A stable shuttle vector system for efficient genetic complementation of Helicobacter pylori strains by transformation and conjugation

Molecular and General Genetics

Volumn 257, Issue 5, 1998, Pages 519-528

  Heuermann, D ^a,c   Haas, R ^a,b  

^a MAX PLANCK INST FÜR BIOLOGIE   (Germany)

^b UNIVERSITY OF MUNICH   (Germany)

^c ALTANA PHARMA AG   (Germany)

Author keywords

Bacterial conjugation; Green fluorescent protein; Homologous recombination; Natural transformation competence; Plasmid vector

Indexed keywords

GREEN FLUORESCENT PROTEIN; PLASMID DNA; PLASMID VECTOR;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL VIRULENCE; BACTERIUM CONJUGATION; CONTROLLED STUDY; DNA REPLICATION; DNA REPLICATION ORIGIN; ESCHERICHIA COLI; GENE CASSETTE; GENE EXPRESSION; GENE MAPPING; GENETIC COMPLEMENTATION; HELICOBACTER PYLORI; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; SHUTTLE VECTOR;

HELICOBACTER PYLORI;

EID: 0031960996     PISSN: 00268925     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004380050677     Document Type: Article

References (35)

1. Appelmelk BJ et al. (1996) Potential role of molecular mimicry between Helicobacter pylori lipopolysaccharide and host Lewis blood group antigens in autoimmunity. Infect Immun 64:2031-2040
2. Biswas GD, Burnstein KL, Sparling PF (1986) Linearization of donor DNA during plasmid transformation in Neisseria gonorrhoeas. J Bacteriol 168:756-761
3. Boyer HW, Roulland-Dussoix D (1969) A complementation analysis of the restriction and modification of DNA in Escherichia coli. J Mol Biol 41:459-472
4. Chalfie M, Tu Y, Euskirchen G, Ward WW, Prasher DC (1994) Green fluorescent protein as a marker for gene expression. Science 263:802-805
5. Cormack BP, Valdivia RH, Falkow S (1996) FACS-optimized mutants of the green fluorescent protein (gfp). Gene 173:33-38
6. Cover TL (1996) The vacuolating cytotoxin of Helicobacter priori. Mol Microbiol 20:241-246
7. Delagrave S, Hawtin RE, Silva CM, Yang MM, Youvan DC (1995) Red-shifted excitation mutants of the green fluorescent protein. Bio/Technology 13:151-154
8. Eaton KA, Krakowa S (1994) Effect of gastric pH on urease-dependent colonization of gnotobiotic piglets by Helicobacter pylori. Infect Immun 62:3604-3607
9. Feinberg AP, Vogelstein B (1983) A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Analytical Biochemistry 132:6-13
10. Forman D et al. (1993) An international association between Helicobacter pylori infection and gastric cancer. Lancet 341:1359-1362
11. Haas R, Kahrs AF, Facius D, Allmeier H, Schmitt R, Meyer TF (1993a) TnMax - a versatile mini-transposon for the analysis of cloned genes and shuttle mutagenesis. Gene 130:23-31
12. Haas R, Meyer TF, van Putten JPM (1993b) Aflagellated mutants of Helicobacter priori generated by genetic transformation of naturally competent strains using transposon shuttle mutagenesis. Mol Microbiol 8:753-760
13. Hentschel E, Nemec H, Schutze K, Hirschl A, Dragosics B, Brandstatter G, Taufer M (1991) Duodenal ulcer recurrence and Helicobacter pylori. Lancet 338:569
14. Heuermann D, Haas R (1995) Genetic organization of a small cryptic plasmid of Helicobacter pylori. Gene 165:17-24
15. Kahn M, Kolter R, Thomas C, Figurski D, Meyer R, Remaut E, Helinski DR (1979) Plasmid cloning vehicles derived from plasmids ColE1, F, R6K, and RK2. Methods Enzymol 68: 268-280
16. Kahrs AF, Odenbreit S, Schmitt W, Heuermann D, Meyer TF, Haas R (1995) An improved TnMax mini-transposon system suitable for sequencing, shuttle mutagenesis and gene fusions. Gene 167:53-57
17. Kleanthous H, Clayton CL, Tabaqchali S (1991) Characterization of a plasmid from Helicobacter pylori encoding a replication protein common to plasmids in Gram-positive bacteria. Mol Microbiol 5:2377-2389
18. Labigne A, Cussac V, Courcoux P (1991) Shuttle cloning of genes responsible for urease activity from Helicobacter pylori and nucleotide sequence determination. J Bacteriol 173:1920-1931
19. Labigne-Roussel A, Harel J, Tompkins L (1987) Gene transfer from Escherichia coli to Campylobacter species: development of shuttle vectors for genetic analysis of Campylobacter jejuni. J Bacteriol 169:5320-5323
20. Leying H, Suerbaum S, Geis G, Haas R (1992) Characterisation of flaA, a Helicobacter pylori flagellin gene. Mol Microbiol 6:2863-2874
21. Marsh JL, Erfle M, Wykes EJ (1984) The pIC plasmid and phage vectors with versatile cloning sites for recombinant selection by insertional inactivation. Gene 32:481-485
22. Minnis JA, Taylor TE, Knesek JE, Peterson WL, Mcintire SA (1995) Characterization of a 3.5-kbp plasmid from Helicobacter pylori. Plasmid 34:22-36
23. Nordström K (1990) Control of plasmid replication how do DNA iterons set the replication frequency. Cell 63:1121-1124
24. Odenbreit S, Till M, Haas R (1996) Optimized BlaM-transposon shuttle mutagenesis of Helicobacter pylori allows the identification of novel genetic loci involved in bacterial virulence. Mol Microbiol 20:361-373
25. Phadnis SH, Westblom TU, Normark S (1993) Molecular cloning of Helicobacter pylori DNA: important differences between mcrC deletion host strains. Mol Microbiol 10:1151
26. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
27. Schmitt W, Odenbreit S, Heuermann D, Haas R (1995) Cloning of the Helicobacter pylori recA gene and functional characterization of its product. Mol Gen Genet 248:563-572
28. Southern EM (1975) Detection of specific sequences among DNA fragments separated by agarose gel electrophoresis. J Mol Biol 98:503-517
29. Suerbaum S (1995) The complex flagella of gastric Helicobacter species. Trends Microbiol 3:168-170
30. Taha MK, So M, Seifert HS, Billyard E, Marchai C (1988) Pilin expression in Neisseria gonorrhoeae is under both positive and negative transcriptional control. EMBO J 7:4367-4378
31. Thomas CM (1981) Molecular genetics of broad host range plasmid RK2. Plasmid 5:10-19
32. Trieu-Cuot P, Gerbaud G, Lambert T, Courvalin P (1985) In vivo transfer of genetic information between Gram-positive and Gram-negative bacteria. EMBO J 4:3583-3587
33. Tsuda M, Karita M, Morshed MG, Okita K, Nakazawa T (1994) A urease-negative mutant of Helicobacter pylori constructed by allelic exchange mutagenesis lacks the ability to colonize the nude mouse stomach. Infect Immun 62:3586-3589
34. Wadström T, Ascencio F, Ljungh A, Lelwala-Guruge J, Ringnér M, Utt M, Valkonen K (1993) Helicobacter pylori adhesins. Eur J Gastroenterol Hepatol 5 (Suppl 2):S12-S15
35. Wang Y, Roos KP, Taylor DE (1993) Transformation of Helicobacter pylori by chromosomal metronidazole resistance and by a plasmid with a selectable chloramphenicol resistance marker. J Gen Microbiol 13:2485-2493