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Human MCF7 cells were either mock-infected or infected with adenovirus at a multiplicity of 50 (wt300) or 250 plaque-forming units per cell (d/338 and d/355). Extracts were prepared 36 hours later and subjected to immunoprecipitation with the use of the p53-specific antibody DO-1. Immunoprecipitated proteins were analyzed by protein immunoblot assay with RSA3 antibody.
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II31 fusion protein were analyzed by protein immunoblot assay with a mixture of antibodies to p53 (mAb 421) and E4orf6 (RSA3).
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We thank N. Kley, H. Lu, J. Lin, and A. Levine for plasmids, A. Levine for critical reading of the manuscript, J. Gong for able technical assistance, and T.D. thanks H. Wolf for support in Regensberg. This work was supported by grants from NIH (T.S.) and the Infektionsforschung AIDS-Stipendienprogramm DKFZ (T.D.). S.R. received scholarship support from the University of Regensberg, and T.S. is an American Cancer Society Professor and an Investigator of the Howard Hughes Medical Institute.
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