High level expression and purification of bioactive bovine interleukin-18 using a baculovirus system

Veterinary Immunology and Immunopathology

Volumn 87, Issue 1-2, 2002, Pages 65-72

  Nagata, Tomoshi ^a   Ishikawa, Satoko ^a   Shimokawa, Eiko ^a   Kamikawa, Makiyo ^a   Kikuma, Reiko ^a   Muneta, Yoshihiro ^b   Yokomizo, Yuichi ^b   Nakamura, Masayuki ^a   Takehara, Kazuaki ^a  

^a KITASATO UNIVERSITY   (Japan)

^b NATIONAL INSTITUTE OF ANIMAL HEALTH   (Japan)

Author keywords

Baculovirus; Bovine IL 12; Bovine IL 18; Expression; Purification; Synergistic effect

Indexed keywords

GAMMA INTERFERON; HISTIDINE; INTERLEUKIN 18; INTERLEUKIN 1BETA CONVERTING ENZYME; ISOPROTEIN; MONOCLONAL ANTIBODY; RECOMBINANT ENZYME; RECOMBINANT INTERLEUKIN 12; RECOMBINANT INTERLEUKIN 18;

ANIMAL CELL; ANIMAL MODEL; ARTICLE; BACULOVIRUS; CARBOXY TERMINAL SEQUENCE; CATTLE; CONTROLLED STUDY; CYTOKINE PRODUCTION; GENETIC RECOMBINATION; INSECT CELL; MONONUCLEAR CELL; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; SUPERINFECTION; SUPERNATANT; VIRUS RECOMBINANT;

ANIMALS; BACULOVIRIDAE; BLOTTING, WESTERN; CASPASE 1; CATTLE; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; INTERFERON TYPE II; INTERLEUKIN-18; RECOMBINANT PROTEINS; SPODOPTERA; SUPERINFECTION;

BOVINAE; INSECTA; SUIDAE; UNIDENTIFIED BACULOVIRUS;

EID: 0036281315     PISSN: 01652427     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-2427(02)00127-7     Document Type: Article

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