Reconstitution of Sec-dependent membrane protein insertion: Nascent FtsQ interacts with YidC in a Sec YEG-dependent manner

EMBO Reports

Volumn 2, Issue 6, 2001, Pages 519-523

  Van Der Laan, Martin ^a   Houben, Edith N G ^b   Nouwen, Nico ^a   Luirink, Joen ^b   Driessen, Arnold J M ^a  

^a UNIVERSITY OF GRONINGEN   (Netherlands)

^b VU UNIVERSITY AMSTERDAM   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; CARRIER PROTEIN; MEMBRANE PROTEIN; PROTEIN PRECURSOR; PROTEIN SECA; SECRETORY PROTEIN; SECY PROTEIN; UNCLASSIFIED DRUG; YIDC PROTEIN;

ARTICLE; COMPLEX FORMATION; CONTROLLED STUDY; ESCHERICHIA COLI; GENE INSERTION; HOMOGENATE; MEMBRANE RECONSTITUTION; MOLECULAR INTERACTION; NONHUMAN; PRIORITY JOURNAL; PROTEIN CROSS LINKING; PROTEIN PURIFICATION; PROTEIN TRANSPORT; STAGING;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0034959718     PISSN: 1469221X     EISSN: None     Source Type: Journal    
DOI: 10.1093/embo-reports/kve106     Document Type: Article

References (28)

1. Sec-dependent and Sec-independent assembly of E.coli inner membrane proteins: The topological rules depend on chain length
2. In vivo degradation of secreted fusion proteins by the Escherichia coli outer membrane protease OmpT
3. Discrimination between SRP- and SecA/SecB-dependent substrates involves selective recognition of nascent chains by SRP and trigger factor
4. The E. coli SRP: Preferences of a targeting factor
5. Biosynthetic method for introducing unnatural amino acids site-specifically into proteins
6. Membrane translocation of mitochondrially coded Cox2p: Distinct requirements for export of N- and C-termini and dependence on the conserved protein Oxa1p
7. Oxa1p mediates the export of the N- and C-termini of pCoxII from the mitochondrial matrix to the intermembrane space
8. Oxa1p, an essential component of the N-tail protein export machinery in mitochondria
9. Requirements for the membrane insertion of signal-anchor type proteins
10. Nascent Lep inserts into the Escherichia coli inner membrane in the vicinity of YidC, SecY and SecA
11. Cysteine-directed cross-linking demonstrates that helix 3 of SecE is close to helix 2 of SecY and helix 3 of a neighboring SecE
12. Distinct domains of an oligotopic membrane protein are Sec-dependent and Sec-independent for membrane insertion
13. SecA protein hydrolyzes ATP and is an essential component of the protein translocation ATPase of Escherichia coli
14. An alternative protein targeting pathway in Escherichia coli: Studies on the role of FtsY
15. Escherichia coli translocase: The unravelling of a molecular machine
16. SecYEG assembles into a tetramer to form the active protein translocase channel
17. SRP-dependent co-translational targeting and SecA-dependent translocation analyzed as individual steps in the export of a bacterial protein
18. Evolutionarily conserved binding of ribosomes to the translocation channel via the large ribosomal RNA
19. YidC mediates membrane protein insertion in bacteria
20. Blue native electrophoresis for isolation of membrane protein complexes in enzymatically active form
21. SecA is not required for signal recognition particle-mediated targeting and initial membrane insertion of a nascent inner membrane protein
22. YidC, the Escherichia coli homologue of mitochondrial Oxa1p, is a component of the Sec translocase
23. Sec-dependent membrane protein insertion: Sequential interaction of nascent FtsQ with SecY and YidC
24. Early events in preprotein recognition in E. coli: Interaction of SRP and trigger factor with nascent polypeptides
25. Nascent membrane and presecretory proteins synthesized in Escherichia coli associate with signal recognition particle and trigger factor
26. The Escherichia coli SRP and SecB targeting pathways converge at the translocon
27. Interaction between SecA and SecYEG in micellar solution and formation of the membrane-inserted state
28. Non-bilayer lipids stimulate the activity of the reconstituted bacterial protein translocase