Level of the RNA polymerase II in the fission yeast stays constant but phosphorylation of its carboxyl terminal domain varies depending on the phase and rate of cell growth

Genes to Cells

Volumn 7, Issue 3, 2002, Pages 273-284

  Sakurai, Hitomi ^a,b   Ishihama, Akira ^a  

^a NATIONAL INSTITUTE OF GENETICS   (Japan)

^b Japan Science and Techlogy Corporation   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CORE PROTEIN; DNA DIRECTED RNA POLYMERASE; DNA DIRECTED RNA POLYMERASE III; HEPTAPEPTIDE; PROTEIN SUBUNIT; RNA POLYMERASE II; SERINE; THREONINE; TYROSINE;

ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL CULTURE; CELL GROWTH; CELLULAR DISTRIBUTION; CONTROLLED STUDY; ENZYME ACTIVITY; GENETIC TRANSCRIPTION; GROWTH CURVE; GROWTH RATE; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN CONFORMATION; PROTEIN DOMAIN; PROTEIN PHOSPHORYLATION; PROTEIN SYNTHESIS; SCHIZOSACCHAROMYCES POMBE; YEAST CELL;

PROKARYOTA; SCHIZOSACCHAROMYCES; SCHIZOSACCHAROMYCES POMBE;

EID: 0036201722     PISSN: 13569597     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2443.2002.00522.x     Document Type: Article

References (47)

1. A hyperphosphorylated form of RNA polymerase II is the major interphase antigen of the phosphoprotein antibody MPM-2 and interacts with the peptidyl-prolyl isomerase Pin1
2. Cloning and sequence determination of the Schizosaccharomyces pombe rpb1 gene encoding the largest subunit of RNA polymerase II
3. KIN28 encodes a C-terminal domain kinase that controls mRNA transcription in Saccharomyces cerevisiae but lacks cyclin-dependent kinase-activating kinase (CAK) activity
4. Architecture of RNA polymerase II and implications for the transcription mechanism
5. Structural basis of transcription: RNA polymerase II at 2.8Å resolution
6. Phosphorylation of the C-terminal domain of RNA polymerase II
7. Reversible phosphorylation of the C-terminal domain of RNA polymerase II
8. Phosphorylation of the RNA polymerase II largest subunit during heat shock and inhibition of transcription in HeLa cells
9. Heat shock of HeLa cells inactivates a nuclear protein phosphatase specific for dephosphorylation of the C-terminal domain of RNA polymerase II
10. Heat-shock inactivation of the TFIIH-associated kinase and change in the phosphorylation sites on the C-terminal domain of RNA polymerase II
11. Relationship of CDK-activating kinase and RNA polymerase CTD kinase TFIIH
12. 2β subassembly
13. Mutations in a peptidylpropyl-cis/trans-isomerase gene lead to a defect in 3′-end formation of a pre-mRNA in Saccharomyces cerevisiae
14. RNA polymerase II and the integration of nuclear events
15. Subunit assembly of Escherichia coli RNA polymerase
16. Functional modulation of Escherichia coli RNA polymerase
17. Subunits of yeast RNA polymerases: Structure and function
18. RNA polymerase II subunits 2, 3 and 11 form a core subassembly with DNA binding activity
19. Involvement of multiple subunit-subunit contacts in the assembly of RNA polymerase II
20. Intracellular contents and assembly states of all 12 subunits of the RNA polymerase II in the fission yeast Schizosaccharomyces pombe
21. Formation of CTD-phosphatase (Fcp1)/TFIIF/RNA polymerase II (Pol II) complex in Schizosaccharomyces pombe involves direct interaction between Fcp1 and Rpb4 subunit of Pol II
22. Quantitation of RNA polymerase II and its transcription factors in an HeLa cell: Little soluble holoenzyme but significant amounts of polymerases attached to nuclear substructure
23. Different phosphorylated forms of RNA polymerase II and associated mRNA processing factors during transcription
24. Heat shock-induced alterations in phosphorylation of the largest subunit of RNA polymerase II as revealed by monoclonal antibodies CC-3 and MPM-2
25. Phosphorylation of RNA polymerase IIA occurs subsequent to interaction with the promoter and before initiation of transcription
26. CTD kinase large subunit is encoded by CTK1, a gene required for normal growth of Saccharomyces cerevisiae
27. A kinase-cyclin pair in the RNA polymerase II holoenzyme
28. The nonphosphorylated form of RNA polymerase II preferentially associates with the preinitiation complex
29. Bacterial RNA polymerase subunit ω and eukaryotic RNA polymerase subunit RPB6 are sequence, structural, and functional homologs and promote RNA polymerase assembly
30. Phosphocarboxyl-terminal domain binding and the role of a prolyl isomerase in pre-mRNA-3′-end formation
31. Studies on the omega subunit of Escheirchia coli RNA polymerase: Its role I the recovery of denatured enzyme activity
32. Phosphorylation of the RNA polymerase carboxy-terminal domain by the Bur1 cyclin-dependent kinase
33. Phosphorylation of RNA polymer II CTD fragments results in right binding to the WW domain from the yeast prolyl isomerase Ess1
34. The glucocorticoid receptor inhibits NFκB by interfering with serine-2 phosphorylation of the RNA polymerase II carboxy-terminal domain
35. Growth-related changes in phosphorylation of yeast RNA polymrase II
36. The length, phosphorylation state, and primary structure of the RNA polymerase II CTD dictate interactions with mRNA capping enzymes
37. Three RNA polymerase II carboxyl-terminal domain kinases display distinct substrate preferences
38. Transcription organization and mRNA levels of the genes for all 12 subunits of the fission yeast RNA polymerase II
39. The Rpb4 subunit of fission yeast Schizosaccharomyces pombe RNA polymerase II is essential for cell viability and similar in structure to the corresponding subunits of higher eukaryotes
40. Dynamic association of capping enzymes with transcribing RNA polymerase II
41. The yeast carboxyl-terminal repeat domain kinase CTDK-I is a divergent cyclin-cyclin-dependent kinase complex
42. Inhibition of in vivo and in vitro transcription by monoclonal antibodies prepared against wheat germ RNA polymerase II that react with the heptapeptide repeat of eukaryotic RNA polymerase II
43. Yeast nuclear RNA polymerases
44. Exploring RNA polymerase II structure and function
45. The Ess1 prolyl isomerase is linked to chromatin remodeling complexes and general transcription machinery
46. Sequence-specific and phosphorylation-dependent proline isomerization: A potential mitotic regulatory mechanism
47. Studies of nematode TFIIE function reveal a link between Ser-5 phosphorylation of RNA polymerase II and the transition from transcription initiation to elongation