Fluorescence-based isolation of bacterial genes expressed within host cells

Science

Volumn 277, Issue 5334, 1997, Pages 2007-2011

  Valdivia, Raphael H ^a   Falkow, Stanley ^a  

^a STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACTERIAL GENE; BACTERIAL GENETICS; BACTERIAL VIRULENCE; FLUORESCENCE; GENE EXPRESSION; GENETIC ANALYSIS; HOST CELL; MACROPHAGE; NONHUMAN; PRIORITY JOURNAL; SALMONELLA TYPHIMURIUM;

ANIMALS; BACTERIAL PROTEINS; CELL LINE; CLONING, MOLECULAR; FEMALE; FLOW CYTOMETRY; FLUORESCENCE; GENE EXPRESSION REGULATION, BACTERIAL; GREEN FLUORESCENT PROTEINS; HELA CELLS; HUMANS; LUMINESCENT PROTEINS; MACROPHAGES; MICE; MICE, INBRED BALB C; MICROSCOPY, FLUORESCENCE; MOLECULAR SEQUENCE DATA; OPEN READING FRAMES; PROMOTER REGIONS (GENETICS); RECOMBINANT FUSION PROTEINS; SALMONELLA INFECTIONS, ANIMAL; SALMONELLA TYPHIMURIUM; SPLEEN; TRANSCRIPTION FACTORS; VIRULENCE;

EID: 0030866940     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.277.5334.2007     Document Type: Article

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41. We thank L. Ramakrishnan, E. Strauss, J. Mecsas, N. Salama, A. Covacci, and D. Monack for critical reading of the manuscnpt; J. Shea and D. Holden for sharing strains and unpublished information; C. Johnson for DNA sequencing support; and L. Roitt at the Digestive Disease Center FACS facility. Supported by U.S. Public Health Service grant AI 26195 and Stanford's Digestive Disease Center grant DK38707.