Binding site requirements of the virulence gene regulator AphB: Differential affinities for the Vibrio cholerae classical and El Tor tcpPH promoters

Molecular Microbiology

Volumn 44, Issue 2, 2002, Pages 533-547

  Kovacikova, Gabriela ^a   Skorupski, Karen ^a  

^a DARTMOUTH MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR APHB; TRANSCRIPTION FACTOR LYSR; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL GENE; BACTERIAL VIRULENCE; BASE PAIRING; BINDING AFFINITY; BINDING SITE; CONTROLLED STUDY; GENE CONTROL; GENE EXPRESSION REGULATION; GENE REPRESSION; MOLECULAR RECOGNITION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN BINDING; PROTEIN FUNCTION; PROTEIN MOTIF; SITE DIRECTED MUTAGENESIS; TRANSCRIPTION INITIATION; VIBRIO CHOLERAE;

BACTERIAL PROTEINS; BASE SEQUENCE; BINDING SITES; DNA FOOTPRINTING; DNA PRIMERS; FIMBRIAE PROTEINS; FIMBRIAE, BACTERIAL; GENOTYPE; HELIX-TURN-HELIX MOTIFS; KINETICS; PROMOTER REGIONS (GENETICS); TRANS-ACTIVATION (GENETICS); TRANS-ACTIVATORS; TRANSCRIPTION FACTORS; VIBRIO CHOLERAE; VIRULENCE;

ANIMALIA; BACTERIA (MICROORGANISMS); NEGIBACTERIA; TOR; VIBRIO; VIBRIO CHOLERAE; VIBRIO CHOLERAE O1 BIOVAR ELTOR;

EID: 0036229557     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.2002.02914.x     Document Type: Article

References (53)

1. H-NS: A modulator of environmentally regulated gene expression
2. Inducible metabolism of phenolic acids in Pediococcus pentosaceus is encoded by an autoregulated operon which involves a new class of negative transcriptional regulator
3. pepA, a gene mediating pH regulation of virulence genes in Vibrio cholerae
4. Organization of tcp, acf and toxT genes within a ToxT-dependent operon
5. The MetR binding site in the Salmonella typhimurium metH gene: DNA sequence constraints on activation
6. Phase variation in tcpH modulates expression of the ToxR regulon in Vibrio cholerae
7. A branch in the ToxR regulatory cascade of Vibrio cholerae revealed by characterization of toxT mutant strains
8. OxyR, a positive regulator of hydrogen-peroxide inducible genes in Escherichia coli and Salmonella typhimurium is homologous to a family of bacterial regulatory proteins
9. Bacterial virulence gene regulation: An evolutionary perspective
10. Analysis of ToxR-dependent transcription activation of ompU, the gene encoding a major envelope protein in Vibrio cholerae
11. Periplasmic interaction between two membrane regulatory proteins, ToxR and ToxS, results in signal transduction and transcriptional activation
12. Regulatory cascade controls virulence in Vibrio cholerae
13. The chromosomal virulence gene, chvE, of Agrobacterium tumefaciens is regulated by a LysR family member
14. Rapid production of full-length cDNAs from rare transcripts: Amplification using a single gene-specific oligonucleotide primer
15. Regulation of cholera toxin by temperature, pH and osmolarity
16. In vitro binding of the Salmonella dublin virulence plasmid regulatory protein SpvR to the promoter regions of spvA and SpvR
17. TcpP protein is a positive regulator of virulence gene expression in Vibrio cholerae
18. Toxin, toxin-coregulated pili, and the toxR regulon are essential for Vibrio cholerae colonization in humans
19. Transcriptional control of toxT, a regulatory gene in the ToxR regulon of Vibrio cholerae
20. The virulence gene activator ToxT from Vibrio cholerae is a member of the AraC family of transcriptional activators
21. Culture conditions for stimulating cholera toxin production by Vibrio cholerae O1 El Tor
22. Cholera
23. A Vibrio cholerae pathogenicity island associated with epidemic and pandemic strains
24. A bacteriophage encoding a pathogenicity island, a type-IV pilus and a phage receptor in cholera bacteria
25. A Vibrio cholerae LysR homolog, AphB, cooperates with AphA at the tcpPH promoter to activate expression of the ToxR virulence cascade
26. Differential activation of the tcpPH promoter by AphB determines biotype specificity of virulence gene expression in Vibrio cholerae
27. Overlapping binding sites for the virulence gene regulators AphA. AphB and cAMP-CRP at the Vibrio cholerae tcpPH promoter
28. The Vibrio choleraeToxR/TcpP/ToxT virulence cascade: Distinct roles for two membrane-localized transcriptional activators on a single promoter
29. Regulation and temporal expression patterns of Vibrio cholerae virulence genes during infection
30. Molecular cloning and transcriptional regulation of ompT, a ToxR-repressed gene in Vibrio cholerae
31. Structure-function studies on Escherichia coli MetR protein, a putative prokaryotic leucine zipper protein
32. A novel suicide vector and its use in construction of insertion mutations: Osmo-regulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR
33. Cholera toxin transcriptional activator ToxR is a transmembrane DNA binding protein
34. Identification of toxS, a regulatory gene whose product enhances ToxR-mediated activation of the cholera toxin promoter
35. Vibrio cholerae H-NS silences virulence gene expression at multiple steps in the ToxR regulatory cascade
36. Negative autoregulation of cysB in Salmonella typhimurium: In vitro interactions of CysB protein with the cysB promoter
37. Roles of CatR and cis, cis-muconate in activation of the catBC operon, which is involved in benzoate degradation in Pseudomonas putida
38. Positive control of transcription initiation in bacteria
39. Functional analysis of the Pseudomonas putida regulatory protein CatR: Transcriptional studies and determination of the CatR DNA-binding site by hydroxyl-radical footprinting
40. Molecular biology of the LysR family of transcriptional regulators
41. Environmental signals modulate ToxT-dependent virulence factor expression in Vibrio cholerae
42. In vivo analysis of the interactions of the LysR-like regulator SpvR with the operator sequences of the spvA and spvR virulence genes of Salmonella typhimurium
43. Improved single and multicopylac-based cloning vectors for protein and operon fusions
44. Positive selection vectors for allelic exchange
45. Control of the ToxR virulence regulon in Vibrio cholerae by environmental stimuli
46. Cyclic AMP and its receptor protein negatively regulate the coordinate expression of cholera toxin and toxin-coregulated pilus in Vibrio cholerae
47. A new level in the Vibrio cholerae ToxR virulence cascade: AphA is required for transcriptional activation of the tcpPH operon
48. Use of phoA gene fusions to identify a pilus colonization factor coordinately regulated with cholera toxin
49. Redox-dependent shift of OxyR-DNA contacts along an extended DNA-binding site: A mechanism for differential promoter selection
50. Genetic and biochemical analysis of the MetR activator-binding site in the metE metR control region of Salmonella typhimurium
51. Lysogenic conversion by a filamentous phage encoding cholera toxin
52. Analysis of an autore-gulatory loop controlling ToxT, cholera toxin, and toxin-coregulated pilus production in Vibrio cholerae