Blue native page as a useful method for the analysis of the assembly of distinct combinations of nicotinic acetylcholine receptor subunits

Journal of Receptor and Signal Transduction Research

Volumn 19, Issue 1-4, 1999, Pages 493-507

  Nicke, A ^a   Rettinger, J ^a   Mutschier, E ^a   Schmalzing, G ^a  

^a GOETHE UNIVERSITY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ION CHANNEL; NICOTINIC RECEPTOR;

ANIMAL CELL; CONFERENCE PAPER; ION CURRENT; NONHUMAN; OOCYTE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ANALYSIS; PROTEIN ASSEMBLY; PROTEIN PURIFICATION; PROTEIN STRUCTURE; XENOPUS;

ANIMALIA;

EID: 0033041549     PISSN: 10799893     EISSN: None     Source Type: Journal    
DOI: 10.3109/10799899909036667     Document Type: Conference Paper

References (21)

1. Betz, H. Ligand-gated ion channels in the brain: the amino acid receptor superfamily. Neuron 5, 383-392, 1990.
2. Hollmann, M. and Heinemann, S. Cloned glutamate receptors. Annu. Rev. Neurosci. 17, 31-108, 1994.
3. North, R. A. P2X receptors: a third major class of ligand-gated ion channels. Ciba Found. Symp. 198, 91-105, 1996.
4. Langosch, D.; Thomas, L. and Betz, H. Conserved quarternary structure of ligand-gated ion channels: the postsynaptic glycine receptor is a pentamer. Proc. Natl. Acad. Sci. USA 85, 7394-7398, 1988.
5. Nayeem, N.; Green, T.P.; Martin, I.L. and Barnard, E.A. Quaternary structure of the native GABAA receptor determined by electron microscopic image analysis. J. Neurochem. 62, 815-818, 1994.
6. 3 receptor. J. Neurochem. 64, 1401-1405, 1995.
7. Orteils, M.O. and Lunt, G.G. Evolutionary history of the ligand-gated ion-channel superfamily of receptors. Trends. Neurosci. 18, 121-127, 1995.
8. Schägger, H. and von Jagow, G. Blue native electrophoresis for isolation of membrane protein complexes in enzymatically active form. Anal. Biochem. 199, 223-231, 1991.
9. Schägger, H.; Cramer, W.A. and von Jagow, G. Analysis of molecular masses and oligomeric states of protein complexes by blue native electrophoresis and isolation of membrane protein complexes by two-dimensional native electrophoresis. Anal. Biochem. 217, 220-230, 1994.
10. 3 receptors form stable trimers: a novel structural motif of ligand-gated ion channels. EMBO J. 17, 3016-3028, 1998.
11. Green, W.N. and Millar, N.S. Ion-channel assembly. Trends. Neurosci. 18, 280-287, 1995.
12. Green, W.N. and Claudio, T. Acetylcholine receptor assembly: subunit folding and oligomerization occur sequentially. Cell 74, 57-69, 1993.
13. Witzemann, V.; Stein, E.; Barg, B.; Konno, T.; Koenen, M.; Kues, W.; Criado, M.; Hofmann, M. and Sakmann, B. Primary structure and functional expression of the alpha-, beta-, gamma- , delta- and epsilon-subunits of the acetylcholine receptor from rat muscle. Eur. J Biochem. 194, 437-448, 1990.
14. Schmalzing, G.; Kröner, S.; Schachner, M. and Gloor, S. The adhesion molecule on glia (AMOG/ßB2) and α1 subunits assemble to functional sodium pumps in Xenopus oocytes. J. Biol. Chem. 267, 20212-20216, 1992.
15. Thompson, J.A.; Lau, A.L. and Cunningham, D.D. Selective radiolabeling of cell surface proteins to a high specific activity. Biochemistry USA 26, 743-750, 1987.
16. Unwin, N. Acetylcholine receptor channel imaged in the open state. Nature 373, 37-43, 1995.
17. Liu, Y. and Brehm, P. Expression of subunit-omitted mouse nicotinic acetylcholine receptors in Xenopus laevis oocytes. J. Physiol. Lond. 470, 349-363, 1993.
18. Kurosaki, T.; Fukuda, K.; Konno, T.; Mori, Y.; Tanaka, K.; Mishina, M. and Numa, S. Functional properties of nicotinic acetylcholine receptor subunits expressed in various combinations. FEBS Lett. 214, 253-258, 1987.
19. Saedi, M.S.; Conroy, W.G. and Lindstrom, J. Assembly of Torpedo acetylcholine receptors in Xenopus oocytes. J. Cell Biol. 112, 1007-1015, 1991.
20. Blount, P. and Merlie, J.P. Molecular basis of the two nonequivalent ligand binding sites of the muscle nicotinic acetylcholine receptor. Neuron 3, 349-357, 1989.
21. Gu, Y.; Forsayeth, J.R.; Verrall, S.; Yu, X.M. and Hall, Z.W. Assembly of the mammalian muscle acetylcholine receptor in transfected COS cells. J Cell Biol. 114, 799-807, 1991.