Accurate measurement of near-micromolar oxygen concentrations in aqueous solutions based on enzymatic extradiol cleavage of 4-chlorocatechol: Applications to improved low-oxygen experimental systems and quantitative assessment of back diffusion of oxygen from the atmosphere

Journal of Biochemistry

Volumn 131, Issue 4, 2002, Pages 523-531

  Nakajima, Hirofumi ^a   Ishida, Tetsuo ^a   Tanaka, Hiroyuki ^a   Horiike, Kihachiro ^a  

^a SHIGA UNIVERSITY OF MEDICAL SCIENCE   (Japan)

Author keywords

Anaerobic reaction; Catechol 2,3 dioxygenase; HPLC analysis; L ascorbate oxidase; Oxygen concentration

Indexed keywords

4 CHLOROCATECHOL; 5 CHLORO 2 HYDROXYMUCONATE SEMIALDEHYDE; ALDEHYDE; ANION; ARGON; ASCORBATE OXIDASE; CATECHOL; CATECHOL 2,3 DIOXYGENASE; CATECHOL DERIVATIVE; OXYGEN; REAGENT; UNCLASSIFIED DRUG;

ABSORPTION; ACCURACY; ANAEROBIC REACTOR; ANALYTIC METHOD; AQUEOUS SOLUTION; ARTICLE; ATMOSPHERE; CALIBRATION; CONTROLLED STUDY; ELECTRODE; ENZYME KINETICS; ENZYME MECHANISM; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MEASUREMENT; MICHAELIS CONSTANT; OXYGEN CONCENTRATION; OXYGEN DIFFUSION; PH; PSEUDOMONAS PUTIDA; QUANTITATIVE ANALYSIS; TEMPERATURE;

PSEUDOMONAS; PSEUDOMONAS PUTIDA;

EID: 0036242051     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a003130     Document Type: Article

References (31)

1. 2 and ATP
2. Defining the resistance to oxygen transfer in tissue hypoxia
3. Oxygen dependence of mitochondrial function in isolated rat cardiac myocytes
4. 2 dependence in kidney
5. Oxygen dependence of redox state of copper in cytochrome oxidase in vitro
6. m for oxygen: Implications for mitochondrial pathology
7. Sulfate reducers-dominant players in a low-oxygen world?
8. Formation of sphalerite (ZnS) deposits in natural biofilms of sulfate-reducing bacteria
9. Reactions of the Escherichia coli flavohaemoglobin (Hmp) with NADH and near-micromolar oxygen: Oxygen affinity of NADH oxidase activity
10. Identification of a cytochrome b-type NAD(P)H oxidoreductase ubiquitously expressed in human cells
11. + current modulation in wild-type and oxidase-deficient mice
12. How do cells sense oxygen?
13. 2 sensing
14. 2-regulated prolyl hydroxylation
15. 2, and the 3 PHDs: How animal cells signal hypoxia to the nucleus
16. C. elegans EGL-9 and mammalian homologs define a family of dioxygenases that regulate HIF by prolyl hydroxylation
17. Enzymatic method for measuring the absolute value of oxygen concentration
18. Calibration of a Clark-type oxygen electrode by tyrosinase-catalyzed oxidation of 4-tert-butylcatechol
19. Preparation of solutions of dissolved hydrogen and oxygen gases using an anaerobic chamber
20. Enzymatic method for measuring subnanomolar concentrations of oxygen in gaseous or aqueous solutions
21. Temperature titration: A new approach to the thermodynamics of oxygen binding to hemoglobin
22. Hemoglobin-oxygen equilibrium curves measured during enzymatic oxygen consumption
23. A perifusion system to control oxygen concentration in cell suspensions
24. A cell viability assay based on monitoring respiration by optical oxygen sensing
25. Rapid spectrophotometric determination of oxygen consumption using hemoglobin, in vitro: Light scatter correction and expanded dynamic range
26. The use of protocatechuate dioxygenase for maintaining anaerobic conditions in biochemical experiments
27. Dioxygen activation by enzyme with mononuclear non-heme iron active sites
28. Overexpression of Pseudomonas putida catechol 2,3-dioxygenase with high specific activity by genetically engineered Escherichia coli
29. An archetypical extradiol-cleaving catechol dioxygenase: The crystal structure of catechol 2,3-dioxygenase (metapyrocatechase) from Pseudomonas putida mt-2
30. A primitive pathway of porphyrin biosynthesis and enzymology in Desulfovibrio vulgaris
31. Calibration of oxygen-dependent quenching of the phosphorescence of pd-mesotetra (4-carboxyphenyl) porphine: A phosphor with general application for measuring oxygen concentration in biological systems