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Volumn 272, Issue 5270, 1996, Pages 1910-1913

Lysogenic conversion by a filamentous phage encoding cholera toxin

Author keywords

[No Author keywords available]

Indexed keywords

CHOLERA TOXIN; ENTEROTOXIN; RECEPTOR PROTEIN;

EID: 0030057090     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.272.5270.1910     Document Type: Article
Times cited : (1441)

References (34)
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    • note
    • The primers used to determine the sequence of the (+) DNA strand upstream of cep were 5′-CGGAAAACAGCAAGAAAAC-3′ and 5′-AGAACAGAACGAGAACGG-3′. The primers used to determine the DNA sequence of the (-) DNA strand downstream of ctxB were 5′-AATAATAAAACGCCTCAT-3′ and 5′-CGATGATGAGAAGCAACC-3′. Dye terminator cycle sequencing with a kit using AmpliTaq DNA polymerase FS (Perkin-Elmer) was carried out according to the manufacturer's recommendations. DNA sequences were determined with an ABI 373A DNA sequencer.
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    • note
    • This work was supported by NIH grants Al 18045 to J.J.M. and A101321 to M.K.W. We thank D. Furlong for invaluable help with the electron microscope and D. Steiger for help with the DNA sequencing. We are grateful to Mekalanos laboratory members, especially E. Rubin, J. Tobias, S. Chiang, and G. Pearson, for valuable support, suggestions, and strains. All experiments involving animals were done in accordance with the guidelines for animal experimentation of Harvard Medical School.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.