Examination of Salmonella gene expression in an infected mammalian host using the green fluorescent protein and two-colour flow cytometry

Molecular Microbiology

Volumn 43, Issue 5, 2002, Pages 1269-1283

  Bumann, Dirk ^a  

^a MAX PLANCK INSTITUTE FOR INFECTION BIOLOGY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

AROMATIC CARBOXYLIC ACID; CHORISMATE MUTASE; GREEN FLUORESCENT PROTEIN;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BACTERIAL COLONIZATION; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIAL VIRULENCE; BIOSYNTHESIS; CONTROLLED STUDY; FEMALE; FLOW CYTOMETRY; GENE ACTIVITY; GENE DELETION; GENE EXPRESSION; GENE INDUCTION; GENE LIBRARY; GENE SEQUENCE; GENETIC ANALYSIS; IN VIVO STUDY; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PREDICTION; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; SALMONELLA; SALMONELLOSIS;

ANIMALS; BACTERIAL PROTEINS; CULTURE MEDIA; FEMALE; FLOW CYTOMETRY; GENE EXPRESSION REGULATION, BACTERIAL; GREEN FLUORESCENT PROTEINS; LUMINESCENT PROTEINS; MICE; MICE, INBRED BALB C; PROMOTER REGIONS (GENETICS); RECOMBINATION, GENETIC; SALMONELLA; SALMONELLA INFECTIONS, ANIMAL; VIRULENCE;

ANIMALIA; BACTERIA (MICROORGANISMS); MAMMALIA; NEGIBACTERIA; SALMONELLA;

EID: 0036229497     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.2002.02821.x     Document Type: Article

References (67)

1. Salmonella SirA is a global regulator of genes mediating enteropathogenesis
2. Salmonella typhimurium activates virulence gene transcription within acidified macrophage phagosomes
3. Salmonella maintains the integrity of its intracellular vacuole through the action of SifA
4. In vivo genetic analysis indicates that PhoP-PhoQ and the Salmonella pathogenicity island 2 type III secretion system contribute independently to Salmonella enterica serovar Typhimurium virulence
5. At least four percent of the Salmonella typhimurium genome is required for fatal infection of mice
6. Fluorescence correlation microscopy of cells in the presence of autofluorescence
7. In vivo visualization of bacterial colonization, antigen expression, and specific T-cell induction following oral administration of live recombinant Salmonella enterica serovar Typhimurium
8. Cell-specific proteins synthesized by Salmonella typhimurium
9. The emerging periplasm-localized subclass of AroQ chorismate mutases, exemplified by those from Salmonella typhimurium and Pseudomonas aeruginosa
10. Macrophage-dependent induction of the Salmonella pathogenicity island 2 type III secretion system and its role in intracellular survival
11. Improved detection of bacteria by flow cytometry using a combination of antibody and viability markers
12. FACS-optimized mutants of the green fluorescent protein (GFP)
13. Bacterial virulence gene regulation: An evolutionary perspective
14. InvF is required for expression of genes encoding proteins secreted by the SPI1 type III secretion apparatus in Salmonella typhimurium
15. The putative invasion protein chaperone SicA acts together with InvF to activate the expression of Salmonella typhimurium virulence genes
16. Flow cytometric analysis of gramnegative bacterial cells
17. Cloning and molecular characterization of genes whose products allow Salmonella typhimurium to penetrate tissue culture cells
18. Mg2+ as an extracellular signal: Environmental regulation of Salmonella virulence
19. How Salmonella became a pathogen
20. Characterization of the Salmonella typhimurium pagC/pagD chromosomal region
21. Whole genome scan for habitat-specific genes by signature-tagged mutagenesis
22. MicroReview: Salmonella pathogenicity island 2
23. Genes encoding putative effector proteins of the type III secretion system of Salmonella pathogenicity island 2 are required for bacterial virulence and proliferation in macrophages
24. The Salmonella invasin SipB induces macrophage apoptosis by binding to caspase-1
25. A stable shuttle vector system for efficient genetic complementation of Helicobacter pylori strains by transformation and conjugation
26. Aromatic-dependent Salmonella typhimurium are non-virulent and effective as live vaccines
27. Construction of genetically defined double aro mutants of Salmonella typhi
28. Dendritic cells of the murine Peyer's patches colocalize with Salmonella typhimurium avirulent mutants in the subepithelial dome
29. A recombinant Salmonella typhimurium vaccine strain is taken up and survives within murine Peyer's patch dendritic cells
30. Gene splicing by overlap extension
31. A proteinaceous gene regulatory thermometer in Salmonella
32. In vitro and in vivo expression studies of yopE from Yersinia enterocolitica using the gfp reporter gene
33. Identification and characterization of a Salmonella typhimurium oxygen-regulated gene required for bacterial internalization
34. Salmonellosis: Host immune responses and bacterial virulence determinants
35. Flagellar proteins and type III-exported virulence factors are the predominant proteins secreted into the culture media of Salmonella typhimurium
36. OmpR regulates the two-component system SsrA-SsrB in Salmonella pathogenicity island 2
37. A secreted Salmonella protein induces a proinflammatory response in epithelial cells, which promotes neutrophil migration
38. Novel approaches to monitor bacterial gene expression in infected tissue and host
39. Regulation and temporal expression patterns of Vibrio cholerae virulence genes during infection
40. Unravelling the mysteries of virulence gene regulation in Salmonella typhimurium
41. Complete genome sequence of Salmonella enterica serovar Typhimurium LT2
42. Overproduction of proteins using expression-cassette polymerase chain reaction
43. Selection of bacterial virulence genes that are specifically induced in host tissues
44. Assessment of bacterial pathogenesis by analysis of gene expression in the host
45. Constitutive expression of the phoP regulon attenuates Salmonella virulence and survival within macrophages
46. A two-component regulatory system (phoP/phoQ) controls Salmonella typhimurium virulence
47. An unusual pagC::TnphoA mutation leads to an invasion- and virulence-defective phenotype in Salmonellae
48. Analysis of bacterial function by multi-colour fluorescence flow cytometry and single cell sorting
49. Salmonella typhimurium virulence genes are induced upon bacterial invasion into phagocytic and nonphagocytic cells
50. Cryptococcus neoformans differential gene expression detected in vitro and in vivo with green fluorescent protein
51. Murine salmonellosis studied by confocal microscopy: Salmonella typhimurium resides intracellularly inside macrophages and exerts a cytotoxic effect on phagocytes in vivo
52. Intracellular replication of Salmonella typhimurium strains in specific subsets of splenic macrophages in vivo
53. Characterization of the integration site of Yersinia high-pathogenicity island in Escherichia coli
54. Coupling bacterial activity measurements with cell sorting by flow cytometry
55. The codon adaptation index - A measure of directional synonymous codon usage bias, and its potential applications
56. Flow cytometry of bacteria: Glimpses from the past with a view to the future
57. Avirulence of LT2 strains of Salmonella typhimurium results from a defective rpoS gene
58. Safety of live oral Salmonella typhi vaccine strains with deletions inhtrA and aroC aroD and immune response in humans
59. Fluorescence-based isolation of bacterial genes expressed within host cells
60. Applications of gene fusions to green fluorescent protein and flow cytometry to the study of bacterial gene expression in host cells
61. Salmonella pathogenicity island 2-dependent evasion of the phagocyte NADPH oxidase
62. Rapid detection of Salmonella in chicken washes by immunomagnetic separation and flow cytometry
63. An altered rpoS allele contributes to the avirulence of Salmonella typhimurium LT2
64. Identification of a pathogenicity island required for Salmonella enteropathogenicity
65. Visualizing gene expression by whole-body fluorescence imaging
66. In vivo activation of dendritic cells and T cells during Salmonella enterica serovar Typhimurium infection
67. An invasion-associated Salmonella protein modulates the actin-bundling activity of plastin