Oligomeric state of membrane transport proteins analyzed with blue native electrophoresis and analytical ultracentrifugation

Journal of Molecular Biology

Volumn 317, Issue 4, 2002, Pages 591-600

  Heuberger, Esther H M L ^a   Veenhoff, Liesbeth M ^a   Duurkens, Ria H ^a   Friesen, Robert H E ^b   Poolman, Bert ^a  

^a UNIVERSITY OF GRONINGEN   (Netherlands)

^b UNIVERSITY OF GRONINGEN   (Netherlands)

Author keywords

Analytical ultracentrifugation; Blue native electrophoresis; Freeze fracture electron microscopy; Membrane transport proteins; Quaternary structure

Indexed keywords

ANAZOLENE SODIUM; CARRIER PROTEIN; LACTOSE TRANSPORTER; PHOSPHOTRANSFERASE; UNCLASSIFIED DRUG; XYLOSIDE TRANSPORTER;

ANALYTICAL ERROR; ARTICLE; CONTROLLED STUDY; ELECTRON MICROSCOPY; ELECTROPHORESIS; LACTOBACILLUS; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN FAMILY; PROTEIN QUATERNARY STRUCTURE; ULTRACENTRIFUGATION;

LACTOBACILLUS; LACTOBACILLUS PENTOSUS;

EID: 0036299012     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2002.5416     Document Type: Article

References (40)

1. The quarternary molecular architecture of TetA, a secondary tetracycline transporter from Escherichia coli
2. Three-dimensional structure of the ion-coupled transport protein NhaA
3. Projection structure and molecular architecture of OxlT, a bacterial membrane transporter
4. Properties of permease dimer, a fusion protein containing two lactose permease molecules from Escherichia coli
5. Structural analysis of cloned plasma membrane proteins by freeze-fracture electron microscopy
6. UhpT, the sugar phosphate antiporter of Escherichia coli, functions as a monomer
7. Glucose transporter oligomeric structure determines transporter function. Reversible redox-dependent interconversions of tetrameric and dimeric GLUT1
8. + antiporter of Escherichia coli in the membrane and its functional and structural consequences
9. Analysis of the oligomeric state of band 3, the anion transport protein of the human erythrocyte membrane, by size exclusion high performance liquid chromatography. Oligomeric stability and origin of heterogeneity
10. The projection structure of EmrE, a proton-linked multidrug transporter from Escherichia coli, at 7 Å resolution
11. Negative dominance studies demonstrate the oligomeric structure of EmrE, a multidrug antiporter from Escherichia coli
12. Dominant negative isoform of rat norepinephrine transporter produced by alternative RNA splicing
13. Hydrodynamic properties and immunological identification of the sodium- and chloride-coupled glycine transporter
14. Oligomerization of the human serotonin transporter and of the rat GABA transporter 1 visualized by fluorescence resonance energy transfer microscopy in living cells
15. The formation of a disulfide cross-link between the two subunits demonstrates the dimeric structure of the mitochondrial oxoglutarate carrier
16. The phosphate carrier from yeast mitochondria. Dimerization is a prerequisite for function
17. Quaternary structure of the lactose transport protein of Streptococcus thermophilus in the detergent-solubilized and membrane-reconstituted state
18. Rotational mobility and orientational stability of a transport protein in lipid membranes
19. The lactose transport protein is a cooperative dimer with two sugar translocation pathways
20. Mannitol-specific enzyme II of the phosphoenolpyruvate-dependent phosphotransferase system of Escherichia coli: Physical size of enzyme IImtl and its domains IIBA and IIC in the active state
21. Dimeric enzyme IImtl of the E. coli phosphoenolpyruvate-dependent phosphotransferase system. Cross-linking studies with bifunctional sulfhydryl reagents
22. Subunit and amino acid interactions in the Escherichia coli mannitol permease: A functional complementation study of coexpressed mutant permease proteins
23. Relation between the oligomerization state and the transport and phosphorylation function of the Escherichia coli mannitol transport protein: Interaction between mannitol-specific enzyme II monomers studied by complementation of inactive site-directed mutants
24. The 5 Å projection structure of the transmembrane domain of the mannitol transporter enzyme II
25. Reconstitution of Sec-dependent membrane protein insertion: Nascent FtsQ interacts with YidC in a SecYEG-dependent manner
26. Interaction between proteins localized in membranes
27. Molecular determinants of glycine receptor subunit assembly
28. Surface-localized glycine transporters 1 and 2 function as monomeric proteins in Xenopus oocytes
29. P2X1 and P2X3 receptors form stable trimers: A novel structural motif of ligand-gated ion channels
30. Blue native PAGE as a useful method for the analysis of the assembly of distinct combinations of nicotinic acetylcholine receptor subunits
31. Blue native electrophoresis for isolation of membrane protein complexes in enzymatically active form
32. Analysis of molecular masses and oligomeric states of protein complexes by blue native electrophoresis and isolation of membrane protein complexes by two-dimensional native electrophoresis
33. +)-dependent citrate carrier of Klebsiella pneumoniae
34. Xyloside transport by XylP, a member of the galactoside-pentoside-hexuronide family
35. Restrictive use of detergents in the functional reconstitution of the secondary multidrug transporter LmrP
36. The purified and functionally reconstituted multidrug transporter LmrA of Lactococcus lactis mediates the transbilayer movement of specific fluorescent phospholipids
37. Unidirectional reconstitution into detergent-destabilized liposomes of the purified lactose transport system of Streptococcus thermophilus
38. Sensitive monitoring of the dynamics of a membrane-bound transport protein by tryptophan phosphorescence spectroscopy
39. Molecular characterization of proteins in detergent solutions