The lactose transport protein is a cooperative dimer with two sugar translocation pathways

EMBO Journal

Volumn 20, Issue 12, 2001, Pages 3056-3062

  Veenhoff, L M ^a   Heuberger, E H M L ^a   Poolman, B ^a  

^a UNIVERSITY OF GRONINGEN   (Netherlands)

Author keywords

Cooperative; Dimer; Membrane protein; Quaternary structure; Sugar transport

Indexed keywords

CARRIER PROTEIN; CYSTEINE; DIMER; LACTOSE TRANSPORT PROTEIN; N ETHYLMALEIMIDE; SUGAR; UNCLASSIFIED DRUG;

ARTICLE; DIMERIZATION; ENZYME ACTIVITY; ENZYME CONFORMATION; PRIORITY JOURNAL; PROTEIN TRANSPORT; PROTEOLIPOSOME; PROTON MOTIVE FORCE;

CARBOHYDRATE METABOLISM; DIMERIZATION; ESCHERICHIA COLI PROTEINS; GALACTOSIDES; MEMBRANE TRANSPORT PROTEINS; MONOSACCHARIDE TRANSPORT PROTEINS; MUTAGENESIS, SITE-DIRECTED; OLIGOPEPTIDES; SYMPORTERS;

EID: 0035875919     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/20.12.3056     Document Type: Article

References (36)

1. UhpT, the sugar phosphate antiporter of Escherichia coli, functions as a monomer
2. Allosteric properties and the association equilibria of hemocyanin from Callianassa californiensis
3. Analysis of the oligomeric state of Band 3, the anion transport protein of the human erythrocyte membrane, by size exclusion high performance liquid chromatography. Oligomeric stability and origin of heterogeneity
4. Structural analysis of cloned plasma membrane proteins by freeze-fracture electron microscopy
5. + exchanger isoforms NHE1 and NHE3 exist as stable dimers in membranes with a high degree of specificity for homodimers
6. Quaternary structure of the lactose transport protein of Streptococcus thermophilus in the detergent-solubilized and membrane-reconstituted state
7. + antiporter of Escherichia coli in the membrane and its functional and structural consequences
8. Interaction between proteins localized in membranes
9. HPr(His-P)-mediated phosphorylation differently affects counterflow and proton motive force-driven uptake via the lactose transport protein of Streptococcus thermophilus
10. Phosphorylation state of HPr determines the level of expression and the extent of phosphorylation of the lactose transport protein of Streptococcus thermophilus
11. Phosphorylation and functional properties of the IIA domain of the lactose transport protein of Streptococcus thermophilus
12. Glucose transporter oligomeric structure determines transporter function. Reversible redox-dependent interconversions of tetrameric and dimeric GLUT1
13. A spectroscopic assay for the analysis of carbohydrate transport reactions
14. Unidirectional reconstitution into detergent-destabilized liposomes of the purified lactose transport system of Streptococcus thermophilus
15. Detergent-mediated reconstitution of membrane proteins
16. Uncoupling in secondary transport proteins. A mechanistic explanation for mutants of lac permease with an uncoupled phenotype
17. A consensus structure for membrane transport
18. 10-specified tetracycline efflux protein TetA contains a dimerization domain
19. − cotransporter (NKCCl) is a homodimer
20. Major facilitator superfamily
21. Microbial genome analyses: Global comparisons of transport capabilities based on phylogenies, bioenergetics and substrate specificities
22. Kinetic analysis of lactose and proton coupling in Glu379 mutants of the lactose transport protein of Streptococcus thermophilus
23. + symport by phosphoenolpyruvate-dependent enzyme I/HPr-mediated phosphorylation
24. Cation and sugar selectivity determinants in a novel family of transport proteins
25. Interdomain hybrid Tet proteins confer tetracycline resistance only when they are derived from closely related members of the tet gene family
26. Properties of permease dimer, a fusion protein containing two lactose permease molecules from Escherichia coli
27. Unraveling the mechanism of the lactose permease of Escherichia coli
28. Families of transmembrane sugar transport proteins
29. The phosphate carrier from yeast mitochondria. Dimerization is a prerequisite for function
30. Rotational mobility and orientational stability of a transport protein in lipid membranes
31. Substrate recognition at the cytoplasmic and extracellular binding site of the lactose transport protein of Streptococcus thermophilus
32. Close approximation of putative α-helices II, IV, VII, X and XI in the translocation pathway of the lactose transport protein of Streptococcus thermophilus
33. Three-dimensional map of the dimeric membrane domain of the human erythrocyte anion exchanger, Band 3
34. Three-dimensional structure of the ion-coupled transport protein NhaA
35. The quarternary molecular architecture of TetA, a secondary tetracycline transporter from Escherichia coli
36. Glucose transporter function is controlled by transporter oligomeric structure. A single, intramolecular disulfide promotes GLUT1 tetramerization