Comet assay as a novel approach for studying DNA damage in focal cerebral ischemia: Differential effects of NMDA receptor antagonists and poly(ADP-ribose) polymerase inhibitors

Journal of Cerebral Blood Flow and Metabolism

Volumn 22, Issue 6, 2002, Pages 697-704

  Giovannelli, Lisa ^a   Cozzi, Andrea ^a   Guarnieri, Ilaria ^a   Dolara, Piero ^a   Moroni, Flavio ^a  

^a UNIVERSITY OF FLORENCE   (Italy)

Author keywords

Double strand breaks; DPQ; MK 801; PARP; Rat brain; Single strand breaks

Indexed keywords

3,4 DIHYDRO 5 [4 (1 PIPERIDINYL)BUTOXYL] 1(2H) ISOQUINOLINE; DIZOCILPINE; DNA; N METHYL DEXTRO ASPARTIC ACID RECEPTOR BLOCKING AGENT; NICOTINAMIDE ADENINE DINUCLEOTIDE ADENOSINE DIPHOSPHATE RIBOSYLTRANSFERASE; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BRAIN CORTEX; BRAIN ISCHEMIA; CAUDATE NUCLEUS; COMET ASSAY; CONTROLLED STUDY; DNA STRAND BREAKAGE; HIPPOCAMPUS; MALE; MIDDLE CEREBRAL ARTERY OCCLUSION; NEUROMODULATION; NEUROPROTECTION; NONHUMAN; PATHOPHYSIOLOGY; PRIORITY JOURNAL; PUTAMEN; RAT;

EID: 0036263131     PISSN: 0271678X     EISSN: None     Source Type: Journal    
DOI: 10.1097/00004647-200206000-00008     Document Type: Article

References (50)

1. Hypersensitivity of human lymphocytes to UV-B and solar irradiation
2. Oxidative damage, bleomycin, and gamma radiation induce different types of DNA strand breaks in normal lymphocytes and thymocytes: A comet assay study
3. Elevation of extracellular concentrations of glutamate and aspartate in rat hippocampus during transient cerebral ischemia monitored by intracerebral microdialysis
4. Quantitative morphologic analysis of striatal cholinergic neurons in perinatal asphyxia
5. Ionic mechanisms underlying differential vulnerability to ischemia in striatal neurons
6. Early endonuclease activation following reversible focal ischemia in the rat brain
7. A study of the dose dependency of a glycine receptor antagonist in focal ischemia
8. Early detection of DNA strand breaks in the brain after transient focal ischemia: Implications for the role of DNA damage in apoptosis and neuronal cell death
9. Calcium-mediated neurotoxicity: Relationship to specific channel types and role in ischemic damage
10. Glutamate neurotoxicity and diseases of the nervous system
11. The kinetics of repair of oxidative DNA damage (strand breaks and oxidized pyrimidines) in human cells
12. Oxidative damage to DNA: Do we have a reliable biomarker?
13. Kynurenine hydroxylase inhibitors reduce ischemic brain damage: Studies with (m-nitrobenzoyl)-alanine (mNBA) and 3,4-dimethoxy-[-N-4-(nitrophenyl)thiazol-2yl]-benzenesulfonamide (Ro 61-8048) ill models of focal or global brain ischemia
14. Nitric oxide mediates glutamate neurotoxicity in primary cortical neurons
15. Poly (ADP-ribose) polymerase: A molecular nick-sensor
16. Poly(ADP-ribose)polymerase gene disruption renders mice resistant to cerebral ischemia
17. Ischemic brain injury is mediated by the activation of poly(ADP-ribose)polymerase
18. The comet assay: A comprehensive review
19. Selective sparing of NADPH diaphorase neurons in neonatal hypoxia-ischemia
20. Neuronal necrosis after middle cerebral artery occlusion in Wistar rats progresses at different time intervals in the caudoputamen and the cortex
21. Effect of complex polyphenols and tannins from red wine on DNA oxidative damage of rat colon mucosa in vivo
22. Effects of cerebral ischemia in mice deficient in neuronal nitric oxid synthase
23. Effects of postnatal hypoxia-ischemia on cholinergic neurons in the rat forebrain: Choline acetyltransferase immunocytochemistry
24. A subset of non-histone nuclear proteins reversibly stabilized by the sulfhydryl cross-linking reagent tetrathionate: Polypeptides of the internal nuclear matrix
25. The comet assay: Mechanisms and technical considerations
26. Caspase inhibitors reduce neuronal injury after focal but not global cerebral ischemia in rats
27. Damage, repair, and mutagenesis in nuclear genes after mouse forebrain ischemia-reperfusion
28. Inhibition of poly(ADP-ribose) polymerase: Reduction of ischemic injury and attenuation of N-methyl-D-aspartate-induced neurotransmitter dysregulation
29. GM1 ganglioside reduces ischemia-induced excitatory amino acid output: A microdialysis study in the gerbil hippocampus
30. Reversible middle cerebral artery occlusion without craniectomy in rats
31. Oxidative stress in brain ischemia
32. DNA damage in rat brain cells after in vivo exposure to 2450 MHz electromagnetic radiation and various methods of euthanasia
33. The single cell gel electrophoresis assay (comet assay): A European review
34. Possible therapeutic applications of antagonists of excitatory amino acid neurotransmitters
35. The comet assay as a rapid test in biomonitoring occupational exposure to DNA damaging agents and effect of confounding factors
36. Poly-(ADP-ribose) polymerase inhibitors attenuate necrotic but not apoptotic neuronal death in experimental models of cerebral ischemia
37. Induction of oxidative DNA damage in the peri-infarct region after permanent focal cerebral ischemia
38. Excitatory amino acid release and free radical formation may cooperate in the genesis of ischemia-induced neuronal damage
39. Detection of in vivo genotoxicity of 3-chloro-4-(dichloromethyl)-5-hydroxy-2[5H]-furanone (MX) by the alkaline single cell gel electrophoresis (Comet) assay in multiple mouse organs
40. Blockade of N-methyl-D-aspartate receptors may protect against ischemic damage in the brain
41. A simple technique for quantitation of low levels of DNA damage in individual cells
42. Continuing damage to rat retinal DNA during darkness following light exposure
43. Felbamate inhibits dihydropyridine-sensitive calcium channels in central neurons
44. DNA damage, cell kinetics and ODC activities studied in CBA mice exposed to electromagnetic fields generated by transmission lines
45. Triggering and execution of neuronal death in brain ischemia: Two phases of glutamate release by different mechanisms
46. The effects of reperfusion on neuroprotection using an inhibitor of poly(ADP-ribose) polymerase
47. Post-treatment with an inhibitor of poly (ADP-ribose) polymerase attenuates cerebral damage in focal ischemia
48. The alkaline single cell electrophoresis assay with eight mouse organs: Results with 22 monofunctional alkylating agents (including 9 dialkyl N-nitrosoamines) and 10 DNA crosslinkers
49. Bacteria used for the production of yogurt inactivate carcinogens and prevent DNA damage in the colon of rats
50. Nitric oxide activation of poly (ADP-ribose)synthetase in neurotoxicity