Effects of mutations in the adenoviral E1B 55-kilodalton protein coding sequence on viral late mRNA metabolism

Journal of Virology

Volumn 76, Issue 9, 2002, Pages 4507-4519

  Gonzalez, Ramon A ^a   Flint, S J ^a  

^a PRINCETON UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; MESSENGER RNA; VIRUS PROTEIN;

ADENOVIRUS; AMINO ACID SEQUENCE; ARTICLE; CELL NUCLEUS; CONTROLLED STUDY; CYTOPLASM; DNA REPLICATION; DNA TRANSCRIPTION; EMBRYO; GENE INSERTION; GENETIC CODE; HUMAN; HUMAN CELL; NONHUMAN; PLEIOTROPY; PRIORITY JOURNAL; PROTEIN FOLDING; PROTEIN PROTEIN INTERACTION; RNA METABOLISM; RNA TRANSPORT; VIRUS MUTATION;

ADENOVIRUS E1B PROTEINS; ADENOVIRUS E4 PROTEINS; ADENOVIRUSES, HUMAN; CELL LINE; GENE EXPRESSION REGULATION, VIRAL; HELA CELLS; HUMANS; MUTATION; RNA, MESSENGER; RNA, VIRAL; TRANSCRIPTION, GENETIC; VIRAL PROTEINS;

EID: 0036226061     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/JVI.76.9.4507-4519.2002     Document Type: Article

References (82)

1. Organization of splicing factors in adenovirus-infected cells reflects changes in gene expression during the early to late phase transition
2. Adenovirus E1B proteins are required for accumulation of late viral mRNA and for effects on cellular mRNA translation and transport
3. The C-terminal domain of TAP interacts with the nuclear pore complex and promotes export of specific CTE-bearing RNA substrates
4. NF-κB: Ten years after
5. Adenovirus proteins from both E1B reading frames are required for transformation of rodent cells by viral infection and DNA transfection
6. An adenovirus mutant that replicates selectively in p53-deficient human tumor cells
7. The intracellular distribution of the transformation-associated protein p53 in adenovirus-transformed rodent cells
8. How proteins move across the endoplasmic reticulum membrane
9. Analysis of synthesis, stability, phosphorylation, and interacting polypeptides of the 34-kilodalton product of open reading frame 6 of the early region 4 protein of human adenovirus type 5
10. Interaction of adenoviral E4 and E1b products in late gene expression
11. Nuclear organization of adenovirus RNA biogenesis
12. Spliced exons of adenovirus late RNAs colocalize with snRNP in a specific nuclear domain
13. Effects of adenovirus infection on rRNA synthesis and maturation in HeLa cells
14. 2 initiator
15. Analysis of adenovirus early region 4-encoded polypeptides synthesized in productively infected cells
16. Jak-STAT pathways and transcriptional activation in response to IFNs and other extracellular signaling proteins
17. Nuclear targeting sequences - A consensus?
18. Nuclear export of the E1B 55-kDa and E4 34-kDa adenoviral oncoproteins mediated by a rev-like signal sequence
19. Blockage by adenovirus E4orf6 of transcriptional activation by the p53 tumor suppressor
20. Nuclear export of adenovirus RNA
21. The adenovirus type 5 E1B-55k oncoprotein actively shuttles in virus-infected cells, whereas transport of E4orf6 is mediated by a CRM1-independent mechanism
22. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
23. Regulation of adenovirus mRNA formation
24. E1B 55-kilodalton-associated protein: A cellular protein with RNA-binding activity implicated in nucleocytoplasmic transport of adenovirus and cellular mRNAs
25. The early region 1B 55-kilodalton oncoprotein of adenovirus relieves growth restrictions imposed on viral replication by the cell cycle
26. p53 status does not determine outcome of E1B 55-kilodalton mutant adenovirus lytic infection
27. Roles for the E4 orf6, orf3, and E1B 55-kilodalton proteins in cell cycle-independent adenovirus replication
28. p53-independent and -dependent requirements for E1B-55K in adenovirus type 5 replication
29. Host range mutants of adenovirus type 5 defective for growth on HeLa cells
30. The adenovirus L4 100-kilodalton protein is necessary for efficient translation of viral late mRNA species
31. Isolation of type 5 adenovirus mutants with a cold-sensitive phenotype: Genetic evidence of an adenovirus transformation maintenance function
32. RNA-binding activity of the E1B 55-kilodalton protein from human adenovirus type 5
33. Transformation by the human adenoviruses
34. Domains required for in vitro association between the cellular p53 and the adenovirus 2 E1B 55K proteins
35. Anatomy of an unusual RNA polymerase II promoter containing a downstream TATA element
36. p53: Puzzle and paradigm
37. The adenovirus type 5 E1B-55K oncoprotein is a highly active shuttle protein and shuttling is independent of E4orf6, p53 and Mdm2
38. Regulated RNA processing and RNA transport during adenovirus infection
39. Selective effects on adenovirus late gene expression of deleting the E1B 55K protein
40. The adenovirus type 5 E1b 55K and E4 Orf3 proteins associate in infected cells and affect ND10 components
41. The adenovirus E1B 55 kd protein influences mRNA transport via an intranuclear effect on RNA metabolism
42. p53, the cellular gatekeeper for growth and division
43. Studies on the phosphorylation of the 58000 dalton early region 1B protein of human adenovirus type 5
44. Adenovirus E1B 55K represses p53 activation in vitro
45. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange
46. Oncogenic potential of the adenovirus E4orf6 protein
47. Complete nucleotide sequence of a milk-transmitted mouse mammary tumor virus: Two frameshift suppression events are required for translation of gag and pol
48. The adenovirus E4-6/7 protein transactivates the E2 promoter by inducing dimerization of a heteromeric E2F complex
49. Location of the adenovirus early region 1B 55-kilodalton protein during lytic infection: Association with nuclear viral inclusions requires the early region 4 34-kilodalton protein
50. The adenovirus E1B-55K transforming polypeptide modulates transport or cytoplasmic stablization of viral and host cell mRNAs
51. Adenovirus replication and transcription sites are spatially separated in the nucleus of infected cells
52. Chromatin structure, DNA synthesis and transcription through the lifespan of human embryonic lung fibroblasts
53. Regulation of p53 levels by the E1B 55-kilodalton protein and E4orf6 in adenovirus-infected cells
54. Adenovirus late gene expression does not require a Rev-like nuclear RNA export pathway
55. Monoclonal antibodies which recognise native and denatured forms of the adenovirus DNA-binding protein
56. Inactivation of p53 but not p73 by adenovirus type 5 E1B 55-kilodalton and E4 34-kilodalton oncoproteins
57. Replication of ONYX-015, a potential anticancer adenovirus, is independent of p53 status in tumor cells
58. The kinetics of synthesis of early viral proteins in KB cells infected with wild-type and transformationdefective host-range mutants of human adenovirus type 5
59. Structural analysis of the adenovirus type 5 E1B 55-kilodalton-E4orf6 protein complex
60. Adenovirus early region 1B 58,000-dalton tumor antigen is physically associated with an early region 4 25,000-dalton protein in productively infected cells
61. A monoclonal antibody detecting the Ad5 E1B-58K tumor antigen in adenovirus-infected and transformed cells
62. Analyses of single-amino-acid substitution mutants of adenovirus type 5 E1B-55K protein
63. Adenoviridae and their replication
64. The metabolism of small cellular RNA species during productive subgroup C adenovirus infection
65. Intranuclear location of the adenovirus type 5 E1B 55-kilodalton protein
66. The large E1B protein together with the E4orf6 protein target p53 for active degradation in adenovirus infected cells
67. Characterization of the 55K adenovirus type 5 E1B product and related proteins
68. Regulation of p53-dependent apoptosis, transcriptional repression, and cell transformation by phosphorylation of the 55-kilodalton E1B protein of human adenovirus type 5
69. Phosphorylation at the carboxy terminus of the 55-kilodalton adenovirus type 5 E1B protein regulates transforming activity
70. The product of the adenovirus intermediate gene IVa2 is a transcription activator of the major late promoter
71. The replicative capacities of large E1B-null group A and group C adenoviruses are independent of host cell p53 status
72. Association between the cellular p53 and the adenovirus 5 E1B-55kd proteins reduces the oncogenicity of Ad-transformed cells
73. Identification of two nuclear subclasses of the adenovirus type 5-encoded DNA-binding protein
74. Importins and exportins: How to get in and out of the nucleus
75. E1B 55-kilodalton oncoproteins of adenovirus types 5 and 12 inactivate and relocalize p53, but not p51 or p73, and cooperate with E4orf6 proteins to destabilize p53
76. The adenovirus E1B 495R protein plays a role in regulating the transport and stability of the viral late messages
77. Oncogenic transformation of hamster embryo cells in vitro by adenovirus type 5
78. mRNA export correlates with activation of transcription in human subgroup C adenovirus-infected cells
79. Identification of human adenovirus early region 1 products by using antisera against synthetic peptides corresponding to the predicted carboxy termini
80. Inhibition of p53 transactivation required for transformation by adenovirus early 1B protein
81. Dissection of functional domains in the adenovirus 2 early 1B 55K polypeptide by suppressor-linker-insertional mutagenesis
82. Localization of the E1B proteins of adenovirus 5 in transformed cells, as revealed by interaction with monoclonal antibodies