems on altering sludge phosphorus content. Microbiology 146: 1099-1107.
47. Liu WT, Nielsen AT, Wu JH, Tsai CS, Matsuo Y & Molin S (2001) In situ identification of polyphosphate- and polyhydroxyalkanoate-accumulating traits for microbial populations in a biological phosphorus removal process. Environ. Microbiol. 3: 110-122.
48. Lötter LH & Murphy M (1985) The identification of heterotrophic bacteria in an activated sludge plant with particular reference to polyphosphate accumulation. Water SA 11: 179-184.
49. Loy A, Daims H & Wagner M (2002). In: Bitton G (Ed) The Encyclopedia of Environmental Microbiology (pp 26-43). Wiley, New York.
50. Manz W, Wagner M, Amann R & Schleifer K-H (1994) In situ characterization of the microbial consortia active in two wastewater treatment plants. Water Res. 28: 1715-1723.
51. Manz W, Amann R, Ludwig W, Vancanneyt M & Schleifer K-H (1996) Application of a suite of 16S rRNA-specific oligonucleotide probes designed to investigate bacteria of the phylum cytophaga-flavobacter-bacteroides in the natural environment. Microbiology 142: 1097-1106.
52. Meyerhans A, Vartanian JP & Wain-Hobson S (1990) DNA recombination during PCR. Nucl. Acids Res. 18: 1687-1691.
53. Mino T, Arun V, Tsuzuki Y & Matsuo T (1987). In: Ramodori R (Ed) Biological Phosphate Removal from Wastewaters (pp 27-38). Pergamon Presss, Oxford.
54. Mino T, van Loosdrecht MCM & Heijnen JJ (1998) Microbiology and biochemistry of the enhanced biological phosphate removal process. Water Res. 32: 3193-3207.
55. Mino T (2000) Microbial selection of polyphosphate-accumulating bacteria in activated sludge wastewater treatment processes for enhanced biological phosphate removal. Biochemistry (Mosc) 65: 341-348.
56. Nakamura K, Masuda K & Mikami E (1991) Isolation of a new type of polyphosphate accumulating bacterium and its phosphate removal characteristics. J. Ferment. Bioeng. 71: 258-263.
57. Nakamura K, Hiraishi A, Yoshimi Y, Kawaharasaki M, Masuda K & Kamagata Y (1995) Microlunatus phosphovorus gen. nov., sp. nov., a new gram-positive polyphosphate-accumulating bacterium isolated from activated sludge. Int. J. Syst. Bacteriol. 45: 17-22.
58. Neef A, Zaglauer A, Meier H, Amann R, Lemmer H & Schleifer KH (1996) Population analysis in a denitrifying sand filter: conventional and in situ identification of Paracoccus spp. in methanol-fed biofilms. Appl. Environ. Microbiol. 62: 4329-4339.
59. Nielsen AT, Liu WT, Filipe C, Grady L, Molin S & Stahl DA (1999) Identification of a novel group of bacteria from a deteriorated biological phosphorus removal reactor. Appl. Environ. Microbiol. 65: 1251-1258.
60. Nielsen PH, Andreasen K, Wagner M, Blackall LL, Lemmer H & Seviour RJ (1998) Variability of Type 021N inactivated sludge as determined by in situ substrate uptake pattern and in situ hybridization with fluorescent rRNA-targeted probes. Wat. Sci. Tech. 37: 423-430.
61. Nielsen PH, de Muro MA & Nielsen JL (2000) Studies on the in situ physiology of Thiothrix spp. present in activated sludge. Environ. Microbiol. 2: 389-398.
62. Nielsen PH, Roslev P, Dueholm TE & Lund Nielsen J (2001) Microthrix parvicella, a specialized lipid consumer in anaerobic-aerobic activated sludge plants. 3rd IWA International Specialised Conference on Microorganisms in Activated Sludge and Biofilm Processes (pp 35-43). Rome, Italy.
63. Nyberg U, Aspegren H, Andersson B, Jansen JC & Villadsen IS (1992) Full-scale application of nitrogen removal with methanol as carbon source. Water Sci. Technol. 26: 1077-1086.
64. Okabe S, Satoh H & Watanabe Y (1999) In situ analysis of nitrifying biofilms as determined by in situ hybridization and the use of microelectrodes. Appl. Environ. Microbiol. 65: 3182-3191.
65. Purkhold U, Pommering-Röser A, Juretschko S, Schmid MC, Koops H-P & Wagner M (2000) Phylogeny of all recognized species of ammonia oxidizers based on comparative 16S rRNA and amoA sequence analysis: implications for molecular diversity surveys. Appl. Environ. Microbiol. 66: 5368-5382.
66. Rittmann BE & Whiteman R (1994) Bioaugmentation: a coming of age. Biotechnology 1: 12-16.
67. Satoh H, Mino T & Matsuo T (1992) Uptake of organic substrates and accumulation of polyhydroxyalkanoates linked with glycolysis of intracellular carbohydrates under anaerobic conditions in the biological excess phosphate removal processes. Water Sci. Tech. 26: 933-942.
68. Schleper C, DeLong EF, Preston CM, Feldman RA, Wu K-Y & Swanson RV (1998) Genomic analysis reveals chromosomal variation in natural populations of the uncultured psychrophilic archaeon Cenarchaeum symbiosum. J. Bacteriol. 180: 5003-5009.
69. Schmid M, Twachtmann U, Klein M, Strous M, Juretschko S, Jetten M, Metzger JW, Schleifer KH & Wagner M (2000) Molecular evidence for genus level diversity of bacteria capable of catalyzing anaerobic ammonium oxidation. Syst. Appl. Microbiol. 23: 93-106.
70. Schmid M, Schmitz-Esser S, Jetten M & Wagner M (2001) 16S-23S rDNA intergenic spacer and 23S rDNA of anaerobic ammonium-oxidizing bacteria: implications for phylogeny and in situ detection. Environ. Microbiol. 3: 450-459.
71. Schmider F & Ottow JCG (1986) Charakterisierung der denitrifizierenden Mikroflora in den verschiedenen Reinigungsstufen einer biologischen Kläranlage. Arch. Hydrobiol. 106: 497-516.
72. Schramm A, Larsen LH, Revsbech NP, Ramsing NB, Amann R & Schleifer K-H (1996) Structure and function of a nitrifying biofilm as determined by in situ hybridization and the use of microelectrodes. Appl. Environ. Microbiol. 62: 4641-4647.
73. Schramm A, De Beer D, Wagner M & Amann R (1998) Identification and activities in situ of Nitrosospira and Nitrospira spp. as dominant populations in a nitrifying fluidized bed reactor. Appl. Environ. Microbiol. 64: 3480-3485.
74. Schramm A, de Beer D, van den Heuvel JC, Ottengraf S & Amann R (1999) Microscale distribution of populations and activities of Nitrosospira and Nitrospira spp. along a macroscale gradient in a nitrifying bioreactor: quantification by in situ hybridization and the use of microsensors. Appl. Environ. Microbiol. 65: 3690-3696.
75. Schramm A, De Beer D, Gieseke A & Amann R (2000) Microenvironments and distribution of nitrifying bacteria in a membrane-bound biofilm. Environ. Microbiol. 2: 680-686.
76. Seviour EM, Blackall LL, Christensson C, Hugenholtz P, Cunningham MA, Bradford D, Stratton HM & Seviour RJ (1997) The filamentous morphotype Eikelboom Type 1863 is not a single genetic entity. J. Appl. Microbiol. 82: 411-421.
77. Seviour RJ, Maszenan AM, Soddell JA, Tandoi V, Patel BK, Kong Y & Schumann P (2000) Microbiology of the 'G-bacteria' in activated sludge. Environ. Microbiol. 2: 581-593.
78. Singleton DR, Furlong MA, Rathbun SL & Whitman WB (2001) Quantitative comparisons of 16S rRNA gene sequence libraries from environmental samples. Appl. Environ. Microbiol. 67: 4374-4376.
79. Snaidr J, Amann R, Huber I, Ludwig W & Schleifer K-H (1997) Phylogenetic analysis and in situ identification of bacteria in activated sludge. Appl. Environ. Microbiol. 63: 2884-2896.
80. Snaidr J, Beimfohr C, Levantesi C, Rossetti S, van der Waarde J, Geurkink B, Eikelboom D, Lemaitre M & Tandoi V (2001) Phylogenetic analysis and in situ identification of Nostocoida limicola-like filamentous bacteria in activated sludge from industrial wastewater treatment plants. 3rd IWA International Specialised Conference on Microorganisms in Activated Sludge and Biofilm Processes (pp 61-67). Rome, Italy.
81. Sperl GT & Hoare DS (1971) Denitrification with methanol: a selective enrichment for Hyphomicrobium species. J. Bacteriol. 108: 733-736.
82. Strous M, Fuerst JA, Kramer EHM, Logemann S, Muyzer G, van de Pas-Schoonen KT, Webb RI, Kuenen JG & Jetten MSM (1999) Missing lithotroph identified as new planctomycete. Nature 400: 446-449.
83. Strous M, Kuenen G, Fuerst JA, Wagner M & Jetten MSM (2002) The anammox case - a new experimental manifesto for microbiological eco-physiology. Antonie van Leeuwenhoek; this issue.
84. Suzuki M, Rappe MS & Giovannoni SJ (1998) Kinetic bias in estimates of coastal picoplankton community structure obtained by measurements of small-subunit rRNA gene PCR amplicon length heterogeneity. Appl. Environ. Microbiol. 64: 4522-4529.
85. Suzuki MT & Giovannoni SJ (1996) Bias caused by template annealing in the amplification of mixtures of 16S rRNA genes by PCR. Appl. Environ. Microbiol. 62: 625-630.
86. Timmermans P & van Haute A (1983) Denitrification with methanol: fundamental study of the growth and denitrification capacity of Hyphomicrobium sp. Water Res. 17: 1249-1255.
87. van Loosdrecht MC, Smolders GJ, Kuba T & Heijnen JJ (1997) Metabolism of micro-organisms responsible for enhanced biological phosphorus removal from wastewater. Use of dynamic enrichment cultures. Antonie Van Leeuwenhoek 71: 109-116.
88. Vedenina IY & Govorukhina NI (1988) Formation of a methylotrophic denitifying coenosis in a sewage purification system for removal of nitrates. Mikrobiologiya 57: 320-328.
89. Wagner M, Amann R, Lemmer H & Schleifer K-H (1993) Probing activated sludge with oligonucleotides specific for proteobacteria: inadequacy of culture-dependent methods for describing microbial community structure. Appl. Environ. Microbiol. 59: 1520-1525.
90. Wagner M, Amann R, Kämpfer P, Assmus B, Hartmann A, Hutzler P, Springer N & Schleifer K-H (1994) Identification and in situ detection of gram-negative filamentous bacteria in activated sludge. Syst. Appl. Microbiol. 17: 405-417.
91. Wagner M, Aßmuss B, Hartmann A, Hutzler P & Amann R (1994) In situ analysis of microbial consortia in activated sludge using fluorescently labelled, rRNA-targeted oligonucleotide probes and confocal laser scanning microscopy. J. Microsc. 176: 181-187.
92. Wagner M, Erhart R, Manz W, Amann R, Lemmer H, Wedi D & Schleifer K-H (1994) Development of an rRNA-targeted oligonucleotide probe specific for the genus Acinetobacter and its application for in situ monitoring in activated sludge. Appl. Environ. Microbiol. 60: 792-800.
93. Wagner M, Rath G, Koops H-P, Flood J & Amann R (1996) In situ analysis of nitrifying bacteria in sewage treatment plants. Wat. Sci. Tech. 34: 237-244.