Differential activation of the tcpPH promoter by AphB determines biotpye specificity of virulence gene expression in Vibrio cholerae

Journal of Bacteriology

Volumn 182, Issue 11, 2000, Pages 3228-3238

  Kovacikova, Gabriela ^a   Skorupski, Karen ^a  

^a DARTMOUTH MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACTERIAL VIRULENCE; BASE PAIRING; BIOTYPOLOGY; GENE ACTIVATION; GENE EXPRESSION; GENE LOCATION; GENE SEQUENCE; GENETIC TRANSCRIPTION; NONHUMAN; PRIORITY JOURNAL; VIBRIO CHOLERAE;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BASE SEQUENCE; ESCHERICHIA COLI; FIMBRIAE PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; GENES, REPORTER; LAC OPERON; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; POINT MUTATION; PROMOTER REGIONS (GENETICS); RECOMBINANT FUSION PROTEINS; SPECIES SPECIFICITY; TRANS-ACTIVATORS; TRANSCRIPTION FACTORS; VIBRIO CHOLERAE;

EID: 0034072989     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.182.11.3228-3238.2000     Document Type: Article

References (38)

1. Brown, R. C., and R. K. Taylor. 1995. Organization of tcp, acf, and toxT genes within a ToxT-dependent operon. Mol. Microbiol. 16:425-439.
2. Byerly, K. A., M. L. Urbanowski, and G. V. Stauffer. 1991. The MetR binding site in the Salmonella typhimurium metH gene: DNA sequence constraints on activation. J. Bacteriol. 173:3547-3553.
3. Carroll, P. A., K. T. Tashima, M. B. Rogers, V. J. DiRita, and S. B. Calderwood. 1997. Phase variation in tcpH modulates expression of the ToxR regulon in Vibrio cholerae. Mol. Microbiol. 25:1099-1111.
4. Champion, G. A., M. N. Neely, M. A. Brennan, and V. J. DiRita. 1997. A branch in the ToxR regulatory cascade of Vibrio cholerae revealed by characterization of toxT mutant strains. Mol. Microbiol. 23:323-331.
5. Chang, M., and I. P. Crawford. 1991. In vitro determination of the effect of indoleglycerol phosphate on the interaction of purified TrpI protein with its DNA-binding sites. J. Bacteriol. 173:1590-1597.
6. Crawford, J. A., J. B. Kaper, and V. J. DiRita. 1998. Analysis of ToxR-dependent transcription activation of ompU, the gene encoding a major envelope protein in Vibrio cholerae. Mol. Microbiol. 29:235-246.
7. DiRita, V. J., and J. J. Mekalanos. 1991. Periplasmic interaction between two membrane regulatory proteins. ToxR and ToxS, results in signal transduction and transcriptional activation. Cell 64:29-37.
8. DiRita, V. J., M. Neely, R. K. Taylor, and P. M. Bruss. 1996. Differential expression of the ToxR regulon in classical and El Tor biotypes of Vibrio cholerae is due to biotype-specific control over toxT expression. Proc. Natl. Acad. Sci. USA 93:7991-7995.
9. DiRita, V. J., C. Parsot, G. Jander, and J. J. Mekalanos. 1991. Regulatory cascade controls virulence in Vibrio cholerae. Proc. Natl. Acad. Sci. USA 88:5403-5407.
10. Frohman, M. A., M. K. Dush, and G. R. Martin. 1988. Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer. Proc. Natl. Acad. Sci. USA 85:8998-9002.
11. Gao, J., and G. N. Gussin. 1991. Activation of the trpBA promoter of Pseudomonas aeruginosa by the TrpI protein in vitro. J. Bacteriol. 173:3763-3769.
12. Gardel, C. L., and J. J. Mekalanos. 1994. Regulation of cholera toxin by temperature, pH and osmolarity. Methods Enzymol. 235:517-526.
13. Häse, C. C., and J. J. Mekalanos. 1998. TcpP protein is a positive regulator of virulence gene expression in Vibrio cholerae. Proc. Natl. Acad. Sci. USA 95:730-734.
14. Higgins, D. E., and V. J. DiRita. 1994. Transcriptional control of toxT, a regulatory gene in the ToxR regulon of Vibrio cholerae. Mol. Microbiol. 14:17-29.
15. Higgins, D. E., E. Nazareno, and V. J. DiRita. 1992. The virulence gene activator ToxT from Vibrio cholerae is a member of the AraC family of transcriptional activators. J. Bacteriol. 174:6974-6980.
16. Iwanaga, M., K. Yamamoto, N. Higa, Y. Ichinose, N. Nakasone, and M. Tanabe. 1986. Culture conditions for stimulating cholera toxin production by Vibrio cholerae O1 El Tor. Microbiol. Immunol. 30:1075-1083.
17. Kaper, J. B., J. G. Morris, Jr., and M. M. Levine. 1995. Cholera. Clin. Microbiol. Rev. 8:48-86.
18. Karaolis, D. K. R., J. A. Johnson, C. C. Bailey, E. C. Boedeker, J. B. Kaper, and P. R. Reeves. 1998. A Vibrio cholerae pathogenicity island associated with epidemic and pandemic strains. Proc. Natl. Acad. Sci. USA 95:3134-3139.
19. Karaolis, D. K. R., S. Somara, D. R. Maneval, Jr., J. A. Johnson, and J. B. Kaper. 1999. A bacteriophage encoding a pathogenicity island, a type-IV pilus and a phage receptor in cholera bacteria. Nature 399:375-379.
20. Kovach, M. E., M. D. Shaffer, and K. M. Peterson. 1996. A putative integrase gene defines the distal end of a large cluster of ToxR-regulated colonization genes in Vibrio cholerae. Microbiology 142:2165-2174.
21. Kovacikova, G., and K. Skorupski. 1999. A Vibrio cholerae LysR homolog, AphB, cooperates with AphA at the tcpPH promoter to activate expression of the ToxR virulence cascade. J. Bacteriol. 181:4250-4256.
22. Mares, R., M. L. Urbanowski, and G. V. Stauffer. 1992. Regulation of the Salmonella typhimurium metA gene by the MetR protein and homocysteine. J. Bacteriol. 174:390-397.
23. Miller, J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
24. Miller, V. L., V. J. DiRita, and J. J. Mekalanos. 1989. Identification of toxS, a regulatory gene whose product enhances ToxR-mediated activation of the cholera toxin promoter. J. Bacteriol. 171:1288-1293.
25. Miller, V. L., and J. J. Mekalanos. 1988. A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR. J. Bacteriol. 170:2575-2583.
26. Miller, V. L., R. K. Taylor, and J. J. Mekalanos. 1987. Cholera toxin transcriptional activator ToxR is a transmembrane DNA binding protein. Cell 48:271-279.
27. Murley, Y. M., P. A. Carroll, K. Skorupski, R. K. Taylor, and S. B. Calderwood. 1999. Differential transcription of the tcpPH operon confers biotype-specific control of the Vibrio cholerae ToxR virulence regulon. Infect. Immun. 67:5117-5123.
28. Ogierman, M. A., E. Voss, C. Meaney, R. Faast, S. R. Attridge, and P. A. Manning. 1996. Comparison of the promoter proximal regions of the toxin-co-regulated tcp gene cluster in classical and El Tor strains of Vibrio cholerae O1. Gene 170:9-16.
29. Padgett, K. A., and J. A. Sorge. 1996. Creating seamless junctions independent of restriction sites in PCR cloning. Gene 168:31-35.
30. Schell, M. A. 1993. Molecular biology of the LysR family of transcriptional regulators. Annu. Rev. Microbiol. 47:597-626.
31. Skorupski, K., and R. K. Taylor. 1996. Positive selection vectors far allelic exchange. Gene 169:47-52.
32. Skorupski, K., and R. K. Taylor. 1997. Cyclic AMP and its receptor protein negatively regulate the coordinate expression of cholera toxin and toxin-coregulated pilus in Vibrio cholerae. Proc. Natl. Acad. Sci. USA 94:265-270.
33. Skorupski, K., and R. K. Taylor. 1999. A new level in the Vibrio cholerae ToxR virulence cascade: AphA is required for transcriptional activation of the tcpPH operon. Mol. Microbiol. 31:763-771.
34. Sun, D., J. M. Seyer, I. Kovari, R. A. Sumrada, and R. K. Taylor. 1991. Localization of protective epitopes within the pilin subunit of the Vibrio cholerae toxin-coregulated pilus. Infect. Immun. 59:114-118.
35. Taylor, R. K., V. L. Miller, D. B. Furlong, and J. J. Mekalanos. 1987. Use of phoA gene fusions to identify a pilus colonization factor coordinately regulated with cholera toxin. Proc. Natl. Acad. Sci. USA 84:2833-2837.
36. Urbanowski, M. L., and G. V. Stauffer. 1989. Genetic and biochemical analysis of the MetR activator-binding site in the metE metR control region of Salmonella typhimurium. J. Bacteriol. 171:5620-5629.
37. Waldor, M. K., and J. J. Mekalanos. 1996. Lysogenic conversion by a filamentous phage encoding cholera toxin. Science 272:1910-1914.
38. Yu, R. R., and V. J. DiRita. 1999. Analysis of an autoregulatory loop controlling ToxT, cholera toxin, and toxin-coregulated pilus production in Vibrio cholerae. J. Bacteriol. 181:2584-2592.