The C-terminal extension of the small GTPase Ran is essential for defining the GDP-bound form

Journal of Molecular Biology

Volumn 318, Issue 2, 2002, Pages 583-593

  Nilsson, Jakob ^a   Weis, Karsten ^b   Kjems, Jørgen ^a  

^a AARHUS UNIVERSITY   (Denmark)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

GTPase; Importin family; NTF2; Nuclear import; Ran

Indexed keywords

BINDING PROTEIN; CARRIER PROTEIN; DIGITONIN; EXPORTIN T; GUANOSINE DIPHOSPHATE; GUANOSINE TRIPHOSPHATASE; IMPORTIN 7; KARYOPHERIN BETA; MUTANT PROTEIN; PROTEIN NTF2; PROTEIN SUBUNIT; RAN PROTEIN; TRANSPORTIN; UNCLASSIFIED DRUG;

ARTICLE; BINDING AFFINITY; CARBOXY TERMINAL SEQUENCE; CELL MEMBRANE PERMEABILITY; CONTROLLED STUDY; DISSOCIATION; EGG; GENE MUTATION; HUMAN; HUMAN CELL; MICROTUBULE; MITOSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN CONFORMATION; XENOPUS;

ASTER;

EID: 0036308740     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-2836(02)00040-2     Document Type: Article

References (46)

1. RanGAP1 induces GTPase activity of nuclear Ras-related Ran
2. Human RanGTPase-activating protein RanGAP1 is a homologue of yeast Rna1p involved in mRNA processing and transport
3. Mitotic regulator protein RCC1 is complexed with a nuclear ras-related polypeptide
4. Catalysis of guanine nucleotide exchange on Ran by the mitotic regulator RCC1
5. A small ubiquitin-related poly-peptide involved in targeting RanGAP1 to nuclear pore complex protein RanBP2
6. A novel ubiquitin-like modification modulates the partitioning of the Ran-GTPase-activating protein RanGAP1 between the cytosol and the nuclear pore complex
7. Chromatin docking and exchange activity enhancement of RCC1 by histones H2A and H2B
8. Identification of different roles for RanGDP and RanGTP in nuclear protein import
9. The asymmetric distribution of the constituents of the Ran system is essential for transport into and out of the nucleus
10. Characterization of the nuclear protein import mechanism using Ran mutants with altered nucleotide binding specificities
11. Generation of GTP-bound Ran by RCC1 is required for chromatin-induced mitotic spindle formation
12. The ran GTPase regulates mitotic spindle assembly
13. Stimulation of microtubule aster formation and spindle assembly by the small GTPase Ran
14. Self-organization of microtubule asters induced in Xenopus egg extracts by GTP-bound Ran
15. Ran-GTP stabilises microtubule asters and inhibits nuclear assembly in Xenopus egg extracts
16. Transport between the cell nucleus and the cytoplasm
17. Importins and exportins: How to get in and out of the nucleus
18. A role for RanBP1 in the release of CRM1 from the nuclear pore complex in a terminal step of nuclear export
19. RanGTP-regulated interactions of CRM1 with nucleoporins and a shuttling DEAD-box helicase
20. RanBP1 is crucial for the release of RanGTP from importin beta-related nuclear transport factors
21. NTF2 mediates nuclear import of Ran
22. Nuclear import of Ran is mediated by the transport factor NTF2
23. Ran induces spindle assembly by reversing the inhibitory effect of importin alpha on TPX2 activity
24. Importin beta is a mitotic target of the small GTPase Ran in spindle assembly
25. Role of importin-beta in coupling Ran to downstream targets in microtubule assembly
26. Structure of the nuclear transport complex karyopherin-beta2-Ran x GppNHp
27. Structure of a Ran-binding domain complexed with Ran bound to a GTP analogue: Implications for nuclear transport
28. Structural view of the Ran-Importin beta interaction at 2.3 Å resolution
29. Crystal structure of the nuclear Ras-related protein Ran in its GDP-bound form
30. Structural basis for molecular recognition between nuclear transport factor 2 (NTF2) and the GDP-bound form of the Ras-family GTPase Ran
31. Separate domains of the Ran GTPase interact with different factors to regulate nuclear protein import and RNA processing
32. The C terminus of the nuclear RAN/TC4 GTPase stabilizes the GDP-bound state and mediates interactions with RCC1, RAN-GAP, and HTF9A/RANBP1
33. A novel class of RanGTP binding proteins
34. The conserved amino-terminal domain of hSRP1 alpha is essential for nuclear protein import
35. Ran-unassisted nuclear migration of a 97-kD component of nuclear pore-targeting complex
36. Different binding domains for Ran-GTP and Ran-GDP/RanBP1 on nuclear import factor p97
37. Dominant-negative mutants of importin-beta block multiple pathways of import and export through the nuclear pore complex
38. The human homologue of yeast CRM1 is in a dynamic sub-complex with CAN/Nup214 and a novel nuclear pore component Nup88
39. Identification of a tRNA-specific nuclear export receptor
40. Dissecting the interactions between NTF2, RanGDP, and the nucleoporin XFXFG repeats
41. Mutations within the Ran/TC4 GTPase. Effects on regulatory factor interactions and subcellular localization
42. A role for the basic patch and the C terminus of RanGTP in regulating the dynamic interactions with importin beta, CRM1 and RanBP1
43. Selective disruption of nuclear import by a functional mutant nuclear transport carrier
44. Isolation of a protein that is essential for the first step of nuclear protein import
45. RanBP1 stabilizes the interaction of Ran with p97 nuclear protein import
46. The direction of transport through the nuclear pore can be inverted